Experiment: Nalidixic 0.0313 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yqiB and nudF are separated by 0 nucleotides nudF and tolC are separated by 204 nucleotides tolC and ygiB are separated by 147 nucleotides ygiB and ygiC are separated by 5 nucleotides
b3033: yqiB - predicted dehydrogenase (NCBI), at 3,174,880 to 3,175,302
yqiB
b3034: nudF - ADP-ribose pyrophosphatase (NCBI), at 3,175,303 to 3,175,932
nudF
b3035: tolC - outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS), at 3,176,137 to 3,177,618
tolC
b3037: ygiB - conserved outer membrane protein (RefSeq), at 3,177,766 to 3,178,437
ygiB
b3038: ygiC - predicted enzyme (NCBI), at 3,178,443 to 3,179,603
ygiC
Position (kb)
3176
3177
3178 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3175.171 kb on - strand, within yqiB at 3175.226 kb on + strand, within yqiB at 3175.399 kb on - strand, within nudF at 3175.469 kb on - strand, within nudF at 3175.494 kb on - strand, within nudF at 3175.494 kb on - strand, within nudF at 3175.528 kb on - strand, within nudF at 3175.577 kb on + strand, within nudF at 3175.585 kb on + strand, within nudF at 3175.590 kb on - strand, within nudF at 3175.662 kb on + strand, within nudF at 3175.679 kb on + strand, within nudF at 3175.679 kb on + strand, within nudF at 3175.752 kb on - strand, within nudF at 3175.798 kb on + strand, within nudF at 3175.818 kb on - strand, within nudF at 3175.823 kb on - strand, within nudF at 3175.871 kb on - strand at 3176.030 kb on - strand at 3176.031 kb on + strand at 3176.119 kb on - strand at 3176.119 kb on - strand at 3176.173 kb on - strand at 3176.173 kb on - strand at 3176.174 kb on + strand at 3176.182 kb on - strand at 3176.208 kb on - strand at 3176.271 kb on + strand at 3176.271 kb on + strand at 3176.304 kb on + strand, within tolC at 3176.309 kb on + strand, within tolC at 3176.309 kb on + strand, within tolC at 3176.349 kb on - strand, within tolC at 3176.378 kb on + strand, within tolC at 3176.493 kb on + strand, within tolC at 3176.526 kb on - strand, within tolC at 3176.571 kb on + strand, within tolC at 3176.573 kb on + strand, within tolC at 3176.581 kb on - strand, within tolC at 3176.635 kb on + strand, within tolC at 3176.638 kb on - strand, within tolC at 3176.654 kb on - strand, within tolC at 3176.680 kb on - strand, within tolC at 3176.680 kb on - strand, within tolC at 3176.682 kb on + strand, within tolC at 3176.692 kb on - strand, within tolC at 3176.716 kb on + strand, within tolC at 3176.716 kb on + strand, within tolC at 3176.844 kb on - strand, within tolC at 3176.844 kb on - strand, within tolC at 3176.869 kb on - strand, within tolC at 3176.869 kb on - strand, within tolC at 3176.887 kb on + strand, within tolC at 3176.887 kb on + strand, within tolC at 3176.887 kb on + strand, within tolC at 3176.932 kb on + strand, within tolC at 3176.934 kb on + strand, within tolC at 3176.942 kb on - strand, within tolC at 3176.979 kb on - strand, within tolC at 3176.979 kb on - strand, within tolC at 3176.981 kb on + strand, within tolC at 3176.981 kb on + strand, within tolC at 3176.988 kb on - strand, within tolC at 3177.093 kb on + strand, within tolC at 3177.105 kb on + strand, within tolC at 3177.113 kb on - strand, within tolC at 3177.120 kb on + strand, within tolC at 3177.339 kb on + strand, within tolC at 3177.339 kb on + strand, within tolC at 3177.437 kb on + strand, within tolC at 3177.488 kb on + strand at 3177.516 kb on - strand at 3177.517 kb on + strand at 3177.517 kb on + strand at 3177.717 kb on + strand at 3177.737 kb on + strand at 3177.747 kb on - strand at 3177.964 kb on + strand, within ygiB at 3177.964 kb on + strand, within ygiB at 3177.964 kb on + strand, within ygiB at 3177.964 kb on - strand, within ygiB at 3178.163 kb on + strand, within ygiB at 3178.238 kb on + strand, within ygiB at 3178.238 kb on + strand, within ygiB at 3178.276 kb on + strand, within ygiB at 3178.276 kb on + strand, within ygiB at 3178.294 kb on - strand, within ygiB at 3178.298 kb on - strand, within ygiB at 3178.298 kb on - strand, within ygiB at 3178.390 kb on + strand at 3178.390 kb on + strand at 3178.398 kb on - strand at 3178.423 kb on - strand at 3178.454 kb on + strand at 3178.540 kb on - strand at 3178.542 kb on + strand at 3178.545 kb on - strand at 3178.546 kb on - strand at 3178.546 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.0313 mM remove 3,175,171 - yqiB b3033 0.69 +0.4 3,175,226 + yqiB b3033 0.82 +0.8 3,175,399 - nudF b3034 0.15 +0.5 3,175,469 - nudF b3034 0.26 -0.3 3,175,494 - nudF b3034 0.30 +0.5 3,175,494 - nudF b3034 0.30 +0.7 3,175,528 - nudF b3034 0.36 +1.0 3,175,577 + nudF b3034 0.43 +0.7 3,175,585 + nudF b3034 0.45 +0.1 3,175,590 - nudF b3034 0.46 +1.0 3,175,662 + nudF b3034 0.57 -0.3 3,175,679 + nudF b3034 0.60 -0.2 3,175,679 + nudF b3034 0.60 -0.2 3,175,752 - nudF b3034 0.71 +0.3 3,175,798 + nudF b3034 0.79 -0.8 3,175,818 - nudF b3034 0.82 -0.1 3,175,823 - nudF b3034 0.83 -0.7 3,175,871 - +0.2 3,176,030 - -0.3 3,176,031 + -0.2 3,176,119 - -2.6 3,176,119 - -0.7 3,176,173 - -3.9 3,176,173 - -1.2 3,176,174 + -3.2 3,176,182 - +1.0 3,176,208 - -2.0 3,176,271 + -4.2 3,176,271 + -4.3 3,176,304 + tolC b3035 0.11 -2.9 3,176,309 + tolC b3035 0.12 -3.3 3,176,309 + tolC b3035 0.12 -1.6 3,176,349 - tolC b3035 0.14 -1.0 3,176,378 + tolC b3035 0.16 -4.2 3,176,493 + tolC b3035 0.24 -2.9 3,176,526 - tolC b3035 0.26 -3.9 3,176,571 + tolC b3035 0.29 -1.3 3,176,573 + tolC b3035 0.29 -2.2 3,176,581 - tolC b3035 0.30 -3.1 3,176,635 + tolC b3035 0.34 -3.5 3,176,638 - tolC b3035 0.34 -2.6 3,176,654 - tolC b3035 0.35 -3.3 3,176,680 - tolC b3035 0.37 -1.8 3,176,680 - tolC b3035 0.37 -1.3 3,176,682 + tolC b3035 0.37 -2.8 3,176,692 - tolC b3035 0.37 -5.4 3,176,716 + tolC b3035 0.39 -2.2 3,176,716 + tolC b3035 0.39 -3.1 3,176,844 - tolC b3035 0.48 -0.6 3,176,844 - tolC b3035 0.48 -2.2 3,176,869 - tolC b3035 0.49 -2.8 3,176,869 - tolC b3035 0.49 -1.3 3,176,887 + tolC b3035 0.51 -2.7 3,176,887 + tolC b3035 0.51 -3.5 3,176,887 + tolC b3035 0.51 -5.1 3,176,932 + tolC b3035 0.54 -2.6 3,176,934 + tolC b3035 0.54 -4.0 3,176,942 - tolC b3035 0.54 -4.4 3,176,979 - tolC b3035 0.57 -0.6 3,176,979 - tolC b3035 0.57 -0.0 3,176,981 + tolC b3035 0.57 -4.4 3,176,981 + tolC b3035 0.57 -0.0 3,176,988 - tolC b3035 0.57 -2.0 3,177,093 + tolC b3035 0.65 -2.9 3,177,105 + tolC b3035 0.65 -3.1 3,177,113 - tolC b3035 0.66 -2.3 3,177,120 + tolC b3035 0.66 -3.0 3,177,339 + tolC b3035 0.81 -2.2 3,177,339 + tolC b3035 0.81 -1.6 3,177,437 + tolC b3035 0.88 -4.4 3,177,488 + +0.2 3,177,516 - -2.5 3,177,517 + +0.6 3,177,517 + +0.4 3,177,717 + +0.1 3,177,737 + -0.3 3,177,747 - -0.1 3,177,964 + ygiB b3037 0.29 +0.6 3,177,964 + ygiB b3037 0.29 -0.1 3,177,964 + ygiB b3037 0.29 -1.2 3,177,964 - ygiB b3037 0.29 -0.4 3,178,163 + ygiB b3037 0.59 -0.2 3,178,238 + ygiB b3037 0.70 -0.4 3,178,238 + ygiB b3037 0.70 -0.8 3,178,276 + ygiB b3037 0.76 +1.6 3,178,276 + ygiB b3037 0.76 +0.1 3,178,294 - ygiB b3037 0.79 +0.1 3,178,298 - ygiB b3037 0.79 -0.1 3,178,298 - ygiB b3037 0.79 -0.6 3,178,390 + -1.2 3,178,390 + -0.9 3,178,398 - -0.5 3,178,423 - -0.1 3,178,454 + -0.6 3,178,540 - +0.0 3,178,542 + -1.0 3,178,545 - -0.5 3,178,546 - +0.5 3,178,546 - -0.8
Or see this region's nucleotide sequence