Strain Fitness in Escherichia coli BW25113 around b0048

Experiment: Trimethoprim 0.0025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntkefC and folA are separated by 191 nucleotidesfolA and apaH are separated by 77 nucleotidesapaH and apaG are separated by 6 nucleotides b0047: kefC - glutathione-regulated potassium-efflux system protein (NCBI), at 47,769 to 49,631 kefC b0048: folA - dihydrofolate reductase (NCBI), at 49,823 to 50,302 folA b0049: apaH - diadenosinetetraphosphatase (NCBI), at 50,380 to 51,222 apaH b0050: apaG - hypothetical protein (NCBI), at 51,229 to 51,606 apaG Position (kb) 49 50 51Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6 7 8 9 10at 48.907 kb on + strand, within kefCat 48.958 kb on + strand, within kefCat 48.968 kb on - strand, within kefCat 49.068 kb on - strand, within kefCat 49.068 kb on - strand, within kefCat 49.149 kb on - strand, within kefCat 49.149 kb on - strand, within kefCat 49.202 kb on + strand, within kefCat 49.281 kb on + strand, within kefCat 49.289 kb on - strand, within kefCat 49.289 kb on - strand, within kefCat 49.334 kb on + strand, within kefCat 49.334 kb on + strand, within kefCat 49.356 kb on - strand, within kefCat 49.356 kb on - strand, within kefCat 49.369 kb on + strand, within kefCat 49.369 kb on + strand, within kefCat 49.409 kb on - strand, within kefCat 49.431 kb on + strand, within kefCat 49.487 kb on - strandat 49.493 kb on - strandat 49.709 kb on + strandat 50.361 kb on + strandat 50.361 kb on + strandat 50.370 kb on - strandat 50.376 kb on + strandat 50.461 kb on + strandat 50.469 kb on - strand, within apaHat 50.501 kb on + strand, within apaHat 50.507 kb on - strand, within apaHat 50.568 kb on - strand, within apaHat 50.717 kb on - strand, within apaHat 50.785 kb on - strand, within apaHat 50.791 kb on + strand, within apaHat 50.802 kb on + strand, within apaHat 50.829 kb on - strand, within apaHat 50.859 kb on - strand, within apaHat 50.877 kb on + strand, within apaHat 50.877 kb on + strand, within apaHat 51.025 kb on + strand, within apaHat 51.109 kb on - strand, within apaHat 51.177 kb on + strandat 51.177 kb on + strandat 51.185 kb on - strandat 51.190 kb on + strandat 51.190 kb on + strandat 51.240 kb on + strandat 51.240 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM
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48,907 + kefC b0047 0.61 -1.3
48,958 + kefC b0047 0.64 -1.4
48,968 - kefC b0047 0.64 +0.1
49,068 - kefC b0047 0.70 +1.8
49,068 - kefC b0047 0.70 +0.4
49,149 - kefC b0047 0.74 -0.0
49,149 - kefC b0047 0.74 +0.9
49,202 + kefC b0047 0.77 -2.2
49,281 + kefC b0047 0.81 -0.3
49,289 - kefC b0047 0.82 -0.8
49,289 - kefC b0047 0.82 +0.1
49,334 + kefC b0047 0.84 -0.9
49,334 + kefC b0047 0.84 -0.2
49,356 - kefC b0047 0.85 +0.9
49,356 - kefC b0047 0.85 +0.5
49,369 + kefC b0047 0.86 -1.6
49,369 + kefC b0047 0.86 -0.3
49,409 - kefC b0047 0.88 +1.3
49,431 + kefC b0047 0.89 -1.1
49,487 - +1.7
49,493 - +1.1
49,709 + +9.8
50,361 + +0.4
50,361 + +0.9
50,370 - -2.1
50,376 + +1.6
50,461 + +2.4
50,469 - apaH b0049 0.11 +0.8
50,501 + apaH b0049 0.14 +3.0
50,507 - apaH b0049 0.15 +0.5
50,568 - apaH b0049 0.22 -0.9
50,717 - apaH b0049 0.40 -1.0
50,785 - apaH b0049 0.48 -2.4
50,791 + apaH b0049 0.49 +2.7
50,802 + apaH b0049 0.50 +2.4
50,829 - apaH b0049 0.53 +1.0
50,859 - apaH b0049 0.57 -1.5
50,877 + apaH b0049 0.59 +2.1
50,877 + apaH b0049 0.59 +0.1
51,025 + apaH b0049 0.77 +0.8
51,109 - apaH b0049 0.86 -0.4
51,177 + -1.1
51,177 + +1.7
51,185 - -0.1
51,190 + +2.0
51,190 + +1.6
51,240 + +2.0
51,240 + +1.5

Or see this region's nucleotide sequence