Strain Fitness in Escherichia coli BW25113 around b0448

Experiment: Trimethoprim 0.00125 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Trimethoprim 0.00125 mM
467,096	+	cof	b0446	0.56	+0.7
467,106	+	cof	b0446	0.57	+0.0
467,209	+	cof	b0446	0.70	-0.1
467,209	+	cof	b0446	0.70	+0.5
467,226	-	cof	b0446	0.72	-0.4
467,250	+	cof	b0446	0.75	-0.1
467,260	+	cof	b0446	0.76	-0.9
467,260	+	cof	b0446	0.76	-0.1
467,268	-	cof	b0446	0.77	-0.7
467,335	+	cof	b0446	0.85	+0.2
467,350	+	cof	b0446	0.87	-0.3
467,354	-	cof	b0446	0.88	+0.8
467,354	-	cof	b0446	0.88	-0.7
467,426	+				-1.4
467,426	+				+0.6
467,454	+				+0.9
467,454	+				+0.0
467,472	-				-0.4
467,472	-				+0.1
467,472	-				-0.5
467,532	-				+0.1
467,646	+				-0.8
467,663	+	ybaO	b0447	0.12	-0.9
467,663	+	ybaO	b0447	0.12	-0.5
467,673	-	ybaO	b0447	0.14	+1.1
467,851	-	ybaO	b0447	0.53	+0.8
467,851	-	ybaO	b0447	0.53	-0.0
467,910	+	ybaO	b0447	0.66	-1.8
467,910	+	ybaO	b0447	0.66	-0.4
467,910	-	ybaO	b0447	0.66	+0.4
467,918	-	ybaO	b0447	0.68	+0.4
467,929	+	ybaO	b0447	0.70	-0.9
467,929	+	ybaO	b0447	0.70	+0.7
467,951	-	ybaO	b0447	0.75	+0.1
467,976	+	ybaO	b0447	0.80	-0.2
468,054	-				-1.3
468,129	-				+0.7
468,187	+				+1.6
468,226	-				+1.5
468,231	+				+1.2
468,252	-				+1.4
468,356	-	mdlA	b0448	0.15	+0.8
468,397	-	mdlA	b0448	0.17	+1.0
468,448	-	mdlA	b0448	0.20	+1.7
468,516	-	mdlA	b0448	0.24	+0.6
468,529	-	mdlA	b0448	0.24	+0.8
468,664	+	mdlA	b0448	0.32	+1.1
468,829	-	mdlA	b0448	0.41	+1.6
468,893	+	mdlA	b0448	0.45	+1.3
468,901	+	mdlA	b0448	0.45	+1.0
468,901	-	mdlA	b0448	0.45	+1.1
468,914	-	mdlA	b0448	0.46	+1.0
468,914	-	mdlA	b0448	0.46	-0.0
468,914	-	mdlA	b0448	0.46	+0.5
468,914	-	mdlA	b0448	0.46	+2.3
468,923	-	mdlA	b0448	0.47	+0.9
468,930	+	mdlA	b0448	0.47	+1.1
468,942	+	mdlA	b0448	0.48	+1.3
468,950	-	mdlA	b0448	0.48	+0.7
469,008	+	mdlA	b0448	0.51	+1.8
469,011	-	mdlA	b0448	0.52	+1.3
469,117	+	mdlA	b0448	0.58	+1.1
469,123	-	mdlA	b0448	0.58	+1.9
469,123	-	mdlA	b0448	0.58	+0.7
469,284	+	mdlA	b0448	0.67	+1.0
469,288	-	mdlA	b0448	0.67	+1.1
469,385	+	mdlA	b0448	0.73	+0.4
469,385	+	mdlA	b0448	0.73	+0.8
469,475	+	mdlA	b0448	0.78	+0.6
469,509	-	mdlA	b0448	0.80	+0.6
469,509	-	mdlA	b0448	0.80	+0.7
469,526	+	mdlA	b0448	0.81	+1.5
469,526	+	mdlA	b0448	0.81	+1.7
469,643	-	mdlA	b0448	0.87	+0.7
469,652	-	mdlA	b0448	0.88	+0.5
469,677	+	mdlA	b0448	0.89	+1.5
469,677	+	mdlA	b0448	0.89	+0.6
469,686	+	mdlA	b0448	0.90	+1.2
469,772	-				+1.2
469,777	-				+2.1
469,803	-				-0.2
469,830	-				+0.9
469,830	-				+2.4
469,887	+				+0.6
469,887	+				+0.3
469,921	+				+1.5
469,921	+				+1.9
469,955	-				+1.2
469,965	-				+2.2
469,965	-				+1.6
469,965	-				+1.3
469,965	-				+1.2
470,079	-	mdlB	b0449	0.12	+1.0
470,079	-	mdlB	b0449	0.12	+1.8
470,166	+	mdlB	b0449	0.17	+1.6
470,189	-	mdlB	b0449	0.18	+1.3
470,259	-	mdlB	b0449	0.22	+1.7
470,391	+	mdlB	b0449	0.30	+1.1
470,406	-	mdlB	b0449	0.31	+0.3
470,450	+	mdlB	b0449	0.33	+1.3
470,575	-	mdlB	b0449	0.40	+1.5
470,575	-	mdlB	b0449	0.40	+1.5
470,582	+	mdlB	b0449	0.41	+1.6
470,667	+	mdlB	b0449	0.45	+0.8
470,694	+	mdlB	b0449	0.47	+0.3
470,780	+	mdlB	b0449	0.52	+1.2
470,788	-	mdlB	b0449	0.52	+0.9
470,788	-	mdlB	b0449	0.52	+0.7
470,805	-	mdlB	b0449	0.53	+1.1
470,838	+	mdlB	b0449	0.55	+0.8
470,838	+	mdlB	b0449	0.55	+1.6