Experiment: Trimethoprim 0.0025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt degP and yaeG are separated by 154 nucleotides yaeG and yaeH are separated by 88 nucleotides yaeH and yaeI are separated by 161 nucleotides
b0161: degP - serine endoprotease (protease Do), membrane-associated (NCBI), at 180,884 to 182,308
degP
b0162: yaeG - orf, hypothetical protein (VIMSS), at 182,463 to 183,620
yaeG
b0163: yaeH - hypothetical protein (NCBI), at 183,709 to 184,095
yaeH
b0164: yaeI - orf, hypothetical protein (VIMSS), at 184,257 to 185,069
yaeI
Position (kb) 182
183
184 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 181.552 kb on - strand, within degP at 181.615 kb on - strand, within degP at 181.638 kb on - strand, within degP at 181.638 kb on - strand, within degP at 181.658 kb on + strand, within degP at 181.680 kb on + strand, within degP at 181.688 kb on - strand, within degP at 181.758 kb on + strand, within degP at 181.758 kb on + strand, within degP at 181.782 kb on + strand, within degP at 181.867 kb on - strand, within degP at 181.867 kb on - strand, within degP at 181.903 kb on - strand, within degP at 181.937 kb on + strand, within degP at 181.942 kb on + strand, within degP at 181.984 kb on + strand, within degP at 181.995 kb on + strand, within degP at 182.013 kb on + strand, within degP at 182.013 kb on + strand, within degP at 182.048 kb on + strand, within degP at 182.050 kb on + strand, within degP at 182.060 kb on + strand, within degP at 182.060 kb on + strand, within degP at 182.070 kb on + strand, within degP at 182.070 kb on + strand, within degP at 182.099 kb on + strand, within degP at 182.099 kb on + strand, within degP at 182.125 kb on - strand, within degP at 182.125 kb on - strand, within degP at 182.224 kb on + strand at 182.285 kb on + strand at 182.363 kb on - strand at 182.363 kb on - strand at 182.381 kb on - strand at 182.393 kb on + strand at 182.430 kb on + strand at 182.435 kb on + strand at 182.443 kb on - strand at 182.453 kb on - strand at 182.459 kb on + strand at 182.509 kb on + strand at 182.634 kb on - strand, within yaeG at 182.741 kb on + strand, within yaeG at 182.891 kb on + strand, within yaeG at 182.891 kb on + strand, within yaeG at 182.896 kb on + strand, within yaeG at 183.060 kb on - strand, within yaeG at 183.113 kb on - strand, within yaeG at 183.113 kb on - strand, within yaeG at 183.181 kb on + strand, within yaeG at 183.229 kb on + strand, within yaeG at 183.237 kb on - strand, within yaeG at 183.275 kb on + strand, within yaeG at 183.337 kb on - strand, within yaeG at 183.510 kb on + strand at 183.518 kb on - strand at 183.518 kb on - strand at 183.543 kb on - strand at 183.584 kb on + strand at 183.584 kb on + strand at 183.643 kb on + strand at 183.663 kb on + strand at 183.663 kb on + strand at 183.672 kb on + strand at 183.904 kb on - strand, within yaeH at 183.924 kb on - strand, within yaeH at 183.929 kb on + strand, within yaeH at 183.937 kb on - strand, within yaeH at 183.943 kb on + strand, within yaeH at 184.035 kb on + strand, within yaeH at 184.100 kb on - strand at 184.130 kb on + strand at 184.193 kb on - strand at 184.283 kb on - strand at 184.283 kb on - strand at 184.312 kb on + strand at 184.351 kb on - strand, within yaeI at 184.439 kb on + strand, within yaeI at 184.455 kb on + strand, within yaeI at 184.579 kb on + strand, within yaeI
Per-strain Table
Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM remove 181,552 - degP b0161 0.47 -1.8 181,615 - degP b0161 0.51 -0.9 181,638 - degP b0161 0.53 +1.7 181,638 - degP b0161 0.53 +0.1 181,658 + degP b0161 0.54 -1.1 181,680 + degP b0161 0.56 -1.8 181,688 - degP b0161 0.56 -0.8 181,758 + degP b0161 0.61 -1.6 181,758 + degP b0161 0.61 +3.7 181,782 + degP b0161 0.63 -1.6 181,867 - degP b0161 0.69 -1.0 181,867 - degP b0161 0.69 +0.5 181,903 - degP b0161 0.72 +0.3 181,937 + degP b0161 0.74 +0.4 181,942 + degP b0161 0.74 +0.7 181,984 + degP b0161 0.77 -0.4 181,995 + degP b0161 0.78 -0.4 182,013 + degP b0161 0.79 -1.0 182,013 + degP b0161 0.79 +0.4 182,048 + degP b0161 0.82 +0.4 182,050 + degP b0161 0.82 -0.1 182,060 + degP b0161 0.83 +0.3 182,060 + degP b0161 0.83 -0.3 182,070 + degP b0161 0.83 +0.6 182,070 + degP b0161 0.83 +0.3 182,099 + degP b0161 0.85 +0.3 182,099 + degP b0161 0.85 -1.0 182,125 - degP b0161 0.87 -1.3 182,125 - degP b0161 0.87 -0.9 182,224 + +0.3 182,285 + +0.7 182,363 - -1.0 182,363 - -0.7 182,381 - +0.3 182,393 + -0.2 182,430 + -0.4 182,435 + -0.7 182,443 - -0.4 182,453 - +0.4 182,459 + +0.4 182,509 + -0.9 182,634 - yaeG b0162 0.15 -0.8 182,741 + yaeG b0162 0.24 -0.4 182,891 + yaeG b0162 0.37 -1.3 182,891 + yaeG b0162 0.37 -0.8 182,896 + yaeG b0162 0.37 +0.3 183,060 - yaeG b0162 0.52 -0.4 183,113 - yaeG b0162 0.56 -0.3 183,113 - yaeG b0162 0.56 -0.5 183,181 + yaeG b0162 0.62 -0.8 183,229 + yaeG b0162 0.66 -0.7 183,237 - yaeG b0162 0.67 -1.2 183,275 + yaeG b0162 0.70 -0.2 183,337 - yaeG b0162 0.75 -2.0 183,510 + +0.0 183,518 - -1.1 183,518 - -0.1 183,543 - -0.7 183,584 + -0.4 183,584 + -0.3 183,643 + +0.4 183,663 + -0.2 183,663 + -0.8 183,672 + -0.2 183,904 - yaeH b0163 0.50 -0.4 183,924 - yaeH b0163 0.56 +0.3 183,929 + yaeH b0163 0.57 +0.4 183,937 - yaeH b0163 0.59 +0.6 183,943 + yaeH b0163 0.60 -0.5 184,035 + yaeH b0163 0.84 -0.7 184,100 - +0.5 184,130 + -0.5 184,193 - +0.4 184,283 - +0.2 184,283 - -1.0 184,312 + +0.7 184,351 - yaeI b0164 0.12 -1.1 184,439 + yaeI b0164 0.22 +0.9 184,455 + yaeI b0164 0.24 +0.3 184,579 + yaeI b0164 0.40 -0.8
Or see this region's nucleotide sequence