Strain Fitness in Escherichia coli BW25113 around b1257

Experiment: Trimethoprim 0.0025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntompW and yciE are separated by 59 nucleotidesyciE and yciF are separated by 45 nucleotidesyciF and yciG are separated by 85 nucleotides b1256: ompW - outer membrane protein W (NCBI), at 1,312,044 to 1,312,682 ompW b1257: yciE - hypothetical protein (NCBI), at 1,312,742 to 1,313,248 yciE b1258: yciF - hypothetical protein (NCBI), at 1,313,294 to 1,313,794 yciF b1259: yciG - orf, hypothetical protein (VIMSS), at 1,313,880 to 1,314,059 yciG Position (kb) 1312 1313 1314Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1311.747 kb on - strandat 1311.747 kb on - strandat 1311.747 kb on - strandat 1311.772 kb on - strandat 1311.858 kb on - strandat 1311.973 kb on + strandat 1311.973 kb on + strandat 1312.034 kb on - strandat 1312.096 kb on - strandat 1312.146 kb on + strand, within ompWat 1312.234 kb on + strand, within ompWat 1312.234 kb on + strand, within ompWat 1312.237 kb on - strand, within ompWat 1312.248 kb on + strand, within ompWat 1312.369 kb on + strand, within ompWat 1312.369 kb on + strand, within ompWat 1312.372 kb on - strand, within ompWat 1312.387 kb on + strand, within ompWat 1312.413 kb on + strand, within ompWat 1312.413 kb on + strand, within ompWat 1312.480 kb on - strand, within ompWat 1312.480 kb on - strand, within ompWat 1312.496 kb on + strand, within ompWat 1312.496 kb on + strand, within ompWat 1312.507 kb on - strand, within ompWat 1312.517 kb on + strand, within ompWat 1312.564 kb on + strand, within ompWat 1312.566 kb on + strand, within ompWat 1312.571 kb on + strand, within ompWat 1312.571 kb on + strand, within ompWat 1312.574 kb on + strand, within ompWat 1312.597 kb on + strand, within ompWat 1312.609 kb on + strand, within ompWat 1312.612 kb on + strand, within ompWat 1312.612 kb on + strand, within ompWat 1312.612 kb on + strand, within ompWat 1312.635 kb on + strandat 1312.638 kb on - strandat 1312.642 kb on + strandat 1312.642 kb on + strandat 1312.642 kb on + strandat 1312.644 kb on + strandat 1312.644 kb on + strandat 1312.653 kb on + strandat 1312.703 kb on + strandat 1312.704 kb on + strandat 1312.715 kb on + strandat 1312.725 kb on + strandat 1312.753 kb on + strandat 1312.909 kb on - strand, within yciEat 1313.347 kb on - strand, within yciFat 1313.409 kb on - strand, within yciFat 1313.479 kb on - strand, within yciFat 1313.499 kb on - strand, within yciFat 1313.642 kb on + strand, within yciFat 1313.939 kb on - strand, within yciGat 1313.996 kb on - strand, within yciG

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM
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1,311,747 - +0.5
1,311,747 - +2.1
1,311,747 - +0.3
1,311,772 - +0.1
1,311,858 - -0.8
1,311,973 + -0.3
1,311,973 + +0.3
1,312,034 - -0.7
1,312,096 - +1.0
1,312,146 + ompW b1256 0.16 -0.2
1,312,234 + ompW b1256 0.30 +0.0
1,312,234 + ompW b1256 0.30 +2.5
1,312,237 - ompW b1256 0.30 -2.8
1,312,248 + ompW b1256 0.32 +0.5
1,312,369 + ompW b1256 0.51 +0.4
1,312,369 + ompW b1256 0.51 -0.2
1,312,372 - ompW b1256 0.51 -0.8
1,312,387 + ompW b1256 0.54 -1.3
1,312,413 + ompW b1256 0.58 +0.3
1,312,413 + ompW b1256 0.58 -0.8
1,312,480 - ompW b1256 0.68 +0.8
1,312,480 - ompW b1256 0.68 +0.1
1,312,496 + ompW b1256 0.71 -0.1
1,312,496 + ompW b1256 0.71 -1.3
1,312,507 - ompW b1256 0.72 -0.2
1,312,517 + ompW b1256 0.74 +0.4
1,312,564 + ompW b1256 0.81 -0.3
1,312,566 + ompW b1256 0.82 -0.3
1,312,571 + ompW b1256 0.82 -1.4
1,312,571 + ompW b1256 0.82 -0.1
1,312,574 + ompW b1256 0.83 -0.4
1,312,597 + ompW b1256 0.87 +0.0
1,312,609 + ompW b1256 0.88 -0.3
1,312,612 + ompW b1256 0.89 -1.3
1,312,612 + ompW b1256 0.89 -1.8
1,312,612 + ompW b1256 0.89 -1.7
1,312,635 + -1.5
1,312,638 - +2.1
1,312,642 + -0.1
1,312,642 + -0.3
1,312,642 + -0.3
1,312,644 + -0.1
1,312,644 + -0.3
1,312,653 + +0.3
1,312,703 + +2.1
1,312,704 + -1.6
1,312,715 + +0.6
1,312,725 + +1.1
1,312,753 + +0.6
1,312,909 - yciE b1257 0.33 +1.1
1,313,347 - yciF b1258 0.11 +1.1
1,313,409 - yciF b1258 0.23 +1.6
1,313,479 - yciF b1258 0.37 +0.1
1,313,499 - yciF b1258 0.41 +2.1
1,313,642 + yciF b1258 0.69 +2.1
1,313,939 - yciG b1259 0.33 +0.6
1,313,996 - yciG b1259 0.64 +3.1

Or see this region's nucleotide sequence