Experiment: Trimethoprim 0.00125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phoE and proB are separated by 287 nucleotides proB and proA are separated by 11 nucleotides
b0241: phoE - outer membrane phosphoporin protein E (NCBI), at 258,269 to 259,324
phoE
b0242: proB - gamma-glutamyl kinase (NCBI), at 259,612 to 260,715
proB
b0243: proA - gamma-glutamyl phosphate reductase (NCBI), at 260,727 to 261,980
proA
Position (kb) 259
260
261 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 258.625 kb on - strand, within phoE at 258.630 kb on + strand, within phoE at 258.630 kb on + strand, within phoE at 258.630 kb on + strand, within phoE at 258.630 kb on - strand, within phoE at 258.630 kb on - strand, within phoE at 258.637 kb on + strand, within phoE at 258.638 kb on - strand, within phoE at 258.638 kb on - strand, within phoE at 258.639 kb on + strand, within phoE at 258.649 kb on + strand, within phoE at 258.657 kb on - strand, within phoE at 258.657 kb on - strand, within phoE at 258.659 kb on - strand, within phoE at 258.660 kb on - strand, within phoE at 258.660 kb on - strand, within phoE at 258.662 kb on + strand, within phoE at 258.662 kb on + strand, within phoE at 258.662 kb on + strand, within phoE at 258.670 kb on - strand, within phoE at 258.670 kb on - strand, within phoE at 258.670 kb on - strand, within phoE at 258.670 kb on - strand, within phoE at 258.674 kb on - strand, within phoE at 258.682 kb on - strand, within phoE at 258.699 kb on + strand, within phoE at 258.702 kb on - strand, within phoE at 258.702 kb on - strand, within phoE at 258.702 kb on - strand, within phoE at 258.712 kb on - strand, within phoE at 258.737 kb on + strand, within phoE at 258.737 kb on + strand, within phoE at 258.753 kb on - strand, within phoE at 258.851 kb on + strand, within phoE at 258.851 kb on + strand, within phoE at 258.899 kb on - strand, within phoE at 258.899 kb on - strand, within phoE at 258.905 kb on + strand, within phoE at 258.962 kb on + strand, within phoE at 258.971 kb on - strand, within phoE at 258.973 kb on + strand, within phoE at 258.973 kb on + strand, within phoE at 258.973 kb on + strand, within phoE at 259.041 kb on + strand, within phoE at 259.056 kb on + strand, within phoE at 259.059 kb on - strand, within phoE at 259.064 kb on - strand, within phoE at 259.087 kb on + strand, within phoE at 259.087 kb on + strand, within phoE at 259.089 kb on + strand, within phoE at 259.090 kb on - strand, within phoE at 259.106 kb on + strand, within phoE at 259.115 kb on - strand, within phoE at 259.115 kb on - strand, within phoE at 259.183 kb on + strand, within phoE at 259.213 kb on - strand, within phoE at 259.213 kb on - strand, within phoE at 259.214 kb on - strand, within phoE at 259.270 kb on - strand at 259.270 kb on - strand at 259.271 kb on - strand at 259.271 kb on - strand at 259.277 kb on + strand at 259.280 kb on - strand at 259.290 kb on - strand at 259.299 kb on + strand at 259.299 kb on + strand at 259.299 kb on + strand at 259.353 kb on + strand at 259.357 kb on + strand at 259.461 kb on - strand at 259.461 kb on - strand at 259.493 kb on - strand at 259.499 kb on - strand at 259.499 kb on - strand at 259.503 kb on - strand at 259.543 kb on - strand at 259.546 kb on - strand at 259.563 kb on - strand at 259.584 kb on - strand at 259.660 kb on - strand at 259.660 kb on - strand at 259.675 kb on + strand at 259.694 kb on - strand at 259.720 kb on + strand at 259.720 kb on + strand at 259.722 kb on + strand at 259.722 kb on + strand at 259.804 kb on + strand, within proB at 259.851 kb on - strand, within proB at 260.024 kb on - strand, within proB at 260.048 kb on - strand, within proB at 260.138 kb on + strand, within proB at 260.243 kb on - strand, within proB at 260.279 kb on + strand, within proB at 260.378 kb on - strand, within proB at 260.378 kb on - strand, within proB at 260.506 kb on + strand, within proB at 260.506 kb on + strand, within proB at 260.569 kb on - strand, within proB at 260.594 kb on + strand, within proB at 260.609 kb on + strand at 260.611 kb on + strand at 260.625 kb on - strand at 260.629 kb on - strand at 260.666 kb on + strand at 260.666 kb on + strand at 260.760 kb on + strand at 260.852 kb on + strand at 260.932 kb on + strand, within proA at 260.932 kb on + strand, within proA at 260.932 kb on - strand, within proA at 260.932 kb on - strand, within proA at 260.981 kb on - strand, within proA at 260.983 kb on - strand, within proA at 260.983 kb on - strand, within proA at 261.176 kb on + strand, within proA at 261.266 kb on - strand at 261.266 kb on - strand, within proA at 261.360 kb on - strand, within proA at 261.360 kb on - strand, within proA at 261.388 kb on + strand, within proA at 261.439 kb on + strand, within proA at 261.449 kb on + strand, within proA at 261.449 kb on + strand, within proA at 261.476 kb on + strand, within proA at 261.476 kb on + strand, within proA at 261.583 kb on - strand, within proA at 261.583 kb on - strand, within proA at 261.605 kb on + strand, within proA at 261.656 kb on - strand, within proA at 261.670 kb on - strand, within proA at 261.670 kb on - strand, within proA
Per-strain Table
Position Strand Gene LocusTag Fraction Trimethoprim 0.00125 mM remove 258,625 - phoE b0241 0.34 -0.3 258,630 + phoE b0241 0.34 +1.7 258,630 + phoE b0241 0.34 +2.7 258,630 + phoE b0241 0.34 +0.7 258,630 - phoE b0241 0.34 -0.5 258,630 - phoE b0241 0.34 -0.9 258,637 + phoE b0241 0.35 +0.7 258,638 - phoE b0241 0.35 +0.5 258,638 - phoE b0241 0.35 -0.2 258,639 + phoE b0241 0.35 +3.3 258,649 + phoE b0241 0.36 -0.6 258,657 - phoE b0241 0.37 +0.1 258,657 - phoE b0241 0.37 +0.5 258,659 - phoE b0241 0.37 +0.1 258,660 - phoE b0241 0.37 -0.5 258,660 - phoE b0241 0.37 +0.5 258,662 + phoE b0241 0.37 +0.9 258,662 + phoE b0241 0.37 -0.3 258,662 + phoE b0241 0.37 -0.6 258,670 - phoE b0241 0.38 +0.1 258,670 - phoE b0241 0.38 -0.9 258,670 - phoE b0241 0.38 -1.5 258,670 - phoE b0241 0.38 -1.1 258,674 - phoE b0241 0.38 +0.2 258,682 - phoE b0241 0.39 -0.4 258,699 + phoE b0241 0.41 -0.7 258,702 - phoE b0241 0.41 +0.9 258,702 - phoE b0241 0.41 -0.2 258,702 - phoE b0241 0.41 +0.7 258,712 - phoE b0241 0.42 -0.7 258,737 + phoE b0241 0.44 -0.9 258,737 + phoE b0241 0.44 -0.3 258,753 - phoE b0241 0.46 -1.1 258,851 + phoE b0241 0.55 +0.7 258,851 + phoE b0241 0.55 -0.6 258,899 - phoE b0241 0.60 -0.7 258,899 - phoE b0241 0.60 +0.6 258,905 + phoE b0241 0.60 -0.9 258,962 + phoE b0241 0.66 +0.7 258,971 - phoE b0241 0.66 -1.8 258,973 + phoE b0241 0.67 +0.7 258,973 + phoE b0241 0.67 -0.3 258,973 + phoE b0241 0.67 -0.9 259,041 + phoE b0241 0.73 +1.7 259,056 + phoE b0241 0.75 +1.7 259,059 - phoE b0241 0.75 +1.7 259,064 - phoE b0241 0.75 -0.9 259,087 + phoE b0241 0.77 +1.7 259,087 + phoE b0241 0.77 +2.7 259,089 + phoE b0241 0.78 +1.7 259,090 - phoE b0241 0.78 +1.7 259,106 + phoE b0241 0.79 -0.3 259,115 - phoE b0241 0.80 -2.1 259,115 - phoE b0241 0.80 +1.1 259,183 + phoE b0241 0.87 +1.7 259,213 - phoE b0241 0.89 -1.1 259,213 - phoE b0241 0.89 -0.9 259,214 - phoE b0241 0.89 -0.0 259,270 - +1.3 259,270 - +2.7 259,271 - +0.7 259,271 - +1.7 259,277 + +1.7 259,280 - +0.7 259,290 - +1.7 259,299 + +1.7 259,299 + +1.7 259,299 + +0.7 259,353 + +1.7 259,357 + +0.1 259,461 - -2.2 259,461 - -1.0 259,493 - -0.4 259,499 - -0.5 259,499 - -0.5 259,503 - -1.1 259,543 - -0.5 259,546 - +0.2 259,563 - +0.3 259,584 - -0.2 259,660 - +0.9 259,660 - +0.1 259,675 + -0.0 259,694 - +1.0 259,720 + -1.4 259,720 + -0.9 259,722 + -0.4 259,722 + +0.7 259,804 + proB b0242 0.17 +1.1 259,851 - proB b0242 0.22 -0.1 260,024 - proB b0242 0.37 +1.2 260,048 - proB b0242 0.39 +1.0 260,138 + proB b0242 0.48 +1.2 260,243 - proB b0242 0.57 +0.6 260,279 + proB b0242 0.60 +0.9 260,378 - proB b0242 0.69 +1.9 260,378 - proB b0242 0.69 -1.5 260,506 + proB b0242 0.81 +0.7 260,506 + proB b0242 0.81 -1.0 260,569 - proB b0242 0.87 +1.7 260,594 + proB b0242 0.89 -0.2 260,609 + +0.1 260,611 + +0.6 260,625 - +1.1 260,629 - +1.4 260,666 + +0.7 260,666 + +1.1 260,760 + +0.3 260,852 + -0.8 260,932 + proA b0243 0.16 -0.9 260,932 + proA b0243 0.16 -0.4 260,932 - proA b0243 0.16 +0.3 260,932 - proA b0243 0.16 +0.4 260,981 - proA b0243 0.20 +0.7 260,983 - proA b0243 0.20 +0.7 260,983 - proA b0243 0.20 -1.0 261,176 + proA b0243 0.36 +0.1 261,266 - +1.7 261,266 - proA b0243 0.43 +1.7 261,360 - proA b0243 0.50 +0.3 261,360 - proA b0243 0.50 +0.1 261,388 + proA b0243 0.53 +0.0 261,439 + proA b0243 0.57 -1.4 261,449 + proA b0243 0.58 +0.4 261,449 + proA b0243 0.58 -0.3 261,476 + proA b0243 0.60 +1.1 261,476 + proA b0243 0.60 +1.1 261,583 - proA b0243 0.68 -0.4 261,583 - proA b0243 0.68 +0.5 261,605 + proA b0243 0.70 +1.7 261,656 - proA b0243 0.74 +0.4 261,670 - proA b0243 0.75 -0.4 261,670 - proA b0243 0.75 +0.5
Or see this region's nucleotide sequence