Experiment: Trimethoprim 0.0025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fur and uof overlap by 8 nucleotides uof and fldA are separated by 209 nucleotides fldA and ybfE are separated by 139 nucleotides ybfE and ybfF are separated by 139 nucleotides
b0683: fur - ferric uptake regulator (NCBI), at 709,423 to 709,869
fur
b4637: uof - ryhB-regulated fur leader peptide (RefSeq), at 709,862 to 709,948
uof
b0684: fldA - flavodoxin 1 (NCBI), at 710,158 to 710,688
fldA
b0685: ybfE - orf, hypothetical protein (VIMSS), at 710,828 to 711,121
ybfE
b0686: ybfF - hypothetical protein (NCBI), at 711,261 to 712,025
ybfF
Position (kb) 710
711
712 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8 at 709.861 kb on - strand at 710.067 kb on - strand at 710.080 kb on + strand at 710.148 kb on - strand at 710.148 kb on - strand at 710.163 kb on - strand at 710.751 kb on + strand at 710.780 kb on - strand at 710.780 kb on - strand at 710.787 kb on + strand at 710.798 kb on + strand at 710.805 kb on - strand at 710.805 kb on - strand at 710.807 kb on + strand at 710.901 kb on + strand, within ybfE at 710.924 kb on + strand, within ybfE at 710.924 kb on + strand, within ybfE at 711.159 kb on - strand at 711.400 kb on - strand, within ybfF at 711.569 kb on + strand, within ybfF at 711.668 kb on - strand, within ybfF at 711.685 kb on + strand, within ybfF at 711.728 kb on - strand, within ybfF at 711.730 kb on - strand, within ybfF at 711.730 kb on - strand, within ybfF at 711.803 kb on - strand, within ybfF at 711.803 kb on - strand, within ybfF at 711.900 kb on - strand, within ybfF at 711.923 kb on + strand, within ybfF at 711.923 kb on + strand, within ybfF at 711.933 kb on + strand, within ybfF at 712.060 kb on + strand at 712.077 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM remove 709,861 - -1.0 710,067 - -0.2 710,080 + +0.7 710,148 - +0.6 710,148 - +0.2 710,163 - +0.1 710,751 + -1.9 710,780 - +0.8 710,780 - +0.0 710,787 + +5.3 710,798 + +4.4 710,805 - -0.2 710,805 - -0.7 710,807 + +4.4 710,901 + ybfE b0685 0.25 +8.6 710,924 + ybfE b0685 0.33 +7.5 710,924 + ybfE b0685 0.33 +7.5 711,159 - +1.3 711,400 - ybfF b0686 0.18 -0.3 711,569 + ybfF b0686 0.40 +0.1 711,668 - ybfF b0686 0.53 +0.8 711,685 + ybfF b0686 0.55 +1.8 711,728 - ybfF b0686 0.61 +0.4 711,730 - ybfF b0686 0.61 -0.3 711,730 - ybfF b0686 0.61 -0.3 711,803 - ybfF b0686 0.71 +0.4 711,803 - ybfF b0686 0.71 -1.3 711,900 - ybfF b0686 0.84 +0.4 711,923 + ybfF b0686 0.87 +0.8 711,923 + ybfF b0686 0.87 +0.6 711,933 + ybfF b0686 0.88 +1.0 712,060 + +1.0 712,077 + -2.5
Or see this region's nucleotide sequence