Strain Fitness in Escherichia coli BW25113 around b2567

Experiment: Trimethoprim 0.00125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrecO and era are separated by 11 nucleotidesera and rnc overlap by 4 nucleotidesrnc and lepB are separated by 271 nucleotides b2565: recO - DNA repair protein RecO (NCBI), at 2,699,763 to 2,700,491 recO b2566: era - GTP-binding protein Era (NCBI), at 2,700,503 to 2,701,408 era b2567: rnc - ribonuclease III (NCBI), at 2,701,405 to 2,702,085 rnc b2568: lepB - leader peptidase (signal peptidase I) (NCBI), at 2,702,357 to 2,703,331 lepB Position (kb) 2701 2702 2703Strain fitness (log2 ratio) -2 -1 0 1at 2700.467 kb on - strandat 2701.692 kb on - strand, within rncat 2702.009 kb on - strand, within rncat 2702.100 kb on - strandat 2702.134 kb on - strandat 2702.144 kb on - strandat 2702.144 kb on - strandat 2702.147 kb on - strandat 2702.147 kb on - strandat 2702.164 kb on - strandat 2702.164 kb on - strandat 2702.191 kb on - strandat 2702.191 kb on - strandat 2702.191 kb on - strandat 2702.229 kb on - strandat 2702.303 kb on + strandat 2702.368 kb on + strandat 2702.368 kb on + strandat 2702.439 kb on - strandat 2702.448 kb on - strandat 2702.448 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.00125 mM
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2,700,467 - +0.0
2,701,692 - rnc b2567 0.42 +1.1
2,702,009 - rnc b2567 0.89 +1.1
2,702,100 - +0.3
2,702,134 - +1.5
2,702,144 - +0.2
2,702,144 - -1.0
2,702,147 - +0.1
2,702,147 - +0.1
2,702,164 - -0.2
2,702,164 - +1.4
2,702,191 - +0.8
2,702,191 - +0.6
2,702,191 - -0.4
2,702,229 - +0.9
2,702,303 + +0.1
2,702,368 + -1.9
2,702,368 + -0.4
2,702,439 - +0.1
2,702,448 - -1.1
2,702,448 - +0.7

Or see this region's nucleotide sequence