Experiment: Trimethoprim 0.00125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lon and hupB are separated by 208 nucleotides hupB and ppiD are separated by 191 nucleotides
b0439: lon - DNA-binding ATP-dependent protease La (NCBI), at 458,112 to 460,466
lon
b0440: hupB - HU, DNA-binding transcriptional regulator, beta subunit (NCBI), at 460,675 to 460,947
hupB
b0441: ppiD - peptidyl-prolyl cis-trans isomerase (rotamase D) (NCBI), at 461,139 to 463,010
ppiD
Position (kb) 460
461 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 459.718 kb on + strand, within lon at 459.726 kb on - strand, within lon at 459.726 kb on - strand, within lon at 459.726 kb on - strand, within lon at 459.729 kb on + strand, within lon at 459.746 kb on - strand, within lon at 459.834 kb on - strand, within lon at 460.032 kb on + strand, within lon at 460.083 kb on - strand, within lon at 460.153 kb on + strand, within lon at 460.161 kb on - strand, within lon at 460.163 kb on + strand, within lon at 460.194 kb on - strand, within lon at 460.254 kb on - strand at 460.320 kb on - strand at 460.339 kb on + strand at 460.397 kb on - strand at 460.448 kb on - strand at 460.448 kb on - strand at 460.496 kb on - strand at 460.544 kb on + strand at 460.583 kb on + strand at 460.583 kb on + strand at 460.592 kb on - strand at 460.592 kb on - strand at 460.605 kb on + strand at 460.605 kb on + strand at 460.612 kb on - strand at 460.613 kb on - strand at 460.627 kb on + strand at 460.648 kb on + strand at 460.648 kb on + strand at 460.682 kb on + strand at 460.722 kb on + strand, within hupB at 460.722 kb on + strand, within hupB at 460.722 kb on + strand, within hupB at 460.730 kb on - strand, within hupB at 460.730 kb on - strand, within hupB at 460.796 kb on + strand, within hupB at 460.804 kb on - strand, within hupB at 460.804 kb on - strand, within hupB at 460.836 kb on - strand, within hupB at 460.894 kb on + strand, within hupB at 460.911 kb on - strand, within hupB at 460.911 kb on - strand, within hupB at 460.925 kb on - strand at 460.925 kb on - strand at 460.948 kb on - strand at 461.025 kb on + strand at 461.034 kb on + strand at 461.114 kb on + strand at 461.137 kb on - strand at 461.143 kb on - strand at 461.147 kb on - strand at 461.160 kb on + strand at 461.243 kb on - strand at 461.285 kb on + strand at 461.318 kb on - strand at 461.318 kb on - strand at 461.409 kb on - strand, within ppiD at 461.438 kb on + strand, within ppiD at 461.438 kb on + strand, within ppiD at 461.444 kb on - strand, within ppiD at 461.447 kb on + strand, within ppiD at 461.493 kb on - strand, within ppiD at 461.523 kb on - strand, within ppiD at 461.581 kb on + strand, within ppiD at 461.586 kb on + strand at 461.633 kb on + strand, within ppiD at 461.633 kb on + strand, within ppiD at 461.707 kb on + strand, within ppiD at 461.769 kb on + strand, within ppiD at 461.846 kb on - strand, within ppiD at 461.900 kb on - strand, within ppiD at 461.900 kb on - strand, within ppiD at 461.900 kb on - strand, within ppiD at 461.920 kb on - strand, within ppiD at 461.939 kb on + strand, within ppiD at 461.939 kb on + strand, within ppiD at 461.941 kb on - strand, within ppiD at 461.941 kb on - strand, within ppiD
Per-strain Table
Position Strand Gene LocusTag Fraction Trimethoprim 0.00125 mM remove 459,718 + lon b0439 0.68 +3.9 459,726 - lon b0439 0.69 +2.5 459,726 - lon b0439 0.69 +3.4 459,726 - lon b0439 0.69 +3.1 459,729 + lon b0439 0.69 +4.1 459,746 - lon b0439 0.69 +2.9 459,834 - lon b0439 0.73 +2.0 460,032 + lon b0439 0.82 +3.5 460,083 - lon b0439 0.84 +2.7 460,153 + lon b0439 0.87 +4.0 460,161 - lon b0439 0.87 +1.7 460,163 + lon b0439 0.87 +3.3 460,194 - lon b0439 0.88 +1.4 460,254 - +3.7 460,320 - +4.7 460,339 + +2.9 460,397 - +3.5 460,448 - +3.8 460,448 - +3.5 460,496 - +3.0 460,544 + -0.6 460,583 + +1.4 460,583 + +2.4 460,592 - +1.2 460,592 - +0.1 460,605 + +0.8 460,605 + -0.4 460,612 - +0.1 460,613 - +0.2 460,627 + -0.3 460,648 + +1.3 460,648 + +1.4 460,682 + +0.6 460,722 + hupB b0440 0.17 -1.0 460,722 + hupB b0440 0.17 +1.1 460,722 + hupB b0440 0.17 +0.6 460,730 - hupB b0440 0.20 +0.2 460,730 - hupB b0440 0.20 +0.2 460,796 + hupB b0440 0.44 -0.2 460,804 - hupB b0440 0.47 +1.1 460,804 - hupB b0440 0.47 +1.0 460,836 - hupB b0440 0.59 +0.1 460,894 + hupB b0440 0.80 +1.0 460,911 - hupB b0440 0.86 +1.3 460,911 - hupB b0440 0.86 +0.8 460,925 - +1.2 460,925 - -0.0 460,948 - -0.2 461,025 + +0.2 461,034 + +0.1 461,114 + +0.3 461,137 - -0.8 461,143 - +0.2 461,147 - +0.5 461,160 + +0.4 461,243 - +2.3 461,285 + -0.5 461,318 - -0.1 461,318 - +0.4 461,409 - ppiD b0441 0.14 -0.1 461,438 + ppiD b0441 0.16 -0.8 461,438 + ppiD b0441 0.16 -0.4 461,444 - ppiD b0441 0.16 +0.8 461,447 + ppiD b0441 0.16 -1.1 461,493 - ppiD b0441 0.19 -0.1 461,523 - ppiD b0441 0.21 +1.3 461,581 + ppiD b0441 0.24 +0.4 461,586 + -0.9 461,633 + ppiD b0441 0.26 -1.7 461,633 + ppiD b0441 0.26 -0.9 461,707 + ppiD b0441 0.30 -0.9 461,769 + ppiD b0441 0.34 +0.7 461,846 - ppiD b0441 0.38 -0.2 461,900 - ppiD b0441 0.41 +0.5 461,900 - ppiD b0441 0.41 -1.2 461,900 - ppiD b0441 0.41 -0.8 461,920 - ppiD b0441 0.42 +0.2 461,939 + ppiD b0441 0.43 -1.5 461,939 + ppiD b0441 0.43 -0.8 461,941 - ppiD b0441 0.43 -0.1 461,941 - ppiD b0441 0.43 +0.5
Or see this region's nucleotide sequence