Strain Fitness in Escherichia coli BW25113 around b1551

Experiment: Trimethoprim 0.0025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntynfO and ydfO are separated by 57 nucleotidesydfO and b1550 are separated by 151 nucleotidesb1550 and b1551 are separated by 171 nucleotidesb1551 and cspI are separated by 345 nucleotidescspI and ydfP are separated by 362 nucleotides b4533: ynfO - Qin prophage; predicted protein (NCBI), at 1,634,780 to 1,635,013 ynfO b1549: ydfO - orf, hypothetical protein (VIMSS), at 1,635,071 to 1,635,481 ydfO b1550: b1550 - orf, hypothetical protein (VIMSS), at 1,635,633 to 1,635,806 b1550 b1551: b1551 - orf, hypothetical protein (VIMSS), at 1,635,978 to 1,636,133 b1551 b1552: cspI - Qin prophage; cold shock protein (NCBI), at 1,636,479 to 1,636,691 cspI b1553: ydfP - Qin prophage; conserved protein (NCBI), at 1,637,054 to 1,637,551 ydfP b4689: rzoQ - hypothetical protein (RefSeq), at 1,637,104 to 1,637,358 rzoQ Position (kb) 1635 1636 1637Strain fitness (log2 ratio) -1 0 1 2at 1635.711 kb on - strand, within b1550at 1635.711 kb on - strand, within b1550at 1635.823 kb on - strandat 1635.823 kb on - strandat 1635.847 kb on - strandat 1635.847 kb on - strandat 1636.078 kb on - strand, within b1551at 1636.097 kb on - strand, within b1551at 1636.212 kb on + strandat 1636.353 kb on - strandat 1636.354 kb on + strandat 1636.421 kb on - strandat 1636.426 kb on - strandat 1636.428 kb on - strandat 1636.434 kb on - strandat 1636.434 kb on - strandat 1636.499 kb on - strandat 1636.500 kb on - strandat 1637.122 kb on - strandat 1637.122 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM
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1,635,711 - b1550 b1550 0.45 +1.1
1,635,711 - b1550 b1550 0.45 +0.3
1,635,823 - +0.3
1,635,823 - +0.6
1,635,847 - +0.7
1,635,847 - +0.4
1,636,078 - b1551 b1551 0.64 +2.3
1,636,097 - b1551 b1551 0.76 +2.3
1,636,212 + +2.3
1,636,353 - -1.0
1,636,354 + +2.3
1,636,421 - +2.3
1,636,426 - +0.2
1,636,428 - +0.7
1,636,434 - +2.3
1,636,434 - +2.3
1,636,499 - +1.3
1,636,500 - +2.3
1,637,122 - +1.3
1,637,122 - +2.3

Or see this region's nucleotide sequence