Experiment: Trimethoprim 0.00125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybbW and allB are separated by 59 nucleotides allB and ybbY are separated by 56 nucleotides
b0511: ybbW - putative transport protein (VIMSS), at 536,857 to 538,311
ybbW
b0512: allB - allantoinase (NCBI), at 538,371 to 539,732
allB
b0513: ybbY - putative transport (VIMSS), at 539,789 to 541,090
ybbY
Position (kb) 538
539
540 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 537.421 kb on - strand, within ybbW at 537.423 kb on - strand, within ybbW at 537.423 kb on - strand, within ybbW at 537.437 kb on - strand, within ybbW at 537.437 kb on - strand, within ybbW at 537.479 kb on - strand, within ybbW at 537.479 kb on - strand, within ybbW at 537.479 kb on - strand, within ybbW at 537.479 kb on - strand, within ybbW at 537.479 kb on - strand, within ybbW at 537.517 kb on - strand, within ybbW at 537.551 kb on + strand, within ybbW at 537.551 kb on + strand, within ybbW at 537.552 kb on + strand, within ybbW at 537.565 kb on + strand, within ybbW at 537.583 kb on - strand, within ybbW at 537.583 kb on - strand, within ybbW at 537.644 kb on + strand, within ybbW at 537.644 kb on + strand, within ybbW at 537.659 kb on + strand, within ybbW at 537.669 kb on - strand, within ybbW at 537.669 kb on - strand, within ybbW at 537.676 kb on + strand, within ybbW at 537.678 kb on + strand, within ybbW at 537.698 kb on + strand, within ybbW at 537.702 kb on - strand, within ybbW at 537.716 kb on + strand, within ybbW at 537.718 kb on + strand, within ybbW at 537.718 kb on + strand, within ybbW at 537.719 kb on - strand, within ybbW at 537.724 kb on - strand, within ybbW at 537.726 kb on - strand, within ybbW at 537.726 kb on - strand, within ybbW at 537.730 kb on + strand, within ybbW at 537.750 kb on + strand, within ybbW at 537.750 kb on + strand, within ybbW at 537.757 kb on + strand, within ybbW at 537.757 kb on + strand, within ybbW at 537.762 kb on + strand, within ybbW at 537.762 kb on + strand, within ybbW at 537.762 kb on + strand, within ybbW at 537.762 kb on + strand, within ybbW at 537.767 kb on + strand, within ybbW at 537.773 kb on + strand, within ybbW at 537.773 kb on + strand, within ybbW at 537.773 kb on + strand, within ybbW at 537.773 kb on + strand, within ybbW at 537.774 kb on + strand, within ybbW at 537.775 kb on - strand, within ybbW at 537.791 kb on + strand, within ybbW at 537.791 kb on + strand, within ybbW at 537.810 kb on + strand, within ybbW at 537.820 kb on + strand, within ybbW at 537.822 kb on + strand, within ybbW at 537.827 kb on - strand, within ybbW at 537.827 kb on - strand, within ybbW at 537.857 kb on + strand, within ybbW at 537.857 kb on + strand, within ybbW at 537.907 kb on + strand, within ybbW at 537.922 kb on + strand, within ybbW at 537.929 kb on + strand, within ybbW at 538.060 kb on + strand, within ybbW at 538.134 kb on + strand, within ybbW at 538.190 kb on + strand at 538.190 kb on + strand at 538.246 kb on + strand at 538.253 kb on + strand at 538.266 kb on + strand at 538.266 kb on + strand at 538.269 kb on + strand at 538.269 kb on + strand at 538.291 kb on + strand at 538.313 kb on - strand at 538.370 kb on - strand at 538.437 kb on - strand at 538.484 kb on - strand at 538.484 kb on - strand at 538.494 kb on - strand at 538.494 kb on - strand at 538.507 kb on + strand at 538.515 kb on - strand, within allB at 538.515 kb on - strand, within allB at 538.515 kb on - strand, within allB at 538.567 kb on + strand, within allB at 538.593 kb on - strand, within allB at 538.660 kb on - strand, within allB at 538.736 kb on + strand, within allB at 538.745 kb on + strand, within allB at 538.752 kb on - strand, within allB at 538.752 kb on - strand, within allB at 538.815 kb on + strand, within allB at 538.828 kb on - strand, within allB at 538.920 kb on + strand, within allB at 538.925 kb on - strand, within allB at 539.002 kb on + strand, within allB at 539.002 kb on + strand, within allB at 539.008 kb on - strand, within allB at 539.112 kb on + strand, within allB at 539.145 kb on + strand, within allB at 539.145 kb on + strand, within allB at 539.145 kb on + strand, within allB at 539.180 kb on + strand, within allB at 539.328 kb on - strand, within allB at 539.351 kb on + strand, within allB at 539.351 kb on + strand, within allB at 539.351 kb on + strand, within allB at 539.364 kb on - strand, within allB at 539.397 kb on + strand, within allB at 539.622 kb on - strand at 539.686 kb on + strand at 539.731 kb on + strand at 539.739 kb on - strand at 539.951 kb on + strand, within ybbY at 539.964 kb on - strand, within ybbY at 539.964 kb on - strand, within ybbY at 539.966 kb on + strand, within ybbY at 539.969 kb on - strand, within ybbY at 539.969 kb on - strand, within ybbY at 539.996 kb on + strand, within ybbY at 540.014 kb on - strand, within ybbY at 540.014 kb on - strand, within ybbY at 540.105 kb on + strand, within ybbY at 540.149 kb on + strand, within ybbY at 540.315 kb on + strand, within ybbY at 540.325 kb on + strand, within ybbY at 540.325 kb on + strand, within ybbY at 540.404 kb on - strand, within ybbY at 540.404 kb on - strand, within ybbY at 540.446 kb on + strand, within ybbY at 540.446 kb on + strand, within ybbY at 540.455 kb on - strand, within ybbY at 540.455 kb on - strand, within ybbY at 540.484 kb on - strand, within ybbY at 540.502 kb on + strand, within ybbY at 540.526 kb on + strand, within ybbY at 540.531 kb on - strand, within ybbY at 540.554 kb on + strand, within ybbY at 540.554 kb on + strand, within ybbY at 540.593 kb on + strand, within ybbY at 540.593 kb on + strand, within ybbY at 540.607 kb on + strand, within ybbY at 540.607 kb on + strand, within ybbY at 540.607 kb on + strand, within ybbY at 540.607 kb on + strand, within ybbY at 540.612 kb on + strand, within ybbY at 540.613 kb on + strand, within ybbY at 540.622 kb on + strand, within ybbY at 540.622 kb on + strand, within ybbY
Per-strain Table
Position Strand Gene LocusTag Fraction Trimethoprim 0.00125 mM remove 537,421 - ybbW b0511 0.39 +1.0 537,423 - ybbW b0511 0.39 +1.7 537,423 - ybbW b0511 0.39 +0.7 537,437 - ybbW b0511 0.40 +1.7 537,437 - ybbW b0511 0.40 +0.1 537,479 - ybbW b0511 0.43 +0.1 537,479 - ybbW b0511 0.43 +1.4 537,479 - ybbW b0511 0.43 +0.1 537,479 - ybbW b0511 0.43 +1.0 537,479 - ybbW b0511 0.43 +0.1 537,517 - ybbW b0511 0.45 +1.1 537,551 + ybbW b0511 0.48 +1.3 537,551 + ybbW b0511 0.48 +0.1 537,552 + ybbW b0511 0.48 +0.5 537,565 + ybbW b0511 0.49 +0.4 537,583 - ybbW b0511 0.50 +0.1 537,583 - ybbW b0511 0.50 +3.3 537,644 + ybbW b0511 0.54 -0.6 537,644 + ybbW b0511 0.54 +0.1 537,659 + ybbW b0511 0.55 -0.0 537,669 - ybbW b0511 0.56 -0.5 537,669 - ybbW b0511 0.56 +2.7 537,676 + ybbW b0511 0.56 +0.1 537,678 + ybbW b0511 0.56 -0.6 537,698 + ybbW b0511 0.58 +0.1 537,702 - ybbW b0511 0.58 +1.4 537,716 + ybbW b0511 0.59 -0.5 537,718 + ybbW b0511 0.59 -0.2 537,718 + ybbW b0511 0.59 -1.5 537,719 - ybbW b0511 0.59 -1.0 537,724 - ybbW b0511 0.60 +1.3 537,726 - ybbW b0511 0.60 -1.7 537,726 - ybbW b0511 0.60 +0.3 537,730 + ybbW b0511 0.60 -0.9 537,750 + ybbW b0511 0.61 -0.5 537,750 + ybbW b0511 0.61 -1.8 537,757 + ybbW b0511 0.62 -0.6 537,757 + ybbW b0511 0.62 +1.5 537,762 + ybbW b0511 0.62 +1.3 537,762 + ybbW b0511 0.62 +0.5 537,762 + ybbW b0511 0.62 -1.1 537,762 + ybbW b0511 0.62 +1.0 537,767 + ybbW b0511 0.63 -2.5 537,773 + ybbW b0511 0.63 -0.0 537,773 + ybbW b0511 0.63 -0.9 537,773 + ybbW b0511 0.63 -0.0 537,773 + ybbW b0511 0.63 +0.4 537,774 + ybbW b0511 0.63 +0.2 537,775 - ybbW b0511 0.63 +0.7 537,791 + ybbW b0511 0.64 -0.2 537,791 + ybbW b0511 0.64 -0.5 537,810 + ybbW b0511 0.65 -0.6 537,820 + ybbW b0511 0.66 +1.0 537,822 + ybbW b0511 0.66 -0.5 537,827 - ybbW b0511 0.67 -0.2 537,827 - ybbW b0511 0.67 +1.7 537,857 + ybbW b0511 0.69 +3.0 537,857 + ybbW b0511 0.69 +0.7 537,907 + ybbW b0511 0.72 +1.7 537,922 + ybbW b0511 0.73 +0.4 537,929 + ybbW b0511 0.74 -0.9 538,060 + ybbW b0511 0.83 +0.7 538,134 + ybbW b0511 0.88 +1.7 538,190 + +0.7 538,190 + +1.7 538,246 + +0.2 538,253 + -2.3 538,266 + +1.7 538,266 + +1.4 538,269 + +1.7 538,269 + -0.8 538,291 + -0.4 538,313 - +1.7 538,370 - -0.9 538,437 - +0.2 538,484 - +1.7 538,484 - -0.3 538,494 - +0.1 538,494 - -0.6 538,507 + -0.1 538,515 - allB b0512 0.11 -0.3 538,515 - allB b0512 0.11 +0.7 538,515 - allB b0512 0.11 +1.7 538,567 + allB b0512 0.14 -0.6 538,593 - allB b0512 0.16 +0.2 538,660 - allB b0512 0.21 -0.6 538,736 + allB b0512 0.27 +0.7 538,745 + allB b0512 0.27 -1.2 538,752 - allB b0512 0.28 -0.6 538,752 - allB b0512 0.28 +0.9 538,815 + allB b0512 0.33 -0.9 538,828 - allB b0512 0.34 +0.4 538,920 + allB b0512 0.40 -0.0 538,925 - allB b0512 0.41 +2.4 539,002 + allB b0512 0.46 -0.4 539,002 + allB b0512 0.46 +0.5 539,008 - allB b0512 0.47 -0.7 539,112 + allB b0512 0.54 +0.3 539,145 + allB b0512 0.57 +0.3 539,145 + allB b0512 0.57 +0.2 539,145 + allB b0512 0.57 -0.9 539,180 + allB b0512 0.59 -0.3 539,328 - allB b0512 0.70 +0.2 539,351 + allB b0512 0.72 -0.6 539,351 + allB b0512 0.72 +0.3 539,351 + allB b0512 0.72 -0.1 539,364 - allB b0512 0.73 -0.2 539,397 + allB b0512 0.75 -0.5 539,622 - -0.1 539,686 + -0.7 539,731 + +0.2 539,739 - +0.3 539,951 + ybbY b0513 0.12 -1.6 539,964 - ybbY b0513 0.13 +1.3 539,964 - ybbY b0513 0.13 -1.2 539,966 + ybbY b0513 0.14 +0.0 539,969 - ybbY b0513 0.14 +0.4 539,969 - ybbY b0513 0.14 +1.2 539,996 + ybbY b0513 0.16 -0.6 540,014 - ybbY b0513 0.17 +0.4 540,014 - ybbY b0513 0.17 +0.1 540,105 + ybbY b0513 0.24 -0.6 540,149 + ybbY b0513 0.28 -0.1 540,315 + ybbY b0513 0.40 +0.3 540,325 + ybbY b0513 0.41 -0.8 540,325 + ybbY b0513 0.41 +0.2 540,404 - ybbY b0513 0.47 -1.2 540,404 - ybbY b0513 0.47 +0.3 540,446 + ybbY b0513 0.50 -0.5 540,446 + ybbY b0513 0.50 -2.3 540,455 - ybbY b0513 0.51 -0.2 540,455 - ybbY b0513 0.51 -0.1 540,484 - ybbY b0513 0.53 +1.1 540,502 + ybbY b0513 0.55 +0.2 540,526 + ybbY b0513 0.57 +0.0 540,531 - ybbY b0513 0.57 +0.7 540,554 + ybbY b0513 0.59 +1.3 540,554 + ybbY b0513 0.59 +1.0 540,593 + ybbY b0513 0.62 -0.0 540,593 + ybbY b0513 0.62 -1.4 540,607 + ybbY b0513 0.63 -0.2 540,607 + ybbY b0513 0.63 -0.4 540,607 + ybbY b0513 0.63 -0.5 540,607 + ybbY b0513 0.63 -1.4 540,612 + ybbY b0513 0.63 -0.0 540,613 + ybbY b0513 0.63 +0.6 540,622 + ybbY b0513 0.64 -1.5 540,622 + ybbY b0513 0.64 -0.1
Or see this region's nucleotide sequence