Experiment: Trimethoprim 0.0025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potF and potG are separated by 94 nucleotides potG and potH are separated by 9 nucleotides potH and potI overlap by 4 nucleotides
b0854: potF - putrescine transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 893,007 to 894,119
potF
b0855: potG - ATP-binding component of putrescine transport system (VIMSS), at 894,214 to 895,347
potG
b0856: potH - putrescine transporter subunit: membrane component of ABC superfamily (NCBI), at 895,357 to 896,310
potH
b0857: potI - putrescine transporter subunit: membrane component of ABC superfamily (NCBI), at 896,307 to 897,152
potI
Position (kb) 894
895
896 Strain fitness (log2 ratio) -2
-1
0
1
2 at 893.261 kb on + strand, within potF at 893.352 kb on - strand, within potF at 893.362 kb on - strand, within potF at 893.400 kb on + strand, within potF at 893.408 kb on - strand, within potF at 893.408 kb on - strand, within potF at 893.490 kb on - strand, within potF at 893.696 kb on - strand, within potF at 894.044 kb on - strand at 894.044 kb on - strand at 894.323 kb on + strand at 894.396 kb on + strand, within potG at 894.541 kb on - strand, within potG at 894.691 kb on - strand, within potG at 894.746 kb on - strand, within potG at 894.755 kb on + strand, within potG at 894.755 kb on + strand, within potG at 894.797 kb on - strand, within potG at 894.816 kb on + strand, within potG at 894.847 kb on - strand, within potG at 894.877 kb on + strand, within potG at 894.922 kb on + strand, within potG at 894.922 kb on + strand, within potG at 894.929 kb on - strand, within potG at 895.067 kb on + strand, within potG at 895.099 kb on - strand, within potG at 895.099 kb on - strand, within potG at 895.246 kb on + strand at 895.325 kb on + strand at 895.325 kb on + strand at 895.461 kb on + strand, within potH at 895.461 kb on + strand, within potH at 895.603 kb on - strand, within potH at 895.694 kb on + strand, within potH at 895.848 kb on + strand, within potH at 895.861 kb on - strand, within potH at 895.930 kb on + strand, within potH at 895.937 kb on - strand, within potH at 895.937 kb on - strand, within potH at 896.095 kb on - strand, within potH
Per-strain Table
Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM remove 893,261 + potF b0854 0.23 +0.3 893,352 - potF b0854 0.31 +0.8 893,362 - potF b0854 0.32 -0.3 893,400 + potF b0854 0.35 +0.3 893,408 - potF b0854 0.36 -0.8 893,408 - potF b0854 0.36 +1.0 893,490 - potF b0854 0.43 -0.6 893,696 - potF b0854 0.62 +0.1 894,044 - -1.1 894,044 - -0.8 894,323 + -0.3 894,396 + potG b0855 0.16 -0.4 894,541 - potG b0855 0.29 -0.7 894,691 - potG b0855 0.42 -2.5 894,746 - potG b0855 0.47 +0.1 894,755 + potG b0855 0.48 +0.9 894,755 + potG b0855 0.48 -1.1 894,797 - potG b0855 0.51 +2.6 894,816 + potG b0855 0.53 -0.7 894,847 - potG b0855 0.56 -0.8 894,877 + potG b0855 0.58 -0.2 894,922 + potG b0855 0.62 +0.3 894,922 + potG b0855 0.62 +0.5 894,929 - potG b0855 0.63 -0.6 895,067 + potG b0855 0.75 +0.4 895,099 - potG b0855 0.78 +0.0 895,099 - potG b0855 0.78 -0.3 895,246 + -0.2 895,325 + -1.4 895,325 + -0.1 895,461 + potH b0856 0.11 +0.0 895,461 + potH b0856 0.11 -0.3 895,603 - potH b0856 0.26 -0.1 895,694 + potH b0856 0.35 +1.3 895,848 + potH b0856 0.51 +1.4 895,861 - potH b0856 0.53 -0.6 895,930 + potH b0856 0.60 -0.8 895,937 - potH b0856 0.61 -0.6 895,937 - potH b0856 0.61 +1.2 896,095 - potH b0856 0.77 -1.1
Or see this region's nucleotide sequence