Experiment: Trimethoprim 0.0025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydcR and ydcS are separated by 244 nucleotides ydcS and ydcT are separated by 17 nucleotides
b1439: ydcR - fused predicted DNA-binding transcriptional regulator/predicted amino transferase (NCBI), at 1,508,027 to 1,509,433
ydcR
b1440: ydcS - predicted spermidine/putrescine transporter subunit (NCBI), at 1,509,678 to 1,510,823
ydcS
b1441: ydcT - predicted spermidine/putrescine transporter subunit (NCBI), at 1,510,841 to 1,511,854
ydcT
Position (kb)
1509
1510
1511 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1508.820 kb on + strand, within ydcR at 1508.820 kb on + strand, within ydcR at 1508.990 kb on - strand, within ydcR at 1509.170 kb on - strand, within ydcR at 1509.170 kb on - strand, within ydcR at 1509.236 kb on + strand, within ydcR at 1509.369 kb on + strand at 1509.375 kb on - strand at 1509.377 kb on - strand at 1509.377 kb on - strand at 1509.674 kb on - strand at 1509.693 kb on + strand at 1509.707 kb on + strand at 1509.718 kb on + strand at 1509.718 kb on + strand at 1509.733 kb on - strand at 1509.809 kb on + strand, within ydcS at 1509.834 kb on + strand, within ydcS at 1509.884 kb on + strand, within ydcS at 1510.004 kb on + strand, within ydcS at 1510.220 kb on + strand, within ydcS at 1510.227 kb on + strand, within ydcS at 1510.336 kb on - strand, within ydcS at 1510.336 kb on - strand, within ydcS at 1510.361 kb on + strand, within ydcS at 1510.390 kb on + strand, within ydcS at 1510.390 kb on + strand, within ydcS at 1510.392 kb on - strand, within ydcS at 1510.439 kb on - strand, within ydcS at 1510.661 kb on + strand, within ydcS at 1510.661 kb on + strand, within ydcS at 1510.763 kb on - strand at 1510.962 kb on - strand, within ydcT at 1511.021 kb on + strand, within ydcT at 1511.073 kb on + strand, within ydcT at 1511.302 kb on + strand, within ydcT at 1511.365 kb on - strand, within ydcT at 1511.428 kb on - strand, within ydcT at 1511.757 kb on - strand at 1511.782 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM remove 1,508,820 + ydcR b1439 0.56 -1.3 1,508,820 + ydcR b1439 0.56 -0.5 1,508,990 - ydcR b1439 0.68 +0.3 1,509,170 - ydcR b1439 0.81 +0.4 1,509,170 - ydcR b1439 0.81 -0.6 1,509,236 + ydcR b1439 0.86 -1.1 1,509,369 + +0.3 1,509,375 - +0.9 1,509,377 - +0.9 1,509,377 - +0.3 1,509,674 - +0.1 1,509,693 + -0.4 1,509,707 + +0.2 1,509,718 + +0.9 1,509,718 + +0.7 1,509,733 - -0.6 1,509,809 + ydcS b1440 0.11 -0.5 1,509,834 + ydcS b1440 0.14 -0.6 1,509,884 + ydcS b1440 0.18 -0.5 1,510,004 + ydcS b1440 0.28 +0.9 1,510,220 + ydcS b1440 0.47 -0.4 1,510,227 + ydcS b1440 0.48 -0.3 1,510,336 - ydcS b1440 0.57 -0.7 1,510,336 - ydcS b1440 0.57 +0.1 1,510,361 + ydcS b1440 0.60 -0.6 1,510,390 + ydcS b1440 0.62 -1.0 1,510,390 + ydcS b1440 0.62 -1.6 1,510,392 - ydcS b1440 0.62 +0.3 1,510,439 - ydcS b1440 0.66 +0.9 1,510,661 + ydcS b1440 0.86 -0.9 1,510,661 + ydcS b1440 0.86 -1.0 1,510,763 - +0.1 1,510,962 - ydcT b1441 0.12 +3.0 1,511,021 + ydcT b1441 0.18 -1.7 1,511,073 + ydcT b1441 0.23 -0.3 1,511,302 + ydcT b1441 0.45 -0.7 1,511,365 - ydcT b1441 0.52 -0.5 1,511,428 - ydcT b1441 0.58 +0.8 1,511,757 - -1.0 1,511,782 + -0.0
Or see this region's nucleotide sequence