Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygaM and nrdH are separated by 247 nucleotides nrdH and nrdI overlap by 4 nucleotides nrdI and nrdE overlap by 28 nucleotides nrdE and nrdF are separated by 9 nucleotides
b2672: ygaM - orf, hypothetical protein (VIMSS), at 2,798,156 to 2,798,497
ygaM
b2673: nrdH - glutaredoxin-like protein (NCBI), at 2,798,745 to 2,798,990
nrdH
b2674: nrdI - hypothetical protein (NCBI), at 2,798,987 to 2,799,397
nrdI
b2675: nrdE - ribonucleotide-diphosphate reductase alpha subunit (NCBI), at 2,799,370 to 2,801,514
nrdE
b2676: nrdF - ribonucleotide-diphosphate reductase beta subunit (NCBI), at 2,801,524 to 2,802,483
nrdF
Position (kb)
2799
2800
2801
2802 Strain fitness (log2 ratio)
-2
-1
0
1 at 2798.387 kb on + strand, within ygaM at 2798.395 kb on - strand, within ygaM at 2798.427 kb on + strand, within ygaM at 2798.481 kb on - strand at 2798.481 kb on - strand at 2798.600 kb on + strand at 2798.614 kb on - strand at 2798.834 kb on + strand, within nrdH at 2798.849 kb on + strand, within nrdH at 2798.917 kb on + strand, within nrdH at 2798.917 kb on + strand, within nrdH at 2798.983 kb on + strand at 2798.983 kb on + strand at 2798.991 kb on - strand at 2799.158 kb on + strand, within nrdI at 2799.191 kb on + strand, within nrdI at 2799.251 kb on + strand, within nrdI at 2799.410 kb on - strand at 2799.505 kb on - strand at 2799.517 kb on + strand at 2799.535 kb on - strand at 2799.535 kb on - strand at 2799.535 kb on - strand at 2799.535 kb on - strand at 2799.631 kb on + strand, within nrdE at 2799.631 kb on - strand, within nrdE at 2799.756 kb on + strand, within nrdE at 2799.764 kb on - strand, within nrdE at 2799.764 kb on - strand, within nrdE at 2799.784 kb on - strand, within nrdE at 2799.784 kb on - strand, within nrdE at 2800.103 kb on - strand, within nrdE at 2800.115 kb on + strand, within nrdE at 2800.151 kb on + strand, within nrdE at 2800.168 kb on - strand, within nrdE at 2800.168 kb on - strand, within nrdE at 2800.248 kb on + strand, within nrdE at 2800.457 kb on + strand, within nrdE at 2800.457 kb on + strand, within nrdE at 2800.468 kb on + strand, within nrdE at 2800.670 kb on + strand, within nrdE at 2800.699 kb on - strand, within nrdE at 2800.699 kb on - strand, within nrdE at 2800.710 kb on - strand, within nrdE at 2800.719 kb on + strand, within nrdE at 2800.796 kb on + strand, within nrdE at 2800.826 kb on - strand, within nrdE at 2800.853 kb on + strand, within nrdE at 2800.877 kb on + strand, within nrdE at 2800.877 kb on + strand, within nrdE at 2800.877 kb on + strand, within nrdE at 2800.887 kb on + strand, within nrdE at 2800.944 kb on + strand, within nrdE at 2800.944 kb on + strand, within nrdE at 2801.039 kb on + strand, within nrdE at 2801.098 kb on + strand, within nrdE at 2801.116 kb on - strand, within nrdE at 2801.138 kb on + strand, within nrdE at 2801.138 kb on + strand, within nrdE at 2801.145 kb on + strand, within nrdE at 2801.146 kb on - strand, within nrdE at 2801.167 kb on - strand, within nrdE at 2801.226 kb on + strand, within nrdE at 2801.234 kb on + strand, within nrdE at 2801.235 kb on - strand, within nrdE at 2801.247 kb on - strand, within nrdE at 2801.247 kb on - strand, within nrdE at 2801.320 kb on + strand at 2801.421 kb on + strand at 2801.421 kb on + strand at 2801.421 kb on + strand at 2801.472 kb on + strand at 2801.480 kb on - strand at 2801.548 kb on + strand at 2801.548 kb on + strand at 2801.561 kb on + strand at 2801.587 kb on - strand at 2801.587 kb on - strand at 2801.604 kb on - strand at 2801.777 kb on + strand, within nrdF at 2801.777 kb on + strand, within nrdF at 2801.829 kb on - strand, within nrdF at 2801.895 kb on - strand, within nrdF at 2801.895 kb on - strand, within nrdF at 2802.009 kb on - strand, within nrdF at 2802.073 kb on - strand, within nrdF at 2802.119 kb on - strand, within nrdF at 2802.216 kb on - strand, within nrdF at 2802.216 kb on - strand, within nrdF at 2802.233 kb on - strand, within nrdF at 2802.293 kb on + strand, within nrdF at 2802.313 kb on + strand, within nrdF at 2802.326 kb on - strand, within nrdF at 2802.326 kb on - strand, within nrdF at 2802.422 kb on - strand at 2802.422 kb on - strand at 2802.424 kb on + strand at 2802.428 kb on + strand at 2802.428 kb on + strand at 2802.428 kb on + strand at 2802.428 kb on + strand at 2802.428 kb on + strand at 2802.431 kb on + strand at 2802.431 kb on + strand at 2802.436 kb on - strand at 2802.438 kb on + strand at 2802.455 kb on + strand at 2802.463 kb on + strand at 2802.463 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,798,387 + ygaM b2672 0.68 +0.0 2,798,395 - ygaM b2672 0.70 -0.1 2,798,427 + ygaM b2672 0.79 +0.1 2,798,481 - -0.1 2,798,481 - +0.9 2,798,600 + +0.2 2,798,614 - -0.3 2,798,834 + nrdH b2673 0.36 +1.0 2,798,849 + nrdH b2673 0.42 +0.9 2,798,917 + nrdH b2673 0.70 +0.1 2,798,917 + nrdH b2673 0.70 +0.0 2,798,983 + -0.1 2,798,983 + -1.2 2,798,991 - +0.7 2,799,158 + nrdI b2674 0.42 +0.0 2,799,191 + nrdI b2674 0.50 +0.0 2,799,251 + nrdI b2674 0.64 -0.3 2,799,410 - -0.8 2,799,505 - +0.2 2,799,517 + -0.5 2,799,535 - +0.2 2,799,535 - +0.4 2,799,535 - -0.4 2,799,535 - +0.4 2,799,631 + nrdE b2675 0.12 +0.4 2,799,631 - nrdE b2675 0.12 -1.1 2,799,756 + nrdE b2675 0.18 +0.5 2,799,764 - nrdE b2675 0.18 -0.8 2,799,764 - nrdE b2675 0.18 +0.3 2,799,784 - nrdE b2675 0.19 -0.1 2,799,784 - nrdE b2675 0.19 +0.2 2,800,103 - nrdE b2675 0.34 +0.9 2,800,115 + nrdE b2675 0.35 -1.0 2,800,151 + nrdE b2675 0.36 -0.4 2,800,168 - nrdE b2675 0.37 -0.4 2,800,168 - nrdE b2675 0.37 -1.9 2,800,248 + nrdE b2675 0.41 -0.1 2,800,457 + nrdE b2675 0.51 +0.6 2,800,457 + nrdE b2675 0.51 -0.1 2,800,468 + nrdE b2675 0.51 -0.7 2,800,670 + nrdE b2675 0.61 +0.0 2,800,699 - nrdE b2675 0.62 +0.7 2,800,699 - nrdE b2675 0.62 +0.2 2,800,710 - nrdE b2675 0.62 -0.8 2,800,719 + nrdE b2675 0.63 +0.3 2,800,796 + nrdE b2675 0.66 +0.2 2,800,826 - nrdE b2675 0.68 +0.1 2,800,853 + nrdE b2675 0.69 -0.6 2,800,877 + nrdE b2675 0.70 +0.5 2,800,877 + nrdE b2675 0.70 +0.5 2,800,877 + nrdE b2675 0.70 -0.2 2,800,887 + nrdE b2675 0.71 +0.3 2,800,944 + nrdE b2675 0.73 +0.7 2,800,944 + nrdE b2675 0.73 -0.7 2,801,039 + nrdE b2675 0.78 +0.4 2,801,098 + nrdE b2675 0.81 -0.9 2,801,116 - nrdE b2675 0.81 -0.3 2,801,138 + nrdE b2675 0.82 -0.5 2,801,138 + nrdE b2675 0.82 -0.2 2,801,145 + nrdE b2675 0.83 +0.2 2,801,146 - nrdE b2675 0.83 +0.2 2,801,167 - nrdE b2675 0.84 +0.8 2,801,226 + nrdE b2675 0.87 -0.4 2,801,234 + nrdE b2675 0.87 +1.1 2,801,235 - nrdE b2675 0.87 -0.3 2,801,247 - nrdE b2675 0.88 +0.2 2,801,247 - nrdE b2675 0.88 +0.6 2,801,320 + +0.4 2,801,421 + -0.1 2,801,421 + +0.9 2,801,421 + -0.7 2,801,472 + -0.9 2,801,480 - -0.5 2,801,548 + +0.3 2,801,548 + -0.5 2,801,561 + +0.6 2,801,587 - -1.0 2,801,587 - +0.8 2,801,604 - -0.6 2,801,777 + nrdF b2676 0.26 -0.3 2,801,777 + nrdF b2676 0.26 +0.3 2,801,829 - nrdF b2676 0.32 -0.4 2,801,895 - nrdF b2676 0.39 -0.0 2,801,895 - nrdF b2676 0.39 -0.3 2,802,009 - nrdF b2676 0.51 +0.0 2,802,073 - nrdF b2676 0.57 -0.4 2,802,119 - nrdF b2676 0.62 -0.2 2,802,216 - nrdF b2676 0.72 +0.6 2,802,216 - nrdF b2676 0.72 -0.3 2,802,233 - nrdF b2676 0.74 +0.5 2,802,293 + nrdF b2676 0.80 +0.5 2,802,313 + nrdF b2676 0.82 -0.3 2,802,326 - nrdF b2676 0.84 +0.1 2,802,326 - nrdF b2676 0.84 -0.7 2,802,422 - +0.1 2,802,422 - -0.1 2,802,424 + -0.1 2,802,428 + +0.6 2,802,428 + +0.2 2,802,428 + +0.2 2,802,428 + -0.1 2,802,428 + -0.5 2,802,431 + -0.1 2,802,431 + -1.0 2,802,436 - -0.1 2,802,438 + -0.0 2,802,455 + +0.4 2,802,463 + -0.0 2,802,463 + +0.3
Or see this region's nucleotide sequence