Experiment: Trimethoprim 0.00125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mdlA and mdlB overlap by 8 nucleotides mdlB and glnK are separated by 180 nucleotides glnK and amtB are separated by 29 nucleotides
b0448: mdlA - fused predicted multidrug transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 468,095 to 469,867
mdlA
b0449: mdlB - fused predicted multidrug transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 469,860 to 471,641
mdlB
b0450: glnK - nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB (NCBI), at 471,822 to 472,160
glnK
b0451: amtB - ammonium transporter (NCBI), at 472,190 to 473,476
amtB
Position (kb) 469
470
471
472 Strain fitness (log2 ratio) -1
0
1
2 at 468.893 kb on + strand, within mdlA at 468.901 kb on + strand, within mdlA at 468.901 kb on - strand, within mdlA at 468.914 kb on - strand, within mdlA at 468.914 kb on - strand, within mdlA at 468.914 kb on - strand, within mdlA at 468.914 kb on - strand, within mdlA at 468.923 kb on - strand, within mdlA at 468.930 kb on + strand, within mdlA at 468.942 kb on + strand, within mdlA at 468.950 kb on - strand, within mdlA at 469.008 kb on + strand, within mdlA at 469.011 kb on - strand, within mdlA at 469.117 kb on + strand, within mdlA at 469.123 kb on - strand, within mdlA at 469.123 kb on - strand, within mdlA at 469.284 kb on + strand, within mdlA at 469.288 kb on - strand, within mdlA at 469.385 kb on + strand, within mdlA at 469.385 kb on + strand, within mdlA at 469.475 kb on + strand, within mdlA at 469.509 kb on - strand, within mdlA at 469.509 kb on - strand, within mdlA at 469.526 kb on + strand, within mdlA at 469.526 kb on + strand, within mdlA at 469.643 kb on - strand, within mdlA at 469.652 kb on - strand, within mdlA at 469.677 kb on + strand, within mdlA at 469.677 kb on + strand, within mdlA at 469.686 kb on + strand, within mdlA at 469.772 kb on - strand at 469.777 kb on - strand at 469.803 kb on - strand at 469.830 kb on - strand at 469.830 kb on - strand at 469.887 kb on + strand at 469.887 kb on + strand at 469.921 kb on + strand at 469.921 kb on + strand at 469.955 kb on - strand at 469.965 kb on - strand at 469.965 kb on - strand at 469.965 kb on - strand at 469.965 kb on - strand at 470.079 kb on - strand, within mdlB at 470.079 kb on - strand, within mdlB at 470.166 kb on + strand, within mdlB at 470.189 kb on - strand, within mdlB at 470.259 kb on - strand, within mdlB at 470.391 kb on + strand, within mdlB at 470.406 kb on - strand, within mdlB at 470.450 kb on + strand, within mdlB at 470.575 kb on - strand, within mdlB at 470.575 kb on - strand, within mdlB at 470.582 kb on + strand, within mdlB at 470.667 kb on + strand, within mdlB at 470.694 kb on + strand, within mdlB at 470.780 kb on + strand, within mdlB at 470.788 kb on - strand, within mdlB at 470.788 kb on - strand, within mdlB at 470.805 kb on - strand, within mdlB at 470.838 kb on + strand, within mdlB at 470.838 kb on + strand, within mdlB at 471.033 kb on - strand, within mdlB at 471.033 kb on - strand, within mdlB at 471.076 kb on - strand, within mdlB at 471.076 kb on - strand, within mdlB at 471.119 kb on - strand, within mdlB at 471.119 kb on - strand, within mdlB at 471.135 kb on + strand, within mdlB at 471.224 kb on + strand, within mdlB at 471.410 kb on - strand, within mdlB at 471.433 kb on + strand, within mdlB at 471.443 kb on + strand, within mdlB at 471.447 kb on - strand, within mdlB at 471.510 kb on - strand at 471.522 kb on - strand at 471.549 kb on + strand at 471.551 kb on + strand at 471.551 kb on + strand at 471.679 kb on + strand at 471.692 kb on - strand at 471.699 kb on - strand at 471.866 kb on + strand, within glnK at 471.866 kb on + strand, within glnK at 471.884 kb on - strand, within glnK at 472.069 kb on - strand, within glnK at 472.140 kb on + strand at 472.180 kb on - strand at 472.254 kb on - strand at 472.257 kb on + strand at 472.345 kb on + strand, within amtB at 472.345 kb on + strand, within amtB at 472.415 kb on - strand, within amtB at 472.417 kb on + strand, within amtB at 472.430 kb on - strand, within amtB at 472.444 kb on - strand, within amtB at 472.546 kb on + strand, within amtB
Per-strain Table
Position Strand Gene LocusTag Fraction Trimethoprim 0.00125 mM remove 468,893 + mdlA b0448 0.45 +1.3 468,901 + mdlA b0448 0.45 +1.0 468,901 - mdlA b0448 0.45 +1.1 468,914 - mdlA b0448 0.46 +1.0 468,914 - mdlA b0448 0.46 -0.0 468,914 - mdlA b0448 0.46 +0.5 468,914 - mdlA b0448 0.46 +2.3 468,923 - mdlA b0448 0.47 +0.9 468,930 + mdlA b0448 0.47 +1.1 468,942 + mdlA b0448 0.48 +1.3 468,950 - mdlA b0448 0.48 +0.7 469,008 + mdlA b0448 0.51 +1.8 469,011 - mdlA b0448 0.52 +1.3 469,117 + mdlA b0448 0.58 +1.1 469,123 - mdlA b0448 0.58 +1.9 469,123 - mdlA b0448 0.58 +0.7 469,284 + mdlA b0448 0.67 +1.0 469,288 - mdlA b0448 0.67 +1.1 469,385 + mdlA b0448 0.73 +0.4 469,385 + mdlA b0448 0.73 +0.8 469,475 + mdlA b0448 0.78 +0.6 469,509 - mdlA b0448 0.80 +0.6 469,509 - mdlA b0448 0.80 +0.7 469,526 + mdlA b0448 0.81 +1.5 469,526 + mdlA b0448 0.81 +1.7 469,643 - mdlA b0448 0.87 +0.7 469,652 - mdlA b0448 0.88 +0.5 469,677 + mdlA b0448 0.89 +1.5 469,677 + mdlA b0448 0.89 +0.6 469,686 + mdlA b0448 0.90 +1.2 469,772 - +1.2 469,777 - +2.1 469,803 - -0.2 469,830 - +0.9 469,830 - +2.4 469,887 + +0.6 469,887 + +0.3 469,921 + +1.5 469,921 + +1.9 469,955 - +1.2 469,965 - +2.2 469,965 - +1.6 469,965 - +1.3 469,965 - +1.2 470,079 - mdlB b0449 0.12 +1.0 470,079 - mdlB b0449 0.12 +1.8 470,166 + mdlB b0449 0.17 +1.6 470,189 - mdlB b0449 0.18 +1.3 470,259 - mdlB b0449 0.22 +1.7 470,391 + mdlB b0449 0.30 +1.1 470,406 - mdlB b0449 0.31 +0.3 470,450 + mdlB b0449 0.33 +1.3 470,575 - mdlB b0449 0.40 +1.5 470,575 - mdlB b0449 0.40 +1.5 470,582 + mdlB b0449 0.41 +1.6 470,667 + mdlB b0449 0.45 +0.8 470,694 + mdlB b0449 0.47 +0.3 470,780 + mdlB b0449 0.52 +1.2 470,788 - mdlB b0449 0.52 +0.9 470,788 - mdlB b0449 0.52 +0.7 470,805 - mdlB b0449 0.53 +1.1 470,838 + mdlB b0449 0.55 +0.8 470,838 + mdlB b0449 0.55 +1.6 471,033 - mdlB b0449 0.66 +1.0 471,033 - mdlB b0449 0.66 +1.3 471,076 - mdlB b0449 0.68 +1.6 471,076 - mdlB b0449 0.68 +0.9 471,119 - mdlB b0449 0.71 +1.7 471,119 - mdlB b0449 0.71 +1.8 471,135 + mdlB b0449 0.72 +0.9 471,224 + mdlB b0449 0.77 +1.3 471,410 - mdlB b0449 0.87 +1.3 471,433 + mdlB b0449 0.88 +1.4 471,443 + mdlB b0449 0.89 +0.3 471,447 - mdlB b0449 0.89 +2.0 471,510 - +1.0 471,522 - +1.2 471,549 + +1.0 471,551 + -0.8 471,551 + -0.3 471,679 + -1.5 471,692 - +0.8 471,699 - +0.1 471,866 + glnK b0450 0.13 +0.9 471,866 + glnK b0450 0.13 +0.7 471,884 - glnK b0450 0.18 -1.4 472,069 - glnK b0450 0.73 -0.2 472,140 + -1.5 472,180 - -0.6 472,254 - +1.7 472,257 + +0.2 472,345 + amtB b0451 0.12 -1.3 472,345 + amtB b0451 0.12 -1.0 472,415 - amtB b0451 0.17 -0.8 472,417 + amtB b0451 0.18 -0.2 472,430 - amtB b0451 0.19 +0.8 472,444 - amtB b0451 0.20 +1.7 472,546 + amtB b0451 0.28 -0.1
Or see this region's nucleotide sequence