Experiment: Nalidixic 0.0313 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt htpX and prc are separated by 191 nucleotides prc and proQ are separated by 19 nucleotides proQ and yebR are separated by 96 nucleotides
b1829: htpX - heat shock protein HtpX (NCBI), at 1,909,719 to 1,910,600
htpX
b1830: prc - carboxy-terminal protease for penicillin-binding protein 3 (NCBI), at 1,910,792 to 1,912,840
prc
b1831: proQ - putative solute/DNA competence effector (NCBI), at 1,912,860 to 1,913,558
proQ
b1832: yebR - free methionine-(R)-sulfoxide reductase (RefSeq), at 1,913,655 to 1,914,152
yebR
Position (kb)
1910
1911
1912
1913 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1909.831 kb on - strand, within htpX at 1909.831 kb on - strand, within htpX at 1910.032 kb on + strand, within htpX at 1910.119 kb on + strand, within htpX at 1910.119 kb on + strand, within htpX at 1910.121 kb on + strand, within htpX at 1910.143 kb on - strand, within htpX at 1910.228 kb on - strand, within htpX at 1910.486 kb on - strand, within htpX at 1910.486 kb on - strand, within htpX at 1910.624 kb on + strand at 1910.643 kb on + strand at 1910.686 kb on + strand at 1910.686 kb on + strand at 1910.877 kb on - strand at 1910.898 kb on - strand at 1911.010 kb on - strand at 1911.068 kb on + strand, within prc at 1911.197 kb on - strand, within prc at 1911.283 kb on + strand, within prc at 1911.318 kb on + strand, within prc at 1911.341 kb on - strand, within prc at 1911.341 kb on - strand, within prc at 1911.400 kb on - strand, within prc at 1911.400 kb on - strand, within prc at 1911.511 kb on - strand, within prc at 1911.511 kb on - strand, within prc at 1911.530 kb on - strand, within prc at 1911.546 kb on - strand, within prc at 1911.546 kb on - strand, within prc at 1911.606 kb on + strand, within prc at 1911.651 kb on + strand, within prc at 1911.749 kb on - strand, within prc at 1911.749 kb on - strand, within prc at 1911.847 kb on + strand, within prc at 1911.847 kb on + strand, within prc at 1912.082 kb on + strand, within prc at 1912.123 kb on - strand, within prc at 1912.140 kb on + strand, within prc at 1912.140 kb on + strand, within prc at 1912.166 kb on + strand, within prc at 1912.166 kb on + strand, within prc at 1912.759 kb on + strand at 1912.759 kb on + strand at 1912.794 kb on - strand at 1912.972 kb on + strand, within proQ at 1912.972 kb on + strand, within proQ at 1913.231 kb on - strand, within proQ at 1913.263 kb on - strand, within proQ at 1913.263 kb on - strand, within proQ at 1913.405 kb on + strand, within proQ at 1913.405 kb on + strand, within proQ at 1913.413 kb on - strand, within proQ at 1913.725 kb on - strand, within yebR at 1913.752 kb on + strand, within yebR at 1913.752 kb on + strand, within yebR at 1913.760 kb on - strand, within yebR at 1913.790 kb on - strand, within yebR at 1913.790 kb on - strand, within yebR
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.0313 mM remove 1,909,831 - htpX b1829 0.13 +0.5 1,909,831 - htpX b1829 0.13 -0.2 1,910,032 + htpX b1829 0.35 -1.4 1,910,119 + htpX b1829 0.45 -0.6 1,910,119 + htpX b1829 0.45 +0.2 1,910,121 + htpX b1829 0.46 -0.8 1,910,143 - htpX b1829 0.48 +0.0 1,910,228 - htpX b1829 0.58 -0.8 1,910,486 - htpX b1829 0.87 +0.8 1,910,486 - htpX b1829 0.87 -0.5 1,910,624 + -0.3 1,910,643 + -1.1 1,910,686 + +0.2 1,910,686 + -0.2 1,910,877 - -2.9 1,910,898 - -2.4 1,911,010 - -0.6 1,911,068 + prc b1830 0.13 -1.6 1,911,197 - prc b1830 0.20 -2.5 1,911,283 + prc b1830 0.24 -1.6 1,911,318 + prc b1830 0.26 -2.5 1,911,341 - prc b1830 0.27 -1.4 1,911,341 - prc b1830 0.27 -0.0 1,911,400 - prc b1830 0.30 -1.8 1,911,400 - prc b1830 0.30 -0.6 1,911,511 - prc b1830 0.35 -2.2 1,911,511 - prc b1830 0.35 -2.0 1,911,530 - prc b1830 0.36 -2.5 1,911,546 - prc b1830 0.37 -2.0 1,911,546 - prc b1830 0.37 -0.6 1,911,606 + prc b1830 0.40 -1.4 1,911,651 + prc b1830 0.42 -2.9 1,911,749 - prc b1830 0.47 -1.6 1,911,749 - prc b1830 0.47 -3.0 1,911,847 + prc b1830 0.51 -1.8 1,911,847 + prc b1830 0.51 -1.4 1,912,082 + prc b1830 0.63 -3.9 1,912,123 - prc b1830 0.65 -1.4 1,912,140 + prc b1830 0.66 -1.0 1,912,140 + prc b1830 0.66 -0.0 1,912,166 + prc b1830 0.67 -2.7 1,912,166 + prc b1830 0.67 -3.4 1,912,759 + -1.0 1,912,759 + -3.6 1,912,794 - -3.0 1,912,972 + proQ b1831 0.16 -2.3 1,912,972 + proQ b1831 0.16 -2.4 1,913,231 - proQ b1831 0.53 -2.5 1,913,263 - proQ b1831 0.58 -0.4 1,913,263 - proQ b1831 0.58 -0.0 1,913,405 + proQ b1831 0.78 -1.0 1,913,405 + proQ b1831 0.78 -1.0 1,913,413 - proQ b1831 0.79 +0.2 1,913,725 - yebR b1832 0.14 +0.3 1,913,752 + yebR b1832 0.19 +0.4 1,913,752 + yebR b1832 0.19 -0.6 1,913,760 - yebR b1832 0.21 -0.6 1,913,790 - yebR b1832 0.27 +0.3 1,913,790 - yebR b1832 0.27 -0.1
Or see this region's nucleotide sequence