Experiment: Trimethoprim 0.00125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purK and purE overlap by 4 nucleotides purE and lpxH are separated by 117 nucleotides lpxH and ppiB are separated by 2 nucleotides
b0522: purK - phosphoribosylaminoimidazole carboxylase (NCBI), at 550,750 to 551,817
purK
b0523: purE - phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI), at 551,814 to 552,323
purE
b0524: lpxH - UDP-2,3-diacylglucosamine hydrolase (NCBI), at 552,441 to 553,163
lpxH
b0525: ppiB - peptidyl-prolyl cis-trans isomerase B (rotamase B) (NCBI), at 553,166 to 553,660
ppiB
Position (kb) 551
552
553 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 550.959 kb on + strand, within purK at 550.977 kb on - strand, within purK at 551.057 kb on + strand, within purK at 551.062 kb on + strand, within purK at 551.062 kb on + strand, within purK at 551.091 kb on - strand, within purK at 551.095 kb on + strand, within purK at 551.095 kb on + strand, within purK at 551.112 kb on + strand, within purK at 551.112 kb on + strand, within purK at 551.144 kb on + strand, within purK at 551.162 kb on - strand, within purK at 551.162 kb on - strand, within purK at 551.217 kb on + strand, within purK at 551.227 kb on - strand, within purK at 551.236 kb on + strand, within purK at 551.236 kb on + strand, within purK at 551.255 kb on + strand, within purK at 551.271 kb on + strand, within purK at 551.356 kb on + strand, within purK at 551.375 kb on + strand, within purK at 551.391 kb on - strand, within purK at 551.458 kb on - strand, within purK at 551.532 kb on + strand, within purK at 551.532 kb on + strand, within purK at 551.543 kb on + strand, within purK at 551.591 kb on - strand, within purK at 551.591 kb on - strand, within purK at 551.649 kb on - strand, within purK at 551.772 kb on + strand at 551.878 kb on + strand, within purE at 552.015 kb on - strand, within purE at 552.027 kb on + strand, within purE at 552.035 kb on - strand, within purE at 552.052 kb on - strand, within purE at 552.252 kb on + strand, within purE at 552.350 kb on + strand at 552.350 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trimethoprim 0.00125 mM remove 550,959 + purK b0522 0.20 -0.8 550,977 - purK b0522 0.21 +0.4 551,057 + purK b0522 0.29 -0.8 551,062 + purK b0522 0.29 +1.2 551,062 + purK b0522 0.29 -0.4 551,091 - purK b0522 0.32 -0.4 551,095 + purK b0522 0.32 -1.0 551,095 + purK b0522 0.32 -2.6 551,112 + purK b0522 0.34 +0.5 551,112 + purK b0522 0.34 -2.9 551,144 + purK b0522 0.37 +1.0 551,162 - purK b0522 0.39 +0.2 551,162 - purK b0522 0.39 -0.0 551,217 + purK b0522 0.44 -0.1 551,227 - purK b0522 0.45 -2.0 551,236 + purK b0522 0.46 +0.4 551,236 + purK b0522 0.46 +0.4 551,255 + purK b0522 0.47 +0.9 551,271 + purK b0522 0.49 -0.1 551,356 + purK b0522 0.57 -0.1 551,375 + purK b0522 0.59 +1.1 551,391 - purK b0522 0.60 -0.1 551,458 - purK b0522 0.66 +0.0 551,532 + purK b0522 0.73 +1.1 551,532 + purK b0522 0.73 -0.1 551,543 + purK b0522 0.74 -0.1 551,591 - purK b0522 0.79 -0.4 551,591 - purK b0522 0.79 +0.1 551,649 - purK b0522 0.84 -0.3 551,772 + -0.6 551,878 + purE b0523 0.13 +1.8 552,015 - purE b0523 0.39 +1.7 552,027 + purE b0523 0.42 +1.4 552,035 - purE b0523 0.43 +2.1 552,052 - purE b0523 0.47 -0.5 552,252 + purE b0523 0.86 +1.2 552,350 + +0.6 552,350 + +0.2
Or see this region's nucleotide sequence