Strain Fitness in Escherichia coli BW25113 around b1334

Experiment: Trimethoprim 0.00125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntuspE and fnr are separated by 151 nucleotidesfnr and ogt are separated by 194 nucleotidesogt and ydaH are separated by 10 nucleotides b1333: uspE - stress-induced protein (NCBI), at 1,395,696 to 1,396,646 uspE b1334: fnr - DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI), at 1,396,798 to 1,397,550 fnr b1335: ogt - O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI), at 1,397,745 to 1,398,260 ogt b1336: ydaH - putative pump protein (transport) (VIMSS), at 1,398,271 to 1,399,797 ydaH Position (kb) 1396 1397 1398Strain fitness (log2 ratio) -2 -1 0 1 2at 1396.148 kb on + strand, within uspEat 1396.214 kb on - strand, within uspEat 1396.295 kb on - strand, within uspEat 1396.341 kb on + strand, within uspEat 1396.604 kb on + strandat 1396.609 kb on + strandat 1396.609 kb on + strandat 1396.728 kb on - strandat 1396.728 kb on - strandat 1397.086 kb on + strand, within fnrat 1397.086 kb on + strand, within fnrat 1397.125 kb on - strand, within fnrat 1397.125 kb on - strand, within fnrat 1397.287 kb on - strand, within fnrat 1397.605 kb on + strandat 1397.605 kb on + strandat 1397.857 kb on - strand, within ogtat 1397.857 kb on - strand, within ogtat 1397.903 kb on + strand, within ogtat 1397.903 kb on + strand, within ogtat 1398.130 kb on + strand, within ogtat 1398.130 kb on + strand, within ogtat 1398.138 kb on + strand, within ogtat 1398.138 kb on + strand, within ogt

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.00125 mM
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1,396,148 + uspE b1333 0.48 -2.3
1,396,214 - uspE b1333 0.54 -0.9
1,396,295 - uspE b1333 0.63 -0.7
1,396,341 + uspE b1333 0.68 -0.3
1,396,604 + -0.1
1,396,609 + -0.4
1,396,609 + -0.7
1,396,728 - -0.2
1,396,728 - +0.4
1,397,086 + fnr b1334 0.38 +2.2
1,397,086 + fnr b1334 0.38 +1.2
1,397,125 - fnr b1334 0.43 +0.5
1,397,125 - fnr b1334 0.43 +0.7
1,397,287 - fnr b1334 0.65 -1.1
1,397,605 + +0.5
1,397,605 + +0.5
1,397,857 - ogt b1335 0.22 +0.8
1,397,857 - ogt b1335 0.22 +0.4
1,397,903 + ogt b1335 0.31 +0.4
1,397,903 + ogt b1335 0.31 -0.7
1,398,130 + ogt b1335 0.75 -1.2
1,398,130 + ogt b1335 0.75 -1.7
1,398,138 + ogt b1335 0.76 -0.9
1,398,138 + ogt b1335 0.76 +0.1

Or see this region's nucleotide sequence