Strain Fitness in Escherichia coli BW25113 around b1691

Experiment: Trimethoprim 0.0025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydiM and ydiN are separated by 226 nucleotidesydiN and ydiB are separated by 11 nucleotidesydiB and aroD are separated by 30 nucleotides b1690: ydiM - predicted transporter (NCBI), at 1,769,095 to 1,770,309 ydiM b1691: ydiN - predicted transporter (RefSeq), at 1,770,536 to 1,771,801 ydiN b1692: ydiB - quinate/shikimate 5-dehydrogenase, NAD(P)-binding (NCBI), at 1,771,813 to 1,772,679 ydiB b1693: aroD - 3-dehydroquinate dehydratase (NCBI), at 1,772,710 to 1,773,468 aroD Position (kb) 1770 1771 1772Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1769.548 kb on - strand, within ydiMat 1769.556 kb on - strand, within ydiMat 1769.561 kb on - strand, within ydiMat 1769.566 kb on - strand, within ydiMat 1769.641 kb on - strand, within ydiMat 1769.646 kb on + strand, within ydiMat 1769.653 kb on - strand, within ydiMat 1769.751 kb on - strand, within ydiMat 1769.770 kb on - strand, within ydiMat 1769.791 kb on - strand, within ydiMat 1769.817 kb on + strand, within ydiMat 1769.817 kb on + strand, within ydiMat 1769.817 kb on - strand, within ydiMat 1769.847 kb on - strand, within ydiMat 1769.847 kb on - strand, within ydiMat 1769.942 kb on - strand, within ydiMat 1769.942 kb on - strand, within ydiMat 1769.951 kb on + strand, within ydiMat 1770.096 kb on + strand, within ydiMat 1770.106 kb on + strand, within ydiMat 1770.858 kb on - strand, within ydiNat 1770.860 kb on - strand, within ydiNat 1770.860 kb on - strand, within ydiNat 1770.870 kb on - strand, within ydiNat 1770.870 kb on - strand, within ydiNat 1770.870 kb on - strand, within ydiNat 1770.882 kb on - strand, within ydiNat 1770.906 kb on - strand, within ydiNat 1771.124 kb on + strand, within ydiNat 1771.160 kb on + strand, within ydiNat 1771.195 kb on + strand, within ydiNat 1771.269 kb on + strand, within ydiNat 1771.273 kb on + strand, within ydiNat 1771.273 kb on + strand, within ydiNat 1771.273 kb on + strand, within ydiNat 1771.311 kb on + strand, within ydiNat 1771.560 kb on + strand, within ydiNat 1771.564 kb on + strand, within ydiNat 1771.571 kb on + strand, within ydiNat 1771.571 kb on + strand, within ydiNat 1771.859 kb on - strandat 1771.864 kb on - strandat 1771.927 kb on - strand, within ydiBat 1771.950 kb on - strand, within ydiBat 1771.957 kb on - strand, within ydiBat 1771.957 kb on - strand, within ydiBat 1771.964 kb on - strand, within ydiBat 1772.008 kb on - strand, within ydiBat 1772.113 kb on + strand, within ydiBat 1772.113 kb on + strand, within ydiBat 1772.121 kb on + strand, within ydiBat 1772.148 kb on - strand, within ydiBat 1772.163 kb on - strand, within ydiBat 1772.207 kb on - strand, within ydiBat 1772.207 kb on - strand, within ydiBat 1772.610 kb on - strandat 1772.623 kb on - strandat 1772.623 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM
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1,769,548 - ydiM b1690 0.37 +2.2
1,769,556 - ydiM b1690 0.38 +2.2
1,769,561 - ydiM b1690 0.38 +1.5
1,769,566 - ydiM b1690 0.39 +2.2
1,769,641 - ydiM b1690 0.45 +1.2
1,769,646 + ydiM b1690 0.45 +2.2
1,769,653 - ydiM b1690 0.46 +1.2
1,769,751 - ydiM b1690 0.54 +2.2
1,769,770 - ydiM b1690 0.56 +2.2
1,769,791 - ydiM b1690 0.57 +2.2
1,769,817 + ydiM b1690 0.59 +2.2
1,769,817 + ydiM b1690 0.59 +3.2
1,769,817 - ydiM b1690 0.59 +3.2
1,769,847 - ydiM b1690 0.62 +2.2
1,769,847 - ydiM b1690 0.62 +2.2
1,769,942 - ydiM b1690 0.70 +1.2
1,769,942 - ydiM b1690 0.70 +2.2
1,769,951 + ydiM b1690 0.70 +1.2
1,770,096 + ydiM b1690 0.82 +0.6
1,770,106 + ydiM b1690 0.83 +2.2
1,770,858 - ydiN b1691 0.25 +2.2
1,770,860 - ydiN b1691 0.26 +1.2
1,770,860 - ydiN b1691 0.26 +2.2
1,770,870 - ydiN b1691 0.26 +1.2
1,770,870 - ydiN b1691 0.26 +3.1
1,770,870 - ydiN b1691 0.26 +1.0
1,770,882 - ydiN b1691 0.27 +2.2
1,770,906 - ydiN b1691 0.29 +2.2
1,771,124 + ydiN b1691 0.46 +2.2
1,771,160 + ydiN b1691 0.49 +2.2
1,771,195 + ydiN b1691 0.52 -0.4
1,771,269 + ydiN b1691 0.58 +0.6
1,771,273 + ydiN b1691 0.58 +1.2
1,771,273 + ydiN b1691 0.58 -1.8
1,771,273 + ydiN b1691 0.58 +0.2
1,771,311 + ydiN b1691 0.61 +2.2
1,771,560 + ydiN b1691 0.81 +2.2
1,771,564 + ydiN b1691 0.81 -0.1
1,771,571 + ydiN b1691 0.82 +0.6
1,771,571 + ydiN b1691 0.82 -0.4
1,771,859 - -0.1
1,771,864 - +2.2
1,771,927 - ydiB b1692 0.13 -0.4
1,771,950 - ydiB b1692 0.16 +2.2
1,771,957 - ydiB b1692 0.17 -0.4
1,771,957 - ydiB b1692 0.17 +2.2
1,771,964 - ydiB b1692 0.17 +2.2
1,772,008 - ydiB b1692 0.22 +1.2
1,772,113 + ydiB b1692 0.35 -0.6
1,772,113 + ydiB b1692 0.35 -1.1
1,772,121 + ydiB b1692 0.36 +0.8
1,772,148 - ydiB b1692 0.39 -0.4
1,772,163 - ydiB b1692 0.40 +0.5
1,772,207 - ydiB b1692 0.45 +2.2
1,772,207 - ydiB b1692 0.45 +0.5
1,772,610 - +0.2
1,772,623 - +0.9
1,772,623 - +2.2

Or see this region's nucleotide sequence