Strain Fitness in Escherichia coli BW25113 around b0051

Experiment: Trimethoprim 0.0025 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Trimethoprim 0.0025 mM
50,717	-	apaH	b0049	0.40	-1.0
50,785	-	apaH	b0049	0.48	-2.4
50,791	+	apaH	b0049	0.49	+2.7
50,802	+	apaH	b0049	0.50	+2.4
50,829	-	apaH	b0049	0.53	+1.0
50,859	-	apaH	b0049	0.57	-1.5
50,877	+	apaH	b0049	0.59	+2.1
50,877	+	apaH	b0049	0.59	+0.1
51,025	+	apaH	b0049	0.77	+0.8
51,109	-	apaH	b0049	0.86	-0.4
51,177	+				-1.1
51,177	+				+1.7
51,185	-				-0.1
51,190	+				+2.0
51,190	+				+1.6
51,240	+				+2.0
51,240	+				+1.5
51,311	+	apaG	b0050	0.22	+0.6
51,311	+	apaG	b0050	0.22	+0.5
51,322	-	apaG	b0050	0.25	-0.9
51,326	-	apaG	b0050	0.26	-3.5
51,334	-	apaG	b0050	0.28	-1.9
51,336	-	apaG	b0050	0.28	-1.0
51,353	+	apaG	b0050	0.33	+1.4
51,353	+	apaG	b0050	0.33	+1.2
51,353	-	apaG	b0050	0.33	-2.6
51,364	-	apaG	b0050	0.36	-1.3
51,372	-	apaG	b0050	0.38	-1.0
51,415	+	apaG	b0050	0.49	+1.3
51,423	-	apaG	b0050	0.51	-1.9
51,466	+	apaG	b0050	0.63	+1.0
51,472	+	apaG	b0050	0.64	+1.6
51,512	-	apaG	b0050	0.75	-0.9
51,514	-	apaG	b0050	0.75	-2.0
51,520	-	apaG	b0050	0.77	-1.1
51,524	+	apaG	b0050	0.78	+2.3
51,550	-	apaG	b0050	0.85	-0.8
51,560	+	apaG	b0050	0.88	+2.5
51,567	-	apaG	b0050	0.89	-0.9
51,567	-	apaG	b0050	0.89	-0.5
51,576	+				+1.5
51,576	+				+1.8
51,586	-				-1.4
51,613	+				+2.4
51,614	-				-1.3
51,615	+				+2.0
51,615	+				+2.6
51,673	-				+1.5
51,703	+	ksgA	b0051	0.11	+2.9
51,703	+	ksgA	b0051	0.11	+4.2
51,812	-	ksgA	b0051	0.25	+1.4
51,836	+	ksgA	b0051	0.28	+4.0
51,891	-	ksgA	b0051	0.34	+3.0
51,891	-	ksgA	b0051	0.34	+1.7
51,904	+	ksgA	b0051	0.36	+4.5
51,906	+	ksgA	b0051	0.36	+4.4
51,925	-	ksgA	b0051	0.38	-0.3
52,040	+	ksgA	b0051	0.52	+4.3
52,040	+	ksgA	b0051	0.52	+4.4
52,040	+	ksgA	b0051	0.52	+4.3
52,042	+	ksgA	b0051	0.53	+4.6
52,042	+	ksgA	b0051	0.53	+4.1
52,042	+	ksgA	b0051	0.53	+4.1
52,042	-	ksgA	b0051	0.53	+3.3
52,042	-	ksgA	b0051	0.53	+1.0
52,045	+	ksgA	b0051	0.53	+3.3
52,062	+	ksgA	b0051	0.55	+4.8
52,062	+				+3.9
52,070	-	ksgA	b0051	0.56	+0.2
52,072	+	ksgA	b0051	0.56	+3.9
52,089	-	ksgA	b0051	0.58	+1.3
52,276	+	ksgA	b0051	0.81	+4.3
52,276	+	ksgA	b0051	0.81	+4.0
52,284	+	ksgA	b0051	0.82	+4.2
52,338	+	ksgA	b0051	0.89	+5.5
52,338	+	ksgA	b0051	0.89	+3.5
52,343	-	ksgA	b0051	0.89	+2.7
52,348	-				+1.9
52,348	-				+2.5
52,348	-				+0.8
52,354	+				+3.7
52,420	-				+3.8
52,490	+				+2.6
52,490	+				+3.6
52,502	+				+3.3
52,502	+				+5.7
52,518	-				-0.4
52,518	-				-0.6
52,626	+	pdxA	b0052	0.20	-0.0
52,688	-	pdxA	b0052	0.26	-1.0
52,735	+	pdxA	b0052	0.31	+0.3
52,735	+	pdxA	b0052	0.31	+0.8
52,740	-	pdxA	b0052	0.32	-1.0
52,766	-	pdxA	b0052	0.34	-1.2
52,780	-	pdxA	b0052	0.36	+0.2
52,797	-	pdxA	b0052	0.37	-0.6
52,879	-	pdxA	b0052	0.46	-0.6
52,914	+	pdxA	b0052	0.49	+0.1
52,982	-	pdxA	b0052	0.56	+0.7
52,982	-	pdxA	b0052	0.56	-1.7
52,983	+	pdxA	b0052	0.56	+0.7
53,061	-	pdxA	b0052	0.64	-1.9
53,062	-	pdxA	b0052	0.64	-1.0
53,062	-				+0.1
53,064	+	pdxA	b0052	0.64	+1.0
53,067	-	pdxA	b0052	0.65	+0.0
53,163	-	pdxA	b0052	0.74	-0.5
53,163	-	pdxA	b0052	0.74	-1.2
53,181	-	pdxA	b0052	0.76	-2.1
53,181	-	pdxA	b0052	0.76	-0.7
53,202	-	pdxA	b0052	0.78	-2.0
53,221	+	pdxA	b0052	0.80	+1.3
53,221	+	pdxA	b0052	0.80	+1.0
53,226	-	pdxA	b0052	0.81	-0.3
53,246	-	pdxA	b0052	0.83	-0.5
53,291	+	pdxA	b0052	0.87	+0.5
53,328	+				+1.5
53,328	+				+0.8
53,338	+				+1.1
53,338	+				+0.8
53,338	+				+1.8
53,338	+				+0.8
53,346	-				+0.2
53,346	-				+1.3
53,346	-				+0.4