Strain Fitness in Escherichia coli BW25113 around b3195

Experiment: Nalidixic 0.0313 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nalidixic 0.0313 mM
3,336,286	+	yrbD	b3193	0.64	-2.6
3,336,294	-	yrbD	b3193	0.66	-3.4
3,336,294	-	yrbD	b3193	0.66	-2.7
3,336,294	-	yrbD	b3193	0.66	-3.4
3,336,308	-	yrbD	b3193	0.68	+0.6
3,336,332	+	yrbD	b3193	0.72	-1.4
3,336,340	-	yrbD	b3193	0.74	-3.1
3,336,362	+	yrbD	b3193	0.78	-3.5
3,336,362	-	yrbD	b3193	0.78	-1.4
3,336,367	+	yrbD	b3193	0.79	-1.4
3,336,377	+	yrbD	b3193	0.81	-2.3
3,336,380	-	yrbD	b3193	0.81	-2.9
3,336,380	-	yrbD	b3193	0.81	-1.1
3,336,385	-	yrbD	b3193	0.82	-1.6
3,336,387	-	yrbD	b3193	0.82	-2.2
3,336,402	+	yrbD	b3193	0.85	-2.8
3,336,440	-				-3.8
3,336,440	-				-2.9
3,336,445	+				-1.0
3,336,445	+				-2.0
3,336,453	-				-0.9
3,336,453	-				-1.4
3,336,526	+				-2.1
3,336,619	+	yrbE	b3194	0.17	-2.1
3,336,619	-	yrbE	b3194	0.17	-2.3
3,336,636	+	yrbE	b3194	0.19	-0.3
3,336,636	+	yrbE	b3194	0.19	-1.6
3,336,666	-				-0.6
3,336,669	-	yrbE	b3194	0.23	-1.0
3,336,678	+	yrbE	b3194	0.24	-3.1
3,336,678	+	yrbE	b3194	0.24	-3.0
3,336,711	+	yrbE	b3194	0.28	-1.4
3,336,711	+	yrbE	b3194	0.28	-1.6
3,336,736	+	yrbE	b3194	0.32	-2.5
3,336,773	-	yrbE	b3194	0.36	-2.4
3,336,800	-	yrbE	b3194	0.40	-2.5
3,336,804	+	yrbE	b3194	0.40	-2.5
3,336,810	+	yrbE	b3194	0.41	+1.0
3,336,822	+	yrbE	b3194	0.43	-2.0
3,336,827	+	yrbE	b3194	0.43	-1.2
3,336,853	+	yrbE	b3194	0.47	-1.8
3,336,879	+	yrbE	b3194	0.50	-1.8
3,336,879	+	yrbE	b3194	0.50	-1.8
3,336,887	-	yrbE	b3194	0.51	-3.1
3,336,894	+	yrbE	b3194	0.52	+1.0
3,336,894	+	yrbE	b3194	0.52	-0.3
3,336,902	+	yrbE	b3194	0.53	-2.4
3,336,902	+	yrbE	b3194	0.53	-2.1
3,336,910	-	yrbE	b3194	0.54	-3.4
3,336,910	-	yrbE	b3194	0.54	-2.4
3,336,926	+	yrbE	b3194	0.56	-2.4
3,336,951	-	yrbE	b3194	0.59	-2.1
3,336,951	-	yrbE	b3194	0.59	-2.6
3,337,024	+	yrbE	b3194	0.68	-1.9
3,337,024	+	yrbE	b3194	0.68	-1.4
3,337,047	-	yrbE	b3194	0.71	-2.4
3,337,047	-	yrbE	b3194	0.71	-2.8
3,337,050	+	yrbE	b3194	0.72	-2.9
3,337,057	+	yrbE	b3194	0.73	-3.1
3,337,057	+	yrbE	b3194	0.73	-3.1
3,337,066	-	yrbE	b3194	0.74	-1.5
3,337,066	-	yrbE	b3194	0.74	-2.4
3,337,067	-	yrbE	b3194	0.74	-2.6
3,337,119	+	yrbE	b3194	0.81	-1.6
3,337,141	+	yrbE	b3194	0.83	-2.3
3,337,153	-	yrbE	b3194	0.85	-1.4
3,337,170	+	yrbE	b3194	0.87	-3.5
3,337,187	+	yrbE	b3194	0.89	-2.7
3,337,200	-				-2.4
3,337,257	+				-1.3
3,337,265	+				-1.8
3,337,265	+				-2.2
3,337,271	+				-2.8
3,337,281	+				-1.8
3,337,294	+				-2.2
3,337,328	+				-3.1
3,337,331	+				-1.5
3,337,331	+				-1.4
3,337,334	-				-3.5
3,337,334	-				-2.1
3,337,334	-				-1.8
3,337,343	+				-2.6
3,337,351	-				-2.7
3,337,351	-				-2.0
3,337,397	+	yrbF	b3195	0.15	-2.2
3,337,398	-	yrbF	b3195	0.15	-1.5
3,337,402	+	yrbF	b3195	0.15	-1.4
3,337,448	+	yrbF	b3195	0.21	-3.3
3,337,448	+	yrbF	b3195	0.21	-0.6
3,337,458	+	yrbF	b3195	0.22	-0.8
3,337,475	-	yrbF	b3195	0.24	-2.6
3,337,505	-	yrbF	b3195	0.28	-4.1
3,337,518	-	yrbF	b3195	0.30	-2.1
3,337,663	-	yrbF	b3195	0.48	-3.7
3,337,663	-	yrbF	b3195	0.48	-1.8
3,337,677	-	yrbF	b3195	0.49	-2.4
3,337,677	-	yrbF	b3195	0.49	-1.8
3,337,677	-	yrbF	b3195	0.49	-3.0
3,337,686	-	yrbF	b3195	0.50	-2.5
3,337,698	+	yrbF	b3195	0.52	-1.4
3,337,698	+	yrbF	b3195	0.52	-2.7
3,337,704	+	yrbF	b3195	0.53	-1.7
3,337,742	+	yrbF	b3195	0.57	-3.4
3,337,750	-	yrbF	b3195	0.58	-2.6
3,337,751	+	yrbF	b3195	0.58	-1.6
3,337,762	+	yrbF	b3195	0.60	-2.0
3,337,784	-	yrbF	b3195	0.62	-3.8
3,337,794	+	yrbF	b3195	0.64	-2.4
3,337,795	+	yrbF	b3195	0.64	-1.0
3,337,795	+	yrbF	b3195	0.64	-2.8
3,337,841	+	yrbF	b3195	0.70	-1.5
3,337,841	+	yrbF	b3195	0.70	-1.7
3,337,843	+	yrbF	b3195	0.70	-2.1
3,337,843	+	yrbF	b3195	0.70	-3.0
3,337,849	-	yrbF	b3195	0.70	-4.1
3,337,849	-	yrbF	b3195	0.70	-5.3
3,337,849	-	yrbF	b3195	0.70	-2.0
3,337,851	-	yrbF	b3195	0.71	-2.9
3,337,851	-	yrbF	b3195	0.71	-3.8
3,337,851	-	yrbF	b3195	0.71	-2.3
3,337,851	-	yrbF	b3195	0.71	-3.7
3,337,851	-	yrbF	b3195	0.71	-3.0
3,337,851	-	yrbF	b3195	0.71	-2.8
3,337,851	-	yrbF	b3195	0.71	-3.7
3,337,893	-	yrbF	b3195	0.76	-2.5
3,337,899	+	yrbF	b3195	0.77	-2.3
3,337,906	-	yrbF	b3195	0.78	-0.8
3,337,906	-	yrbF	b3195	0.78	-2.2
3,338,003	+	yrbF	b3195	0.90	-1.6
3,338,084	+				-4.2
3,338,113	+				-1.5
3,338,132	+				-1.7
3,338,145	-				+0.3
3,338,170	-				-0.7
3,338,254	+				-0.5
3,338,278	+				-2.4
3,338,300	-				-0.0
3,338,357	-				-0.1
3,338,357	-				-0.2
3,338,436	-	yrbG	b3196	0.14	-0.5
3,338,436	-	yrbG	b3196	0.14	+0.6
3,338,436	-	yrbG	b3196	0.14	+0.0
3,338,436	-	yrbG	b3196	0.14	-0.1
3,338,462	+	yrbG	b3196	0.17	+0.2
3,338,503	+	yrbG	b3196	0.21	-0.0
3,338,508	+	yrbG	b3196	0.22	-0.7
3,338,508	+	yrbG	b3196	0.22	+0.1
3,338,553	+	yrbG	b3196	0.26	+0.4
3,338,591	+	yrbG	b3196	0.30	+0.3
3,338,594	+	yrbG	b3196	0.30	+0.1
3,338,601	+	yrbG	b3196	0.31	+1.1
3,338,612	+	yrbG	b3196	0.32	-1.5
3,338,612	+	yrbG	b3196	0.32	+1.5
3,338,626	+	yrbG	b3196	0.34	-2.1
3,338,629	-	yrbG	b3196	0.34	-1.1
3,338,629	-	yrbG	b3196	0.34	+0.2
3,338,643	+	yrbG	b3196	0.35	-0.0
3,338,646	-	yrbG	b3196	0.36	+0.1
3,338,651	-	yrbG	b3196	0.36	+0.1
3,338,693	-				-1.4
3,338,714	-	yrbG	b3196	0.43	+0.3
3,338,714	-	yrbG	b3196	0.43	-0.4
3,338,714	-	yrbG	b3196	0.43	+0.1
3,338,722	-	yrbG	b3196	0.43	-1.1
3,338,768	-	yrbG	b3196	0.48	-0.3
3,338,789	+	yrbG	b3196	0.50	-0.8
3,338,792	-	yrbG	b3196	0.51	-1.5
3,338,797	-	yrbG	b3196	0.51	+0.2
3,338,797	-	yrbG	b3196	0.51	-0.6
3,338,876	+	yrbG	b3196	0.59	-0.5
3,338,891	+	yrbG	b3196	0.61	-0.7
3,338,897	+	yrbG	b3196	0.61	-0.2
3,338,960	-	yrbG	b3196	0.68	-1.6
3,338,984	-	yrbG	b3196	0.70	-1.6
3,339,031	-	yrbG	b3196	0.75	-1.4