Strain Fitness in Escherichia coli BW25113 around b1584

Experiment: Trimethoprim 0.0025 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Trimethoprim 0.0025 mM
1,653,461	+	ynfA	b1582	0.28	+0.8
1,653,557	-	ynfA	b1582	0.57	-1.3
1,653,632	+	ynfA	b1582	0.80	-0.0
1,653,757	+				-0.5
1,653,792	+				+0.5
1,653,800	-				-0.7
1,653,869	+	ynfB	b1583	0.11	+0.7
1,653,869	+				+2.3
1,653,881	+	ynfB	b1583	0.14	+0.3
1,654,127	-	ynfB	b1583	0.86	+0.5
1,654,136	+	ynfB	b1583	0.89	-0.2
1,654,144	-				+1.3
1,654,144	-				+1.3
1,654,204	+				-2.2
1,654,204	+				-0.5
1,654,205	-				-0.0
1,654,224	+				-2.4
1,654,263	+				-1.4
1,654,299	-	speG	b1584	0.16	+0.8
1,654,339	+	speG	b1584	0.23	-0.6
1,654,362	+	speG	b1584	0.27	+1.6
1,654,391	-	speG	b1584	0.33	+2.3
1,654,394	+	speG	b1584	0.33	-0.5
1,654,394	+	speG	b1584	0.33	+1.0
1,654,420	+	speG	b1584	0.38	+0.6
1,654,486	+	speG	b1584	0.50	-0.6
1,654,486	+	speG	b1584	0.50	-1.5
1,654,499	+	speG	b1584	0.52	-1.9
1,654,532	+	speG	b1584	0.58	-0.5
1,654,532	+	speG	b1584	0.58	-2.2
1,654,534	-	speG	b1584	0.58	+2.3
1,654,534	-	speG	b1584	0.58	-0.0
1,654,537	+	speG	b1584	0.59	+0.6
1,654,537	+	speG	b1584	0.59	-0.0
1,654,539	+	speG	b1584	0.59	-1.6
1,654,539	+	speG	b1584	0.59	-0.6
1,654,563	+	speG	b1584	0.63	-1.3
1,654,563	+	speG	b1584	0.63	+0.0
1,654,564	+	speG	b1584	0.63	-0.2
1,654,578	+	speG	b1584	0.66	+1.0
1,654,690	+	speG	b1584	0.86	-0.7
1,654,690	+	speG	b1584	0.86	+1.0
1,654,711	+	speG	b1584	0.90	-0.5
1,654,711	+	speG	b1584	0.90	-0.1
1,654,719	-				+0.3
1,654,723	+				-1.8
1,654,723	+				-1.0
1,654,731	-				-0.7
1,654,745	+				-0.3
1,654,745	+				-0.1
1,654,753	-				+0.3
1,654,799	+				-0.2
1,654,807	-				-0.3
1,654,807	-				-1.7
1,654,812	-				+1.3
1,654,812	-				+0.4
1,655,022	-	ynfC	b1585	0.35	-0.4
1,655,058	+	ynfC	b1585	0.40	+2.3
1,655,077	-	ynfC	b1585	0.43	+0.9
1,655,077	-	ynfC	b1585	0.43	+0.7
1,655,102	+	ynfC	b1585	0.47	+0.7
1,655,114	+	ynfC	b1585	0.48	+1.0
1,655,123	-	ynfC	b1585	0.50	-0.1
1,655,123	-	ynfC	b1585	0.50	+1.4
1,655,147	-	ynfC	b1585	0.53	+1.3
1,655,175	-	ynfC	b1585	0.57	-0.5
1,655,199	-	ynfC	b1585	0.60	+1.3
1,655,208	+	ynfC	b1585	0.61	+2.3
1,655,216	-	ynfC	b1585	0.63	-1.3
1,655,394	+	ynfC	b1585	0.88	-0.6
1,655,402	-				+0.3
1,655,402	-	ynfC	b1585	0.89	-0.1
1,655,413	-				+1.3
1,655,428	+				-0.5
1,655,500	-				-2.4
1,655,500	-				-0.6
1,655,626	+	b1586	b1586	0.12	-0.5
1,655,634	-	b1586	b1586	0.15	-1.0
1,655,643	+	b1586	b1586	0.18	-0.2
1,655,645	+	b1586	b1586	0.18	+0.3
1,655,655	+	b1586	b1586	0.22	+0.6
1,655,655	+	b1586	b1586	0.22	+2.7