Strain Fitness in Escherichia coli BW25113 around b1106

Experiment: Trimethoprim 0.00125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycfL and ycfM are separated by 13 nucleotidesycfM and ycfN overlap by 20 nucleotidesycfN and nagZ are separated by 10 nucleotides b1104: ycfL - hypothetical protein (NCBI), at 1,161,470 to 1,161,847 ycfL b1105: ycfM - predicted outer membrane lipoprotein (NCBI), at 1,161,861 to 1,162,502 ycfM b1106: ycfN - thiamin kinase (NCBI), at 1,162,483 to 1,163,307 ycfN b1107: nagZ - beta-hexosaminidase (NCBI), at 1,163,318 to 1,164,343 nagZ Position (kb) 1162 1163 1164Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1161.508 kb on - strand, within ycfLat 1161.648 kb on - strand, within ycfLat 1161.659 kb on + strand, within ycfLat 1161.996 kb on + strand, within ycfMat 1162.014 kb on + strand, within ycfMat 1162.014 kb on + strand, within ycfMat 1162.190 kb on - strand, within ycfMat 1162.347 kb on - strand, within ycfMat 1162.456 kb on - strandat 1162.640 kb on + strand, within ycfNat 1162.873 kb on - strand, within ycfNat 1162.875 kb on + strand, within ycfNat 1162.992 kb on - strand, within ycfNat 1163.024 kb on + strand, within ycfNat 1163.057 kb on - strand, within ycfNat 1163.213 kb on - strand, within ycfNat 1163.219 kb on + strand, within ycfNat 1163.220 kb on + strand, within ycfNat 1163.313 kb on + strandat 1163.324 kb on - strandat 1163.576 kb on + strand, within nagZat 1163.576 kb on + strand, within nagZat 1163.630 kb on - strand, within nagZat 1163.687 kb on + strand, within nagZat 1163.695 kb on - strand, within nagZat 1163.695 kb on - strand, within nagZat 1163.772 kb on + strand, within nagZat 1163.819 kb on - strand, within nagZat 1163.831 kb on - strand, within nagZat 1163.831 kb on - strand, within nagZat 1163.855 kb on - strand, within nagZat 1163.949 kb on - strand, within nagZat 1163.949 kb on - strand, within nagZ

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.00125 mM
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1,161,508 - ycfL b1104 0.10 -0.1
1,161,648 - ycfL b1104 0.47 -0.5
1,161,659 + ycfL b1104 0.50 -1.8
1,161,996 + ycfM b1105 0.21 -3.5
1,162,014 + ycfM b1105 0.24 -1.5
1,162,014 + ycfM b1105 0.24 -3.2
1,162,190 - ycfM b1105 0.51 -3.5
1,162,347 - ycfM b1105 0.76 -3.0
1,162,456 - -3.6
1,162,640 + ycfN b1106 0.19 -0.1
1,162,873 - ycfN b1106 0.47 +0.4
1,162,875 + ycfN b1106 0.48 +2.1
1,162,992 - ycfN b1106 0.62 +0.4
1,163,024 + ycfN b1106 0.66 +0.2
1,163,057 - ycfN b1106 0.70 +0.2
1,163,213 - ycfN b1106 0.88 -0.1
1,163,219 + ycfN b1106 0.89 +1.3
1,163,220 + ycfN b1106 0.89 +0.9
1,163,313 + +0.4
1,163,324 - -0.5
1,163,576 + nagZ b1107 0.25 -0.4
1,163,576 + nagZ b1107 0.25 -0.2
1,163,630 - nagZ b1107 0.30 -0.9
1,163,687 + nagZ b1107 0.36 +0.7
1,163,695 - nagZ b1107 0.37 +1.2
1,163,695 - nagZ b1107 0.37 -1.6
1,163,772 + nagZ b1107 0.44 +0.6
1,163,819 - nagZ b1107 0.49 +1.3
1,163,831 - nagZ b1107 0.50 +0.1
1,163,831 - nagZ b1107 0.50 +0.9
1,163,855 - nagZ b1107 0.52 +0.1
1,163,949 - nagZ b1107 0.62 +0.9
1,163,949 - nagZ b1107 0.62 -0.4

Or see this region's nucleotide sequence