Strain Fitness in Escherichia coli BW25113 around b0176

Experiment: Trimethoprim 0.00125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntispU and cdsA are separated by 12 nucleotidescdsA and yaeL are separated by 11 nucleotidesyaeL and yaeT are separated by 29 nucleotides b0174: ispU - undecaprenyl pyrophosphate synthase (NCBI), at 194,903 to 195,664 ispU b0175: cdsA - CDP-diglyceride synthetase (VIMSS), at 195,677 to 196,534 cdsA b0176: yaeL - zinc metallopeptidase (NCBI), at 196,546 to 197,898 yaeL b0177: yaeT - hypothetical protein (NCBI), at 197,928 to 200,360 yaeT Position (kb) 196 197 198Strain fitness (log2 ratio) -2 -1 0 1 2at 195.682 kb on + strandat 197.891 kb on + strandat 197.891 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.00125 mM
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195,682 + -2.5
197,891 + +2.0
197,891 + +1.6

Or see this region's nucleotide sequence