Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydfA and ydfB are separated by 1 nucleotides ydfB and ydfC are separated by 29 nucleotides ydfC and dicF are separated by 340 nucleotides dicF and dicB are separated by 174 nucleotides dicB and ydfD overlap by 4 nucleotides ydfD and ydfE are separated by 92 nucleotides
b1571: ydfA - Qin prophage; predicted protein (NCBI), at 1,646,532 to 1,646,687
ydfA
b1572: ydfB - orf, hypothetical protein (VIMSS), at 1,646,689 to 1,646,817
ydfB
b1573: ydfC - Qin prophage; predicted protein (RefSeq), at 1,646,847 to 1,647,065
ydfC
b1574: dicF - antisense RNA DicF (NCBI), at 1,647,406 to 1,647,458
dicF
b1575: dicB - Qin prophage; cell division inhibition protein (NCBI), at 1,647,633 to 1,647,821
dicB
b1576: ydfD - Qin prophage; predicted protein (NCBI), at 1,647,818 to 1,648,009
ydfD
b1577: ydfE - Qin prophage; predicted protein (RefSeq), at 1,648,102 to 1,648,866
ydfE
Position (kb)
1647
1648 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1646.751 kb on - strand, within ydfB at 1646.887 kb on - strand, within ydfC at 1646.997 kb on - strand, within ydfC at 1647.366 kb on - strand at 1647.500 kb on - strand at 1647.653 kb on + strand, within dicB at 1647.700 kb on + strand, within dicB at 1647.709 kb on + strand, within dicB at 1647.775 kb on - strand, within dicB at 1647.880 kb on - strand, within ydfD at 1647.900 kb on - strand, within ydfD at 1648.158 kb on - strand at 1648.158 kb on - strand at 1648.173 kb on + strand at 1648.173 kb on + strand at 1648.176 kb on - strand at 1648.176 kb on - strand at 1648.191 kb on - strand, within ydfE at 1648.250 kb on - strand, within ydfE at 1648.252 kb on + strand, within ydfE at 1648.260 kb on - strand, within ydfE at 1648.260 kb on - strand, within ydfE at 1648.260 kb on - strand, within ydfE at 1648.260 kb on - strand, within ydfE at 1648.297 kb on + strand, within ydfE at 1648.301 kb on - strand, within ydfE at 1648.301 kb on - strand, within ydfE at 1648.305 kb on - strand, within ydfE at 1648.306 kb on + strand, within ydfE at 1648.333 kb on + strand, within ydfE at 1648.357 kb on + strand, within ydfE at 1648.391 kb on - strand, within ydfE at 1648.391 kb on - strand, within ydfE at 1648.446 kb on - strand, within ydfE at 1648.725 kb on + strand, within ydfE
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,646,751 - ydfB b1572 0.48 +0.5 1,646,887 - ydfC b1573 0.18 +0.8 1,646,997 - ydfC b1573 0.68 +1.0 1,647,366 - +0.7 1,647,500 - +0.7 1,647,653 + dicB b1575 0.11 +0.1 1,647,700 + dicB b1575 0.35 +0.3 1,647,709 + dicB b1575 0.40 -1.8 1,647,775 - dicB b1575 0.75 +0.7 1,647,880 - ydfD b1576 0.32 +0.7 1,647,900 - ydfD b1576 0.43 +0.7 1,648,158 - +0.1 1,648,158 - +2.3 1,648,173 + +0.7 1,648,173 + -1.3 1,648,176 - +0.3 1,648,176 - +1.0 1,648,191 - ydfE b1577 0.12 +0.1 1,648,250 - ydfE b1577 0.19 +1.3 1,648,252 + ydfE b1577 0.20 +1.4 1,648,260 - ydfE b1577 0.21 +0.2 1,648,260 - ydfE b1577 0.21 +0.3 1,648,260 - ydfE b1577 0.21 +1.3 1,648,260 - ydfE b1577 0.21 -0.1 1,648,297 + ydfE b1577 0.25 +0.1 1,648,301 - ydfE b1577 0.26 -0.6 1,648,301 - ydfE b1577 0.26 +0.3 1,648,305 - ydfE b1577 0.27 -0.0 1,648,306 + ydfE b1577 0.27 -0.1 1,648,333 + ydfE b1577 0.30 +0.7 1,648,357 + ydfE b1577 0.33 +0.7 1,648,391 - ydfE b1577 0.38 -1.3 1,648,391 - ydfE b1577 0.38 +0.1 1,648,446 - ydfE b1577 0.45 -0.5 1,648,725 + ydfE b1577 0.81 +0.5
Or see this region's nucleotide sequence