Strain Fitness in Escherichia coli BW25113 around b1116

Experiment: Trimethoprim 0.0025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycfT and lolC are separated by 261 nucleotideslolC and ycfV overlap by 8 nucleotidesycfV and lolE overlap by 1 nucleotides b1115: ycfT - predicted inner membrane protein (NCBI), at 1,173,315 to 1,174,388 ycfT b1116: lolC - outer membrane-specific lipoprotein transporter subunit (NCBI), at 1,174,650 to 1,175,849 lolC b1117: ycfV - putative ATP-binding component of a transport system (VIMSS), at 1,175,842 to 1,176,543 ycfV b1118: lolE - outer membrane-specific lipoprotein transporter subunit (NCBI), at 1,176,543 to 1,177,787 lolE Position (kb) 1174 1175 1176Strain fitness (log2 ratio) -2 -1 0 1at 1173.710 kb on + strand, within ycfTat 1173.720 kb on + strand, within ycfTat 1173.720 kb on + strand, within ycfTat 1173.747 kb on + strand, within ycfTat 1173.757 kb on - strand, within ycfTat 1173.757 kb on - strand, within ycfTat 1173.767 kb on - strand, within ycfTat 1173.791 kb on + strand, within ycfTat 1173.791 kb on + strand, within ycfTat 1174.091 kb on - strand, within ycfTat 1174.091 kb on - strand, within ycfTat 1174.111 kb on + strand, within ycfTat 1174.176 kb on - strand, within ycfTat 1174.176 kb on - strand, within ycfTat 1174.267 kb on + strand, within ycfTat 1174.457 kb on + strandat 1176.533 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM
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1,173,710 + ycfT b1115 0.37 -1.1
1,173,720 + ycfT b1115 0.38 -0.2
1,173,720 + ycfT b1115 0.38 -0.5
1,173,747 + ycfT b1115 0.40 -0.8
1,173,757 - ycfT b1115 0.41 +0.6
1,173,757 - ycfT b1115 0.41 +0.9
1,173,767 - ycfT b1115 0.42 -0.2
1,173,791 + ycfT b1115 0.44 -1.0
1,173,791 + ycfT b1115 0.44 -1.2
1,174,091 - ycfT b1115 0.72 +0.5
1,174,091 - ycfT b1115 0.72 +0.3
1,174,111 + ycfT b1115 0.74 -0.5
1,174,176 - ycfT b1115 0.80 -2.2
1,174,176 - ycfT b1115 0.80 +0.5
1,174,267 + ycfT b1115 0.89 -0.1
1,174,457 + -1.9
1,176,533 + +0.7

Or see this region's nucleotide sequence