Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yegX and thiD are separated by 64 nucleotides thiD and thiM overlap by 4 nucleotides thiM and yohL are separated by 222 nucleotides yohL and yohM are separated by 120 nucleotides
b2102: yegX - orf, hypothetical protein (VIMSS), at 2,180,855 to 2,181,673
yegX
b2103: thiD - phosphomethylpyrimidine kinase (NCBI), at 2,181,738 to 2,182,538
thiD
b2104: thiM - hydroxyethylthiazole kinase (NCBI), at 2,182,535 to 2,183,323
thiM
b2105: yohL - hypothetical protein (NCBI), at 2,183,546 to 2,183,818
yohL
b2106: yohM - membrane protein conferring nickel and cobalt resistance (NCBI), at 2,183,939 to 2,184,763
yohM
Position (kb)
2182
2183
2184 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2181.548 kb on + strand, within yegX at 2181.628 kb on + strand at 2181.628 kb on + strand at 2181.631 kb on + strand at 2181.660 kb on + strand at 2181.678 kb on - strand at 2181.695 kb on + strand at 2181.726 kb on + strand at 2181.731 kb on - strand at 2181.749 kb on - strand at 2181.801 kb on - strand at 2181.806 kb on - strand at 2181.875 kb on + strand, within thiD at 2181.883 kb on - strand, within thiD at 2181.883 kb on - strand, within thiD at 2181.883 kb on - strand, within thiD at 2182.009 kb on + strand, within thiD at 2182.009 kb on + strand, within thiD at 2182.012 kb on + strand, within thiD at 2182.020 kb on - strand, within thiD at 2182.119 kb on - strand, within thiD at 2182.150 kb on - strand, within thiD at 2182.161 kb on + strand, within thiD at 2182.375 kb on + strand, within thiD at 2182.375 kb on + strand, within thiD at 2182.382 kb on + strand, within thiD at 2182.546 kb on - strand at 2182.646 kb on + strand, within thiM at 2183.006 kb on - strand, within thiM at 2183.006 kb on - strand, within thiM at 2183.044 kb on + strand, within thiM at 2183.246 kb on + strand at 2183.246 kb on + strand at 2183.259 kb on - strand at 2183.259 kb on - strand at 2183.389 kb on - strand at 2183.399 kb on - strand at 2183.399 kb on - strand at 2183.399 kb on - strand at 2183.495 kb on - strand at 2183.533 kb on - strand at 2183.614 kb on + strand, within yohL at 2183.614 kb on - strand, within yohL at 2183.620 kb on - strand, within yohL at 2183.621 kb on + strand, within yohL at 2183.766 kb on - strand, within yohL at 2183.814 kb on - strand at 2183.814 kb on - strand at 2183.989 kb on + strand at 2184.011 kb on + strand at 2184.014 kb on + strand at 2184.014 kb on + strand at 2184.030 kb on - strand, within yohM at 2184.141 kb on + strand, within yohM at 2184.141 kb on + strand, within yohM at 2184.272 kb on + strand, within yohM
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,181,548 + yegX b2102 0.85 +0.4 2,181,628 + -0.2 2,181,628 + +1.1 2,181,631 + -0.0 2,181,660 + -0.2 2,181,678 - -0.4 2,181,695 + -0.1 2,181,726 + -0.5 2,181,731 - -0.6 2,181,749 - +0.2 2,181,801 - +0.1 2,181,806 - +0.1 2,181,875 + thiD b2103 0.17 +0.1 2,181,883 - thiD b2103 0.18 +1.1 2,181,883 - thiD b2103 0.18 -0.4 2,181,883 - thiD b2103 0.18 +0.5 2,182,009 + thiD b2103 0.34 -0.5 2,182,009 + thiD b2103 0.34 +0.4 2,182,012 + thiD b2103 0.34 -0.4 2,182,020 - thiD b2103 0.35 -1.0 2,182,119 - thiD b2103 0.48 +0.4 2,182,150 - thiD b2103 0.51 -0.8 2,182,161 + thiD b2103 0.53 +0.1 2,182,375 + thiD b2103 0.80 -0.4 2,182,375 + thiD b2103 0.80 +1.7 2,182,382 + thiD b2103 0.80 +0.5 2,182,546 - -0.1 2,182,646 + thiM b2104 0.14 -0.6 2,183,006 - thiM b2104 0.60 -0.2 2,183,006 - thiM b2104 0.60 -0.9 2,183,044 + thiM b2104 0.65 -0.4 2,183,246 + +0.1 2,183,246 + -0.6 2,183,259 - +1.8 2,183,259 - -0.7 2,183,389 - -1.0 2,183,399 - -0.0 2,183,399 - -0.4 2,183,399 - +0.0 2,183,495 - -1.0 2,183,533 - -0.1 2,183,614 + yohL b2105 0.25 +0.6 2,183,614 - yohL b2105 0.25 -0.1 2,183,620 - yohL b2105 0.27 -0.6 2,183,621 + yohL b2105 0.27 -0.9 2,183,766 - yohL b2105 0.81 -0.0 2,183,814 - -1.8 2,183,814 - +2.4 2,183,989 + +0.1 2,184,011 + -0.5 2,184,014 + -0.2 2,184,014 + -0.3 2,184,030 - yohM b2106 0.11 -1.6 2,184,141 + yohM b2106 0.24 +0.8 2,184,141 + yohM b2106 0.24 +0.7 2,184,272 + yohM b2106 0.40 +0.4
Or see this region's nucleotide sequence