Strain Fitness in Escherichia coli BW25113 around b1269

Experiment: Trimethoprim 0.0025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyciQ and rluB are separated by 210 nucleotidesrluB and btuR are separated by 39 nucleotidesbtuR and yciK overlap by 4 nucleotides b1268: yciQ - predicted inner membrane protein (NCBI), at 1,322,770 to 1,324,665 yciQ b1269: rluB - 23S rRNA pseudouridylate synthase (NCBI), at 1,324,876 to 1,325,751 rluB b1270: btuR - cob(I)yrinic acid a,c-diamide adenosyltransferase (NCBI), at 1,325,791 to 1,326,381 btuR b1271: yciK - short chain dehydrogenase (NCBI), at 1,326,378 to 1,327,136 yciK Position (kb) 1324 1325 1326Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1323.881 kb on - strand, within yciQat 1323.883 kb on + strand, within yciQat 1323.883 kb on + strand, within yciQat 1323.891 kb on - strand, within yciQat 1323.891 kb on - strand, within yciQat 1323.891 kb on - strand, within yciQat 1323.891 kb on - strand, within yciQat 1323.891 kb on - strand, within yciQat 1324.018 kb on + strand, within yciQat 1324.018 kb on + strand, within yciQat 1324.019 kb on - strand, within yciQat 1324.026 kb on - strand, within yciQat 1324.064 kb on - strand, within yciQat 1324.131 kb on + strand, within yciQat 1324.139 kb on - strand, within yciQat 1324.139 kb on - strand, within yciQat 1324.235 kb on + strand, within yciQat 1324.236 kb on + strand, within yciQat 1324.236 kb on + strand, within yciQat 1324.243 kb on - strand, within yciQat 1324.389 kb on + strand, within yciQat 1324.389 kb on + strand, within yciQat 1324.398 kb on - strand, within yciQat 1324.398 kb on - strand, within yciQat 1324.491 kb on + strandat 1324.821 kb on + strandat 1324.826 kb on + strandat 1324.829 kb on - strandat 1324.829 kb on - strandat 1325.097 kb on + strand, within rluBat 1325.110 kb on + strand, within rluBat 1325.179 kb on + strand, within rluBat 1325.179 kb on + strand, within rluBat 1325.635 kb on - strand, within rluBat 1325.636 kb on - strand, within rluBat 1325.636 kb on - strand, within rluBat 1325.814 kb on + strandat 1325.882 kb on - strand, within btuRat 1325.954 kb on - strand, within btuRat 1326.099 kb on - strand, within btuRat 1326.099 kb on - strand, within btuRat 1326.296 kb on - strand, within btuRat 1326.420 kb on + strandat 1326.420 kb on + strandat 1326.546 kb on + strand, within yciKat 1326.719 kb on + strand, within yciKat 1326.742 kb on - strand, within yciK

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM
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1,323,881 - yciQ b1268 0.59 -0.7
1,323,883 + yciQ b1268 0.59 -0.7
1,323,883 + yciQ b1268 0.59 -2.0
1,323,891 - yciQ b1268 0.59 +2.2
1,323,891 - yciQ b1268 0.59 +2.2
1,323,891 - yciQ b1268 0.59 +1.6
1,323,891 - yciQ b1268 0.59 +3.2
1,323,891 - yciQ b1268 0.59 +2.2
1,324,018 + yciQ b1268 0.66 +1.3
1,324,018 + yciQ b1268 0.66 -0.4
1,324,019 - yciQ b1268 0.66 +2.2
1,324,026 - yciQ b1268 0.66 +0.3
1,324,064 - yciQ b1268 0.68 +2.2
1,324,131 + yciQ b1268 0.72 +1.9
1,324,139 - yciQ b1268 0.72 +2.2
1,324,139 - yciQ b1268 0.72 +2.2
1,324,235 + yciQ b1268 0.77 -1.5
1,324,236 + yciQ b1268 0.77 -0.5
1,324,236 + yciQ b1268 0.77 +0.2
1,324,243 - yciQ b1268 0.78 +2.2
1,324,389 + yciQ b1268 0.85 -0.4
1,324,389 + yciQ b1268 0.85 +0.2
1,324,398 - yciQ b1268 0.86 -0.4
1,324,398 - yciQ b1268 0.86 -0.1
1,324,491 + -0.5
1,324,821 + +0.5
1,324,826 + -0.1
1,324,829 - +1.2
1,324,829 - +1.6
1,325,097 + rluB b1269 0.25 +0.2
1,325,110 + rluB b1269 0.27 -0.8
1,325,179 + rluB b1269 0.35 -0.3
1,325,179 + rluB b1269 0.35 -0.5
1,325,635 - rluB b1269 0.87 +0.2
1,325,636 - rluB b1269 0.87 -1.2
1,325,636 - rluB b1269 0.87 +0.6
1,325,814 + -1.7
1,325,882 - btuR b1270 0.15 +0.8
1,325,954 - btuR b1270 0.28 -0.2
1,326,099 - btuR b1270 0.52 -0.8
1,326,099 - btuR b1270 0.52 +0.7
1,326,296 - btuR b1270 0.85 -0.7
1,326,420 + -0.9
1,326,420 + -0.5
1,326,546 + yciK b1271 0.22 +0.4
1,326,719 + yciK b1271 0.45 -0.8
1,326,742 - yciK b1271 0.48 -2.7

Or see this region's nucleotide sequence