Experiment: Trimethoprim 0.00125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yidC and trmE are separated by 105 nucleotides trmE and tnaC are separated by 242 nucleotides tnaC and tnaA are separated by 220 nucleotides
b3705: yidC - inner membrane protein translocase component YidC (NCBI), at 3,883,099 to 3,884,745
yidC
b3706: trmE - tRNA modification GTPase (NCBI), at 3,884,851 to 3,886,215
trmE
b3707: tnaC - tryptophanase leader peptide (NCBI), at 3,886,458 to 3,886,532
tnaC
b3708: tnaA - tryptophanase (VIMSS), at 3,886,753 to 3,888,168
tnaA
Position (kb)
3884
3885
3886
3887 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3884.716 kb on + strand at 3884.754 kb on + strand at 3884.777 kb on + strand at 3884.777 kb on + strand at 3884.817 kb on - strand at 3884.817 kb on - strand at 3884.817 kb on - strand at 3884.855 kb on - strand at 3884.855 kb on - strand at 3884.869 kb on + strand at 3884.869 kb on + strand at 3884.869 kb on + strand at 3884.869 kb on + strand at 3884.879 kb on + strand at 3884.883 kb on - strand at 3884.888 kb on - strand at 3884.897 kb on - strand at 3884.903 kb on + strand at 3884.937 kb on - strand at 3884.961 kb on + strand at 3884.961 kb on + strand at 3885.010 kb on + strand, within trmE at 3885.014 kb on + strand, within trmE at 3885.041 kb on - strand, within trmE at 3885.051 kb on - strand, within trmE at 3885.066 kb on - strand, within trmE at 3885.091 kb on + strand, within trmE at 3885.099 kb on - strand, within trmE at 3885.129 kb on + strand, within trmE at 3885.181 kb on - strand, within trmE at 3885.181 kb on - strand, within trmE at 3885.342 kb on - strand, within trmE at 3885.342 kb on - strand, within trmE at 3885.342 kb on - strand, within trmE at 3885.400 kb on - strand, within trmE at 3885.461 kb on + strand, within trmE at 3885.480 kb on - strand, within trmE at 3885.482 kb on - strand, within trmE at 3885.490 kb on + strand, within trmE at 3885.522 kb on + strand, within trmE at 3885.689 kb on + strand, within trmE at 3885.758 kb on - strand, within trmE at 3885.758 kb on - strand, within trmE at 3885.793 kb on - strand, within trmE at 3885.887 kb on + strand, within trmE at 3885.912 kb on + strand, within trmE at 3885.929 kb on + strand, within trmE at 3885.929 kb on + strand, within trmE at 3885.944 kb on + strand, within trmE at 3885.962 kb on + strand, within trmE at 3885.962 kb on + strand, within trmE at 3885.987 kb on - strand, within trmE at 3886.006 kb on + strand, within trmE at 3886.033 kb on - strand, within trmE at 3886.033 kb on - strand, within trmE at 3886.072 kb on + strand, within trmE at 3886.086 kb on + strand at 3886.086 kb on - strand at 3886.094 kb on + strand at 3886.197 kb on + strand at 3886.200 kb on + strand at 3886.200 kb on + strand at 3886.200 kb on + strand at 3886.202 kb on + strand at 3886.207 kb on + strand at 3886.210 kb on + strand at 3886.233 kb on + strand at 3886.233 kb on + strand at 3886.237 kb on - strand at 3886.243 kb on + strand at 3886.257 kb on + strand at 3886.258 kb on + strand at 3886.258 kb on + strand at 3886.258 kb on + strand at 3886.263 kb on + strand at 3886.268 kb on + strand at 3886.273 kb on + strand at 3886.273 kb on + strand at 3886.373 kb on - strand at 3886.416 kb on + strand at 3886.419 kb on + strand at 3886.423 kb on + strand at 3886.440 kb on + strand at 3886.440 kb on + strand at 3886.471 kb on + strand, within tnaC at 3886.526 kb on + strand at 3886.537 kb on + strand at 3886.539 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.541 kb on + strand at 3886.547 kb on + strand at 3886.547 kb on + strand at 3886.547 kb on + strand at 3886.549 kb on + strand at 3886.552 kb on + strand at 3886.572 kb on + strand at 3886.573 kb on + strand at 3886.599 kb on + strand at 3886.662 kb on + strand at 3886.670 kb on + strand at 3886.713 kb on + strand at 3886.772 kb on + strand at 3886.780 kb on - strand at 3886.780 kb on - strand at 3886.780 kb on - strand at 3886.780 kb on - strand at 3886.781 kb on - strand at 3886.781 kb on - strand at 3886.788 kb on + strand at 3886.804 kb on - strand at 3886.810 kb on - strand at 3886.810 kb on - strand at 3886.813 kb on - strand at 3886.832 kb on - strand at 3886.837 kb on - strand at 3886.837 kb on - strand at 3886.846 kb on + strand at 3886.853 kb on - strand at 3886.853 kb on - strand at 3886.868 kb on - strand at 3886.868 kb on - strand at 3886.874 kb on - strand at 3886.874 kb on - strand at 3886.875 kb on + strand at 3886.875 kb on + strand at 3886.875 kb on + strand at 3886.875 kb on + strand at 3886.875 kb on - strand at 3886.878 kb on - strand at 3886.878 kb on - strand at 3886.914 kb on - strand, within tnaA at 3886.924 kb on - strand, within tnaA at 3886.924 kb on - strand, within tnaA at 3886.924 kb on - strand, within tnaA at 3886.931 kb on + strand, within tnaA at 3886.935 kb on - strand, within tnaA at 3886.935 kb on - strand, within tnaA at 3886.938 kb on - strand, within tnaA at 3886.938 kb on - strand, within tnaA at 3886.938 kb on - strand, within tnaA at 3886.939 kb on - strand, within tnaA at 3886.939 kb on - strand, within tnaA at 3886.943 kb on + strand, within tnaA at 3886.943 kb on - strand, within tnaA at 3886.946 kb on - strand, within tnaA at 3886.946 kb on - strand at 3886.952 kb on - strand, within tnaA at 3886.952 kb on - strand, within tnaA at 3886.969 kb on + strand, within tnaA at 3886.972 kb on - strand, within tnaA at 3886.977 kb on - strand, within tnaA at 3886.984 kb on + strand, within tnaA at 3886.988 kb on + strand, within tnaA at 3886.990 kb on - strand, within tnaA at 3886.990 kb on - strand, within tnaA at 3886.994 kb on - strand, within tnaA at 3886.994 kb on - strand, within tnaA at 3886.999 kb on + strand, within tnaA at 3887.032 kb on + strand, within tnaA at 3887.039 kb on + strand, within tnaA at 3887.039 kb on - strand, within tnaA at 3887.039 kb on - strand, within tnaA at 3887.039 kb on - strand, within tnaA at 3887.039 kb on - strand, within tnaA at 3887.040 kb on - strand, within tnaA at 3887.040 kb on - strand, within tnaA at 3887.045 kb on + strand, within tnaA at 3887.066 kb on - strand, within tnaA at 3887.076 kb on - strand, within tnaA at 3887.083 kb on - strand, within tnaA at 3887.083 kb on - strand, within tnaA at 3887.116 kb on + strand, within tnaA at 3887.116 kb on + strand, within tnaA at 3887.175 kb on - strand, within tnaA at 3887.190 kb on + strand, within tnaA at 3887.190 kb on + strand, within tnaA at 3887.192 kb on + strand, within tnaA at 3887.192 kb on + strand, within tnaA at 3887.192 kb on + strand, within tnaA at 3887.192 kb on - strand, within tnaA at 3887.197 kb on + strand, within tnaA at 3887.197 kb on + strand, within tnaA at 3887.198 kb on - strand, within tnaA at 3887.200 kb on + strand, within tnaA
Per-strain Table
Position Strand Gene LocusTag Fraction Trimethoprim 0.00125 mM remove 3,884,716 + -2.1 3,884,754 + +0.2 3,884,777 + +0.3 3,884,777 + -0.7 3,884,817 - -0.7 3,884,817 - +0.1 3,884,817 - -0.4 3,884,855 - -0.8 3,884,855 - -0.1 3,884,869 + +1.6 3,884,869 + +0.4 3,884,869 + +1.6 3,884,869 + +1.6 3,884,879 + +1.3 3,884,883 - +1.0 3,884,888 - +1.0 3,884,897 - -0.7 3,884,903 + -0.0 3,884,937 - +0.6 3,884,961 + +0.6 3,884,961 + +1.0 3,885,010 + trmE b3706 0.12 +0.6 3,885,014 + trmE b3706 0.12 -1.0 3,885,041 - trmE b3706 0.14 +2.2 3,885,051 - trmE b3706 0.15 +1.9 3,885,066 - trmE b3706 0.16 +1.6 3,885,091 + trmE b3706 0.18 +2.3 3,885,099 - trmE b3706 0.18 +1.0 3,885,129 + trmE b3706 0.20 +3.2 3,885,181 - trmE b3706 0.24 -1.2 3,885,181 - trmE b3706 0.24 +0.6 3,885,342 - trmE b3706 0.36 +0.8 3,885,342 - trmE b3706 0.36 -0.4 3,885,342 - trmE b3706 0.36 +2.6 3,885,400 - trmE b3706 0.40 +0.7 3,885,461 + trmE b3706 0.45 -0.7 3,885,480 - trmE b3706 0.46 +1.2 3,885,482 - trmE b3706 0.46 +0.3 3,885,490 + trmE b3706 0.47 +1.6 3,885,522 + trmE b3706 0.49 -0.2 3,885,689 + trmE b3706 0.61 +1.6 3,885,758 - trmE b3706 0.66 +1.3 3,885,758 - trmE b3706 0.66 +2.0 3,885,793 - trmE b3706 0.69 +0.6 3,885,887 + trmE b3706 0.76 +0.6 3,885,912 + trmE b3706 0.78 +2.0 3,885,929 + trmE b3706 0.79 +1.6 3,885,929 + trmE b3706 0.79 -0.0 3,885,944 + trmE b3706 0.80 +1.8 3,885,962 + trmE b3706 0.81 +1.0 3,885,962 + trmE b3706 0.81 -0.3 3,885,987 - trmE b3706 0.83 +1.6 3,886,006 + trmE b3706 0.85 +1.0 3,886,033 - trmE b3706 0.87 -0.0 3,886,033 - trmE b3706 0.87 +1.6 3,886,072 + trmE b3706 0.89 +0.8 3,886,086 + -1.2 3,886,086 - +1.1 3,886,094 + -0.0 3,886,197 + +1.4 3,886,200 + +1.6 3,886,200 + +0.6 3,886,200 + +0.6 3,886,202 + +0.6 3,886,207 + +0.6 3,886,210 + -0.4 3,886,233 + +0.7 3,886,233 + -0.1 3,886,237 - -0.4 3,886,243 + +0.6 3,886,257 + +0.1 3,886,258 + -0.2 3,886,258 + -1.0 3,886,258 + -1.1 3,886,263 + +1.2 3,886,268 + -0.2 3,886,273 + +2.0 3,886,273 + +1.6 3,886,373 - +0.6 3,886,416 + +2.2 3,886,419 + +0.1 3,886,423 + -0.0 3,886,440 + -1.1 3,886,440 + -0.6 3,886,471 + tnaC b3707 0.17 -0.4 3,886,526 + -0.7 3,886,537 + -0.2 3,886,539 + +1.4 3,886,541 + -0.7 3,886,541 + -1.1 3,886,541 + -0.7 3,886,541 + -0.5 3,886,541 + -0.4 3,886,541 + +0.2 3,886,541 + +1.3 3,886,541 + +0.6 3,886,541 + -0.8 3,886,541 + +0.2 3,886,541 + +0.2 3,886,541 + +0.7 3,886,541 + -0.5 3,886,541 + -0.0 3,886,541 + -0.7 3,886,541 + -0.3 3,886,541 + -0.8 3,886,541 + +0.4 3,886,541 + -0.1 3,886,541 + -0.5 3,886,541 + -0.6 3,886,541 + +0.1 3,886,541 + -0.1 3,886,547 + +0.4 3,886,547 + +0.3 3,886,547 + -0.4 3,886,549 + -0.3 3,886,552 + -1.1 3,886,572 + -0.8 3,886,573 + -0.0 3,886,599 + -1.5 3,886,662 + -0.3 3,886,670 + +0.7 3,886,713 + +0.7 3,886,772 + +0.6 3,886,780 - -3.2 3,886,780 - -3.0 3,886,780 - +1.6 3,886,780 - -0.3 3,886,781 - -0.1 3,886,781 - +0.8 3,886,788 + -0.7 3,886,804 - -0.7 3,886,810 - -0.2 3,886,810 - -0.4 3,886,813 - -0.9 3,886,832 - -0.4 3,886,837 - +0.0 3,886,837 - -0.3 3,886,846 + +1.3 3,886,853 - +1.6 3,886,853 - -0.0 3,886,868 - -0.0 3,886,868 - +0.7 3,886,874 - -0.2 3,886,874 - +0.3 3,886,875 + -1.0 3,886,875 + -0.2 3,886,875 + -0.4 3,886,875 + +0.2 3,886,875 - -0.8 3,886,878 - +0.2 3,886,878 - -0.3 3,886,914 - tnaA b3708 0.11 -1.8 3,886,924 - tnaA b3708 0.12 -0.6 3,886,924 - tnaA b3708 0.12 +0.0 3,886,924 - tnaA b3708 0.12 -0.1 3,886,931 + tnaA b3708 0.13 -0.6 3,886,935 - tnaA b3708 0.13 +0.9 3,886,935 - tnaA b3708 0.13 -1.2 3,886,938 - tnaA b3708 0.13 -0.4 3,886,938 - tnaA b3708 0.13 -0.6 3,886,938 - tnaA b3708 0.13 -0.5 3,886,939 - tnaA b3708 0.13 -0.2 3,886,939 - tnaA b3708 0.13 -0.3 3,886,943 + tnaA b3708 0.13 -1.7 3,886,943 - tnaA b3708 0.13 -0.2 3,886,946 - tnaA b3708 0.14 +0.2 3,886,946 - +1.6 3,886,952 - tnaA b3708 0.14 -0.0 3,886,952 - tnaA b3708 0.14 +0.4 3,886,969 + tnaA b3708 0.15 -0.1 3,886,972 - tnaA b3708 0.15 -0.1 3,886,977 - tnaA b3708 0.16 -0.2 3,886,984 + tnaA b3708 0.16 +0.4 3,886,988 + tnaA b3708 0.17 +0.2 3,886,990 - tnaA b3708 0.17 -0.9 3,886,990 - tnaA b3708 0.17 -0.5 3,886,994 - tnaA b3708 0.17 -0.4 3,886,994 - tnaA b3708 0.17 -0.0 3,886,999 + tnaA b3708 0.17 -0.8 3,887,032 + tnaA b3708 0.20 +0.4 3,887,039 + tnaA b3708 0.20 +0.4 3,887,039 - tnaA b3708 0.20 -2.2 3,887,039 - tnaA b3708 0.20 +0.4 3,887,039 - tnaA b3708 0.20 -0.8 3,887,039 - tnaA b3708 0.20 -0.5 3,887,040 - tnaA b3708 0.20 +0.2 3,887,040 - tnaA b3708 0.20 -1.6 3,887,045 + tnaA b3708 0.21 +0.6 3,887,066 - tnaA b3708 0.22 -0.3 3,887,076 - tnaA b3708 0.23 +0.6 3,887,083 - tnaA b3708 0.23 -0.0 3,887,083 - tnaA b3708 0.23 +2.2 3,887,116 + tnaA b3708 0.26 +0.5 3,887,116 + tnaA b3708 0.26 +0.1 3,887,175 - tnaA b3708 0.30 +0.6 3,887,190 + tnaA b3708 0.31 +0.1 3,887,190 + tnaA b3708 0.31 -0.1 3,887,192 + tnaA b3708 0.31 +0.1 3,887,192 + tnaA b3708 0.31 -0.0 3,887,192 + tnaA b3708 0.31 -0.5 3,887,192 - tnaA b3708 0.31 -0.6 3,887,197 + tnaA b3708 0.31 +0.4 3,887,197 + tnaA b3708 0.31 -0.2 3,887,198 - tnaA b3708 0.31 -0.9 3,887,200 + tnaA b3708 0.32 +0.9
Or see this region's nucleotide sequence