Experiment: Nalidixic 0.0313 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uidA and uidR are separated by 390 nucleotides uidR and hdhA are separated by 220 nucleotides
b1617: uidA - beta-D-glucuronidase (NCBI), at 1,692,284 to 1,694,095
uidA
b1618: uidR - DNA-binding transcriptional repressor (NCBI), at 1,694,486 to 1,695,076
uidR
b1619: hdhA - 7-alpha-hydroxysteroid dehydrogenase (NCBI), at 1,695,297 to 1,696,064
hdhA
Position (kb)
1694
1695
1696 Strain fitness (log2 ratio)
-1
0
1
2 at 1693.548 kb on - strand, within uidA at 1693.548 kb on - strand, within uidA at 1693.571 kb on - strand, within uidA at 1693.571 kb on - strand, within uidA at 1693.836 kb on - strand, within uidA at 1693.954 kb on + strand at 1694.042 kb on - strand at 1694.100 kb on - strand at 1694.177 kb on - strand at 1694.267 kb on - strand at 1694.629 kb on + strand at 1694.629 kb on + strand, within uidR at 1694.746 kb on - strand, within uidR at 1694.873 kb on + strand, within uidR at 1694.873 kb on + strand, within uidR at 1694.938 kb on + strand, within uidR at 1695.083 kb on + strand at 1695.089 kb on - strand at 1695.234 kb on - strand at 1695.316 kb on - strand at 1695.368 kb on + strand at 1695.368 kb on + strand at 1695.393 kb on - strand, within hdhA at 1695.425 kb on + strand, within hdhA at 1695.432 kb on - strand, within hdhA at 1695.432 kb on - strand, within hdhA at 1695.558 kb on - strand, within hdhA at 1695.574 kb on + strand, within hdhA at 1695.574 kb on + strand, within hdhA at 1695.574 kb on + strand, within hdhA at 1695.582 kb on - strand, within hdhA at 1695.582 kb on - strand, within hdhA at 1695.592 kb on - strand, within hdhA at 1695.592 kb on - strand, within hdhA at 1695.690 kb on - strand, within hdhA at 1695.690 kb on - strand, within hdhA at 1695.724 kb on - strand, within hdhA at 1695.758 kb on - strand, within hdhA at 1695.771 kb on - strand, within hdhA at 1695.771 kb on - strand, within hdhA at 1695.779 kb on - strand, within hdhA at 1695.779 kb on - strand, within hdhA at 1695.841 kb on - strand, within hdhA at 1695.841 kb on - strand, within hdhA at 1695.901 kb on - strand, within hdhA at 1695.914 kb on - strand, within hdhA at 1695.925 kb on - strand, within hdhA at 1695.959 kb on + strand, within hdhA at 1695.959 kb on + strand, within hdhA at 1696.001 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.0313 mM remove 1,693,548 - uidA b1617 0.70 -0.4 1,693,548 - uidA b1617 0.70 -0.1 1,693,571 - uidA b1617 0.71 -0.5 1,693,571 - uidA b1617 0.71 +0.2 1,693,836 - uidA b1617 0.86 +0.0 1,693,954 + +0.3 1,694,042 - -0.1 1,694,100 - +0.4 1,694,177 - -0.8 1,694,267 - -0.3 1,694,629 + +0.6 1,694,629 + uidR b1618 0.24 -0.0 1,694,746 - uidR b1618 0.44 -0.4 1,694,873 + uidR b1618 0.65 -0.8 1,694,873 + uidR b1618 0.65 -1.7 1,694,938 + uidR b1618 0.76 -0.4 1,695,083 + +0.6 1,695,089 - -0.8 1,695,234 - -0.6 1,695,316 - +0.8 1,695,368 + -0.7 1,695,368 + -1.6 1,695,393 - hdhA b1619 0.12 -0.8 1,695,425 + hdhA b1619 0.17 -0.1 1,695,432 - hdhA b1619 0.18 -0.5 1,695,432 - hdhA b1619 0.18 +0.5 1,695,558 - hdhA b1619 0.34 -0.0 1,695,574 + hdhA b1619 0.36 -1.3 1,695,574 + hdhA b1619 0.36 -0.3 1,695,574 + hdhA b1619 0.36 +1.0 1,695,582 - hdhA b1619 0.37 +2.3 1,695,582 - hdhA b1619 0.37 -1.1 1,695,592 - hdhA b1619 0.38 +0.5 1,695,592 - hdhA b1619 0.38 -0.4 1,695,690 - hdhA b1619 0.51 +0.1 1,695,690 - hdhA b1619 0.51 -0.1 1,695,724 - hdhA b1619 0.56 -1.0 1,695,758 - hdhA b1619 0.60 -0.6 1,695,771 - hdhA b1619 0.62 -0.4 1,695,771 - hdhA b1619 0.62 +0.8 1,695,779 - hdhA b1619 0.63 -1.1 1,695,779 - hdhA b1619 0.63 +0.0 1,695,841 - hdhA b1619 0.71 -1.0 1,695,841 - hdhA b1619 0.71 +0.3 1,695,901 - hdhA b1619 0.79 -0.5 1,695,914 - hdhA b1619 0.80 -1.1 1,695,925 - hdhA b1619 0.82 +0.6 1,695,959 + hdhA b1619 0.86 +1.0 1,695,959 + hdhA b1619 0.86 -0.3 1,696,001 - -0.4
Or see this region's nucleotide sequence