Strain Fitness in Escherichia coli BW25113 around b1118

Experiment: Trimethoprim 0.0025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlolC and ycfV overlap by 8 nucleotidesycfV and lolE overlap by 1 nucleotideslolE and nagK are separated by 28 nucleotides b1116: lolC - outer membrane-specific lipoprotein transporter subunit (NCBI), at 1,174,650 to 1,175,849 lolC b1117: ycfV - putative ATP-binding component of a transport system (VIMSS), at 1,175,842 to 1,176,543 ycfV b1118: lolE - outer membrane-specific lipoprotein transporter subunit (NCBI), at 1,176,543 to 1,177,787 lolE b1119: nagK - N-acetyl-D-glucosamine kinase (NCBI), at 1,177,816 to 1,178,727 nagK Position (kb) 1176 1177 1178Strain fitness (log2 ratio) -1 0 1 2at 1176.533 kb on + strandat 1177.788 kb on + strandat 1177.791 kb on - strandat 1177.988 kb on - strand, within nagKat 1177.988 kb on - strand, within nagKat 1178.081 kb on + strand, within nagKat 1178.086 kb on + strand, within nagKat 1178.100 kb on + strand, within nagKat 1178.411 kb on - strand, within nagKat 1178.423 kb on + strand, within nagKat 1178.423 kb on + strand, within nagKat 1178.483 kb on + strand, within nagKat 1178.486 kb on - strand, within nagKat 1178.567 kb on - strand, within nagKat 1178.692 kb on - strandat 1178.693 kb on + strandat 1178.708 kb on + strandat 1178.708 kb on + strandat 1178.766 kb on + strandat 1178.766 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM
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1,176,533 + +0.7
1,177,788 + -0.2
1,177,791 - -0.1
1,177,988 - nagK b1119 0.19 +1.8
1,177,988 - nagK b1119 0.19 -0.2
1,178,081 + nagK b1119 0.29 -0.0
1,178,086 + nagK b1119 0.30 -0.5
1,178,100 + nagK b1119 0.31 -0.1
1,178,411 - nagK b1119 0.65 +0.6
1,178,423 + nagK b1119 0.67 -0.1
1,178,423 + nagK b1119 0.67 -1.5
1,178,483 + nagK b1119 0.73 -1.1
1,178,486 - nagK b1119 0.73 -0.4
1,178,567 - nagK b1119 0.82 -1.2
1,178,692 - -0.4
1,178,693 + +0.0
1,178,708 + -1.2
1,178,708 + -0.1
1,178,766 + -0.9
1,178,766 + +0.1

Or see this region's nucleotide sequence