Experiment: Trimethoprim 0.0025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydcT and ydcU are separated by 0 nucleotides ydcU and ydcV overlap by 11 nucleotides ydcV and ydcW are separated by 21 nucleotides
b1441: ydcT - predicted spermidine/putrescine transporter subunit (NCBI), at 1,510,841 to 1,511,854
ydcT
b1442: ydcU - predicted spermidine/putrescine transporter subunit (NCBI), at 1,511,855 to 1,512,796
ydcU
b1443: ydcV - predicted spermidine/putrescine transporter subunit (NCBI), at 1,512,786 to 1,513,580
ydcV
b1444: ydcW - medium chain aldehyde dehydrogenase (NCBI), at 1,513,602 to 1,515,026
ydcW
Position (kb)
1511
1512
1513 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1510.962 kb on - strand, within ydcT at 1511.021 kb on + strand, within ydcT at 1511.073 kb on + strand, within ydcT at 1511.302 kb on + strand, within ydcT at 1511.365 kb on - strand, within ydcT at 1511.428 kb on - strand, within ydcT at 1511.757 kb on - strand at 1511.782 kb on + strand at 1511.886 kb on + strand at 1511.886 kb on + strand at 1512.028 kb on - strand, within ydcU at 1512.111 kb on - strand, within ydcU at 1512.111 kb on - strand, within ydcU at 1512.133 kb on + strand, within ydcU at 1512.192 kb on - strand, within ydcU at 1512.192 kb on - strand, within ydcU at 1512.194 kb on + strand, within ydcU at 1512.269 kb on - strand, within ydcU at 1512.470 kb on - strand, within ydcU at 1512.754 kb on - strand at 1512.754 kb on - strand at 1512.889 kb on - strand, within ydcV at 1512.964 kb on - strand, within ydcV at 1512.964 kb on - strand, within ydcV at 1513.150 kb on - strand, within ydcV at 1513.161 kb on - strand, within ydcV at 1513.161 kb on - strand, within ydcV at 1513.229 kb on + strand, within ydcV at 1513.229 kb on + strand, within ydcV
Per-strain Table
Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM remove 1,510,962 - ydcT b1441 0.12 +3.0 1,511,021 + ydcT b1441 0.18 -1.7 1,511,073 + ydcT b1441 0.23 -0.3 1,511,302 + ydcT b1441 0.45 -0.7 1,511,365 - ydcT b1441 0.52 -0.5 1,511,428 - ydcT b1441 0.58 +0.8 1,511,757 - -1.0 1,511,782 + -0.0 1,511,886 + -0.2 1,511,886 + -0.1 1,512,028 - ydcU b1442 0.18 -0.5 1,512,111 - ydcU b1442 0.27 -0.1 1,512,111 - ydcU b1442 0.27 -0.7 1,512,133 + ydcU b1442 0.30 -0.9 1,512,192 - ydcU b1442 0.36 +0.7 1,512,192 - ydcU b1442 0.36 -0.9 1,512,194 + ydcU b1442 0.36 -0.2 1,512,269 - ydcU b1442 0.44 -0.4 1,512,470 - ydcU b1442 0.65 -0.7 1,512,754 - +1.3 1,512,754 - +0.3 1,512,889 - ydcV b1443 0.13 +0.3 1,512,964 - ydcV b1443 0.22 +0.0 1,512,964 - ydcV b1443 0.22 -1.4 1,513,150 - ydcV b1443 0.46 -0.7 1,513,161 - ydcV b1443 0.47 -1.2 1,513,161 - ydcV b1443 0.47 -2.0 1,513,229 + ydcV b1443 0.56 -0.9 1,513,229 + ydcV b1443 0.56 -0.9
Or see this region's nucleotide sequence