Experiment: Ciprofloxacin Hydrochloride 0.0003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rbsA and rbsC are separated by 4 nucleotides rbsC and rbsB are separated by 24 nucleotides rbsB and rbsK are separated by 125 nucleotides
b3749: rbsA - fused D-ribose transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 3,931,801 to 3,933,306
rbsA
b3750: rbsC - ribose ABC transporter permease protein (NCBI), at 3,933,311 to 3,934,276
rbsC
b3751: rbsB - D-ribose transporter subunit (NCBI), at 3,934,301 to 3,935,191
rbsB
b3752: rbsK - ribokinase (NCBI), at 3,935,317 to 3,936,246
rbsK
Position (kb)
3934
3935
3936 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3933.317 kb on + strand at 3933.317 kb on + strand at 3933.324 kb on - strand at 3933.345 kb on - strand at 3933.345 kb on - strand at 3933.384 kb on + strand at 3933.460 kb on + strand, within rbsC at 3933.481 kb on - strand, within rbsC at 3933.495 kb on + strand, within rbsC at 3933.495 kb on + strand, within rbsC at 3933.540 kb on + strand, within rbsC at 3933.548 kb on - strand, within rbsC at 3933.548 kb on - strand, within rbsC at 3933.592 kb on + strand, within rbsC at 3933.592 kb on + strand, within rbsC at 3933.755 kb on + strand, within rbsC at 3933.755 kb on + strand, within rbsC at 3933.768 kb on + strand, within rbsC at 3933.815 kb on - strand, within rbsC at 3933.815 kb on - strand, within rbsC at 3933.848 kb on - strand, within rbsC at 3933.856 kb on + strand, within rbsC at 3933.866 kb on + strand, within rbsC at 3933.876 kb on - strand, within rbsC at 3933.878 kb on - strand, within rbsC at 3933.883 kb on - strand, within rbsC at 3933.883 kb on - strand, within rbsC at 3933.886 kb on - strand, within rbsC at 3933.890 kb on + strand, within rbsC at 3933.898 kb on + strand, within rbsC at 3933.898 kb on + strand, within rbsC at 3933.898 kb on - strand, within rbsC at 3933.924 kb on - strand, within rbsC at 3933.924 kb on - strand, within rbsC at 3933.937 kb on + strand, within rbsC at 3933.950 kb on + strand, within rbsC at 3933.950 kb on + strand, within rbsC at 3934.001 kb on - strand, within rbsC at 3934.001 kb on - strand, within rbsC at 3934.003 kb on - strand, within rbsC at 3934.051 kb on - strand, within rbsC at 3934.056 kb on - strand, within rbsC at 3934.075 kb on + strand at 3934.094 kb on + strand, within rbsC at 3934.097 kb on - strand, within rbsC at 3934.101 kb on - strand, within rbsC at 3934.110 kb on - strand, within rbsC at 3934.269 kb on - strand at 3934.323 kb on + strand at 3934.323 kb on + strand at 3934.337 kb on + strand at 3934.383 kb on + strand at 3934.397 kb on - strand, within rbsB at 3934.467 kb on + strand, within rbsB at 3934.478 kb on + strand, within rbsB at 3934.489 kb on - strand, within rbsB at 3934.490 kb on + strand, within rbsB at 3934.528 kb on + strand, within rbsB at 3934.594 kb on - strand, within rbsB at 3934.594 kb on - strand, within rbsB at 3934.643 kb on - strand, within rbsB at 3934.684 kb on + strand, within rbsB at 3934.684 kb on + strand, within rbsB at 3934.687 kb on - strand, within rbsB at 3934.698 kb on - strand, within rbsB at 3934.698 kb on - strand, within rbsB at 3934.714 kb on + strand, within rbsB at 3934.714 kb on + strand, within rbsB at 3934.722 kb on - strand, within rbsB at 3934.751 kb on - strand, within rbsB at 3934.767 kb on + strand, within rbsB at 3934.774 kb on + strand, within rbsB at 3934.781 kb on + strand, within rbsB at 3934.842 kb on - strand, within rbsB at 3934.873 kb on + strand, within rbsB at 3934.881 kb on - strand, within rbsB at 3934.881 kb on - strand, within rbsB at 3934.908 kb on - strand, within rbsB at 3934.927 kb on + strand, within rbsB at 3934.930 kb on - strand, within rbsB at 3934.930 kb on - strand, within rbsB at 3934.931 kb on + strand, within rbsB at 3934.940 kb on - strand, within rbsB at 3934.950 kb on - strand, within rbsB at 3935.013 kb on + strand, within rbsB at 3935.022 kb on + strand, within rbsB at 3935.144 kb on + strand at 3935.155 kb on + strand at 3935.163 kb on - strand at 3935.201 kb on + strand at 3935.212 kb on - strand at 3935.222 kb on + strand at 3935.280 kb on + strand at 3935.420 kb on + strand, within rbsK at 3935.420 kb on + strand, within rbsK at 3935.469 kb on - strand, within rbsK at 3935.485 kb on - strand, within rbsK at 3935.493 kb on - strand, within rbsK at 3935.517 kb on - strand, within rbsK at 3935.517 kb on - strand, within rbsK at 3935.519 kb on + strand, within rbsK at 3935.519 kb on + strand, within rbsK at 3935.522 kb on - strand, within rbsK at 3935.522 kb on - strand, within rbsK at 3935.542 kb on - strand, within rbsK at 3935.542 kb on - strand, within rbsK at 3935.551 kb on + strand, within rbsK at 3935.551 kb on + strand, within rbsK at 3935.560 kb on - strand, within rbsK at 3935.577 kb on - strand, within rbsK at 3935.650 kb on + strand, within rbsK at 3935.656 kb on - strand, within rbsK at 3935.661 kb on + strand, within rbsK at 3935.666 kb on + strand, within rbsK at 3935.669 kb on - strand, within rbsK at 3935.669 kb on - strand, within rbsK at 3935.669 kb on - strand, within rbsK at 3935.711 kb on + strand, within rbsK at 3935.742 kb on + strand, within rbsK at 3935.798 kb on - strand, within rbsK at 3935.798 kb on - strand, within rbsK at 3935.808 kb on + strand, within rbsK at 3935.942 kb on + strand, within rbsK at 3935.946 kb on + strand, within rbsK at 3935.946 kb on + strand, within rbsK at 3935.955 kb on - strand, within rbsK at 3935.969 kb on + strand, within rbsK at 3935.969 kb on + strand, within rbsK at 3935.977 kb on - strand, within rbsK at 3935.977 kb on - strand, within rbsK at 3935.983 kb on + strand, within rbsK at 3936.007 kb on - strand, within rbsK at 3936.007 kb on - strand, within rbsK at 3936.007 kb on - strand, within rbsK at 3936.009 kb on - strand, within rbsK at 3936.028 kb on - strand, within rbsK at 3936.028 kb on - strand, within rbsK at 3936.048 kb on + strand, within rbsK at 3936.055 kb on - strand, within rbsK at 3936.074 kb on - strand, within rbsK at 3936.074 kb on - strand, within rbsK at 3936.082 kb on + strand, within rbsK at 3936.082 kb on + strand, within rbsK
Per-strain Table
Position Strand Gene LocusTag Fraction Ciprofloxacin Hydrochloride 0.0003 mM remove 3,933,317 + +0.3 3,933,317 + +0.4 3,933,324 - +0.2 3,933,345 - +0.3 3,933,345 - +0.3 3,933,384 + +0.6 3,933,460 + rbsC b3750 0.15 +0.6 3,933,481 - rbsC b3750 0.18 -0.8 3,933,495 + rbsC b3750 0.19 +0.9 3,933,495 + rbsC b3750 0.19 +0.5 3,933,540 + rbsC b3750 0.24 +0.8 3,933,548 - rbsC b3750 0.25 -0.1 3,933,548 - rbsC b3750 0.25 -0.3 3,933,592 + rbsC b3750 0.29 +2.0 3,933,592 + rbsC b3750 0.29 +0.2 3,933,755 + rbsC b3750 0.46 -0.1 3,933,755 + rbsC b3750 0.46 -0.7 3,933,768 + rbsC b3750 0.47 +1.3 3,933,815 - rbsC b3750 0.52 -1.1 3,933,815 - rbsC b3750 0.52 -0.4 3,933,848 - rbsC b3750 0.56 -1.8 3,933,856 + rbsC b3750 0.56 +1.4 3,933,866 + rbsC b3750 0.57 -0.3 3,933,876 - rbsC b3750 0.58 +0.7 3,933,878 - rbsC b3750 0.59 +0.6 3,933,883 - rbsC b3750 0.59 -0.2 3,933,883 - rbsC b3750 0.59 +0.3 3,933,886 - rbsC b3750 0.60 -0.6 3,933,890 + rbsC b3750 0.60 +0.8 3,933,898 + rbsC b3750 0.61 +0.9 3,933,898 + rbsC b3750 0.61 +1.8 3,933,898 - rbsC b3750 0.61 -0.2 3,933,924 - rbsC b3750 0.63 -0.3 3,933,924 - rbsC b3750 0.63 -0.3 3,933,937 + rbsC b3750 0.65 +2.9 3,933,950 + rbsC b3750 0.66 +2.2 3,933,950 + rbsC b3750 0.66 +1.4 3,934,001 - rbsC b3750 0.71 -0.2 3,934,001 - rbsC b3750 0.71 -0.2 3,934,003 - rbsC b3750 0.72 -0.7 3,934,051 - rbsC b3750 0.77 +0.3 3,934,056 - rbsC b3750 0.77 +1.6 3,934,075 + +1.0 3,934,094 + rbsC b3750 0.81 +0.8 3,934,097 - rbsC b3750 0.81 -1.3 3,934,101 - rbsC b3750 0.82 -0.2 3,934,110 - rbsC b3750 0.83 -0.3 3,934,269 - -0.0 3,934,323 + +0.7 3,934,323 + +0.3 3,934,337 + +1.2 3,934,383 + +1.2 3,934,397 - rbsB b3751 0.11 -0.5 3,934,467 + rbsB b3751 0.19 +0.6 3,934,478 + rbsB b3751 0.20 +1.5 3,934,489 - rbsB b3751 0.21 +0.1 3,934,490 + rbsB b3751 0.21 +1.9 3,934,528 + rbsB b3751 0.25 +1.7 3,934,594 - rbsB b3751 0.33 +0.3 3,934,594 - rbsB b3751 0.33 -3.7 3,934,643 - rbsB b3751 0.38 +0.3 3,934,684 + rbsB b3751 0.43 +1.1 3,934,684 + rbsB b3751 0.43 +0.7 3,934,687 - rbsB b3751 0.43 -0.1 3,934,698 - rbsB b3751 0.45 +0.1 3,934,698 - rbsB b3751 0.45 -0.4 3,934,714 + rbsB b3751 0.46 +1.7 3,934,714 + rbsB b3751 0.46 +1.0 3,934,722 - rbsB b3751 0.47 -1.3 3,934,751 - rbsB b3751 0.51 -1.4 3,934,767 + rbsB b3751 0.52 +1.8 3,934,774 + rbsB b3751 0.53 +1.6 3,934,781 + rbsB b3751 0.54 +2.3 3,934,842 - rbsB b3751 0.61 +1.5 3,934,873 + rbsB b3751 0.64 +1.5 3,934,881 - rbsB b3751 0.65 -0.1 3,934,881 - rbsB b3751 0.65 +0.6 3,934,908 - rbsB b3751 0.68 +0.2 3,934,927 + rbsB b3751 0.70 +2.0 3,934,930 - rbsB b3751 0.71 -0.1 3,934,930 - rbsB b3751 0.71 -0.3 3,934,931 + rbsB b3751 0.71 +1.8 3,934,940 - rbsB b3751 0.72 +0.2 3,934,950 - rbsB b3751 0.73 +1.5 3,935,013 + rbsB b3751 0.80 +1.1 3,935,022 + rbsB b3751 0.81 +3.0 3,935,144 + +2.1 3,935,155 + +2.4 3,935,163 - +0.6 3,935,201 + +1.0 3,935,212 - +1.3 3,935,222 + +0.9 3,935,280 + +2.4 3,935,420 + rbsK b3752 0.11 +0.5 3,935,420 + rbsK b3752 0.11 -3.1 3,935,469 - rbsK b3752 0.16 -0.9 3,935,485 - rbsK b3752 0.18 -1.0 3,935,493 - rbsK b3752 0.19 +0.4 3,935,517 - rbsK b3752 0.22 -2.3 3,935,517 - rbsK b3752 0.22 -0.8 3,935,519 + rbsK b3752 0.22 +0.7 3,935,519 + rbsK b3752 0.22 +0.5 3,935,522 - rbsK b3752 0.22 -1.3 3,935,522 - rbsK b3752 0.22 -1.9 3,935,542 - rbsK b3752 0.24 -0.6 3,935,542 - rbsK b3752 0.24 +0.7 3,935,551 + rbsK b3752 0.25 +0.2 3,935,551 + rbsK b3752 0.25 -0.5 3,935,560 - rbsK b3752 0.26 -0.7 3,935,577 - rbsK b3752 0.28 -0.2 3,935,650 + rbsK b3752 0.36 -0.4 3,935,656 - rbsK b3752 0.36 -1.8 3,935,661 + rbsK b3752 0.37 -1.2 3,935,666 + rbsK b3752 0.38 -1.4 3,935,669 - rbsK b3752 0.38 +1.4 3,935,669 - rbsK b3752 0.38 +0.2 3,935,669 - rbsK b3752 0.38 +0.1 3,935,711 + rbsK b3752 0.42 +0.8 3,935,742 + rbsK b3752 0.46 +1.7 3,935,798 - rbsK b3752 0.52 -0.5 3,935,798 - rbsK b3752 0.52 +0.9 3,935,808 + rbsK b3752 0.53 +0.2 3,935,942 + rbsK b3752 0.67 -0.1 3,935,946 + rbsK b3752 0.68 -0.4 3,935,946 + rbsK b3752 0.68 +0.0 3,935,955 - rbsK b3752 0.69 -1.8 3,935,969 + rbsK b3752 0.70 +1.2 3,935,969 + rbsK b3752 0.70 +0.9 3,935,977 - rbsK b3752 0.71 +0.4 3,935,977 - rbsK b3752 0.71 +0.8 3,935,983 + rbsK b3752 0.72 +0.2 3,936,007 - rbsK b3752 0.74 -0.0 3,936,007 - rbsK b3752 0.74 -1.3 3,936,007 - rbsK b3752 0.74 -1.1 3,936,009 - rbsK b3752 0.74 +0.3 3,936,028 - rbsK b3752 0.76 -2.1 3,936,028 - rbsK b3752 0.76 +0.3 3,936,048 + rbsK b3752 0.79 -0.8 3,936,055 - rbsK b3752 0.79 -0.9 3,936,074 - rbsK b3752 0.81 -0.4 3,936,074 - rbsK b3752 0.81 -0.7 3,936,082 + rbsK b3752 0.82 -1.1 3,936,082 + rbsK b3752 0.82 -1.1
Or see this region's nucleotide sequence