Strain Fitness in Escherichia coli BW25113 around b1766

Experiment: Trimethoprim 0.00125 mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntselD and ydjA are separated by 116 nucleotidesydjA and sppA are separated by 160 nucleotidessppA and ansA are separated by 166 nucleotides b1764: selD - selenophosphate synthetase (NCBI), at 1,844,989 to 1,846,032 selD b1765: ydjA - predicted oxidoreductase (NCBI), at 1,846,149 to 1,846,700 ydjA b1766: sppA - protease IV (signal peptide peptidase) (NCBI), at 1,846,861 to 1,848,717 sppA b1767: ansA - cytoplasmic asparaginase I (NCBI), at 1,848,884 to 1,849,900 ansA Position (kb) 1846 1847 1848 1849Strain fitness (log2 ratio) -1 0 1 2at 1845.881 kb on - strand, within selDat 1845.883 kb on - strand, within selDat 1846.260 kb on + strand, within ydjAat 1846.671 kb on - strandat 1846.671 kb on - strandat 1846.826 kb on - strandat 1846.828 kb on + strandat 1846.843 kb on - strandat 1846.849 kb on - strandat 1846.849 kb on - strandat 1846.860 kb on + strandat 1846.860 kb on + strandat 1846.910 kb on - strandat 1847.043 kb on - strandat 1847.186 kb on - strand, within sppAat 1847.251 kb on + strand, within sppAat 1847.251 kb on + strand, within sppAat 1847.481 kb on + strand, within sppAat 1847.481 kb on + strandat 1847.547 kb on - strand, within sppAat 1847.614 kb on - strand, within sppAat 1847.630 kb on - strand, within sppAat 1847.630 kb on - strand, within sppAat 1847.845 kb on + strand, within sppAat 1847.845 kb on + strand, within sppAat 1847.975 kb on + strand, within sppAat 1848.206 kb on + strand, within sppAat 1848.219 kb on - strand, within sppAat 1848.280 kb on + strand, within sppAat 1848.374 kb on + strand, within sppAat 1848.374 kb on + strand, within sppAat 1848.390 kb on + strand, within sppAat 1848.390 kb on + strand, within sppAat 1848.398 kb on - strand, within sppAat 1848.726 kb on - strandat 1848.833 kb on - strandat 1848.833 kb on - strandat 1848.842 kb on + strandat 1848.842 kb on + strandat 1848.850 kb on - strandat 1848.979 kb on - strandat 1848.979 kb on - strandat 1849.035 kb on - strand, within ansAat 1849.123 kb on + strand, within ansAat 1849.152 kb on + strand, within ansAat 1849.152 kb on + strand, within ansAat 1849.160 kb on - strand, within ansAat 1849.160 kb on - strand, within ansAat 1849.173 kb on - strand, within ansAat 1849.184 kb on - strand, within ansAat 1849.260 kb on - strand, within ansAat 1849.474 kb on - strand, within ansAat 1849.474 kb on - strand, within ansAat 1849.603 kb on + strand, within ansA

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.00125 mM
remove
1,845,881 - selD b1764 0.85 -0.5
1,845,883 - selD b1764 0.86 -0.0
1,846,260 + ydjA b1765 0.20 +0.2
1,846,671 - -1.5
1,846,671 - +0.5
1,846,826 - +1.6
1,846,828 + +0.1
1,846,843 - +0.9
1,846,849 - +0.7
1,846,849 - -0.8
1,846,860 + -0.9
1,846,860 + -0.9
1,846,910 - +0.7
1,847,043 - +1.2
1,847,186 - sppA b1766 0.18 +0.4
1,847,251 + sppA b1766 0.21 +0.6
1,847,251 + sppA b1766 0.21 +0.8
1,847,481 + sppA b1766 0.33 +2.0
1,847,481 + +1.1
1,847,547 - sppA b1766 0.37 +0.5
1,847,614 - sppA b1766 0.41 +0.5
1,847,630 - sppA b1766 0.41 -0.0
1,847,630 - sppA b1766 0.41 +2.2
1,847,845 + sppA b1766 0.53 +0.8
1,847,845 + sppA b1766 0.53 +1.4
1,847,975 + sppA b1766 0.60 +1.5
1,848,206 + sppA b1766 0.72 +0.9
1,848,219 - sppA b1766 0.73 +0.9
1,848,280 + sppA b1766 0.76 +1.3
1,848,374 + sppA b1766 0.81 +1.2
1,848,374 + sppA b1766 0.81 +0.9
1,848,390 + sppA b1766 0.82 +0.5
1,848,390 + sppA b1766 0.82 +0.8
1,848,398 - sppA b1766 0.83 +1.7
1,848,726 - -0.1
1,848,833 - -0.0
1,848,833 - -0.6
1,848,842 + -0.7
1,848,842 + -0.7
1,848,850 - +0.1
1,848,979 - -0.6
1,848,979 - -0.5
1,849,035 - ansA b1767 0.15 -0.6
1,849,123 + ansA b1767 0.24 +1.3
1,849,152 + ansA b1767 0.26 -0.2
1,849,152 + ansA b1767 0.26 +0.9
1,849,160 - ansA b1767 0.27 -0.9
1,849,160 - ansA b1767 0.27 -0.9
1,849,173 - ansA b1767 0.28 -0.9
1,849,184 - ansA b1767 0.29 +0.4
1,849,260 - ansA b1767 0.37 -0.1
1,849,474 - ansA b1767 0.58 +0.5
1,849,474 - ansA b1767 0.58 -0.7
1,849,603 + ansA b1767 0.71 +0.4

Or see this region's nucleotide sequence