Experiment: Nalidixic 0.0313 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybjD and ybjX overlap by 4 nucleotides ybjX and b0878 are separated by 114 nucleotides
b0876: ybjD - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 915,696 to 917,354
ybjD
b0877: ybjX - hypothetical protein (NCBI), at 917,351 to 918,343
ybjX
b0878: b0878 - putative membrane protein (VIMSS), at 918,458 to 919,573
b0878
Position (kb)
917
918
919 Strain fitness (log2 ratio)
-2
-1
0
1 at 916.374 kb on - strand, within ybjD at 916.463 kb on + strand, within ybjD at 916.463 kb on + strand, within ybjD at 916.540 kb on - strand, within ybjD at 916.546 kb on + strand, within ybjD at 916.717 kb on - strand, within ybjD at 916.717 kb on - strand, within ybjD at 916.823 kb on + strand, within ybjD at 916.823 kb on + strand, within ybjD at 916.869 kb on + strand, within ybjD at 916.869 kb on + strand, within ybjD at 916.989 kb on + strand, within ybjD at 917.422 kb on - strand at 917.467 kb on + strand, within ybjX at 917.467 kb on + strand, within ybjX at 917.528 kb on + strand, within ybjX at 917.528 kb on + strand, within ybjX at 917.643 kb on - strand, within ybjX at 917.658 kb on - strand, within ybjX at 917.658 kb on - strand, within ybjX at 917.775 kb on + strand, within ybjX at 917.828 kb on + strand, within ybjX at 917.838 kb on + strand, within ybjX at 917.878 kb on + strand, within ybjX at 917.878 kb on + strand, within ybjX at 917.891 kb on - strand, within ybjX at 917.948 kb on - strand, within ybjX at 918.050 kb on - strand, within ybjX at 918.059 kb on + strand, within ybjX at 918.271 kb on + strand at 918.484 kb on + strand at 918.620 kb on + strand, within b0878 at 918.789 kb on - strand, within b0878 at 918.849 kb on - strand at 918.849 kb on - strand, within b0878 at 918.985 kb on + strand, within b0878 at 919.227 kb on - strand, within b0878 at 919.262 kb on + strand, within b0878 at 919.262 kb on + strand, within b0878 at 919.298 kb on - strand, within b0878 at 919.298 kb on - strand, within b0878 at 919.343 kb on + strand, within b0878
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.0313 mM remove 916,374 - ybjD b0876 0.41 -0.3 916,463 + ybjD b0876 0.46 -0.5 916,463 + ybjD b0876 0.46 -0.0 916,540 - ybjD b0876 0.51 -0.3 916,546 + ybjD b0876 0.51 -0.5 916,717 - ybjD b0876 0.62 +0.9 916,717 - ybjD b0876 0.62 -0.2 916,823 + ybjD b0876 0.68 +0.3 916,823 + ybjD b0876 0.68 -0.5 916,869 + ybjD b0876 0.71 -0.4 916,869 + ybjD b0876 0.71 -0.6 916,989 + ybjD b0876 0.78 -0.1 917,422 - -1.0 917,467 + ybjX b0877 0.12 -1.2 917,467 + ybjX b0877 0.12 -0.5 917,528 + ybjX b0877 0.18 -1.8 917,528 + ybjX b0877 0.18 +0.5 917,643 - ybjX b0877 0.29 -1.4 917,658 - ybjX b0877 0.31 +0.4 917,658 - ybjX b0877 0.31 +0.4 917,775 + ybjX b0877 0.43 -1.2 917,828 + ybjX b0877 0.48 -1.4 917,838 + ybjX b0877 0.49 -0.1 917,878 + ybjX b0877 0.53 +0.6 917,878 + ybjX b0877 0.53 -0.0 917,891 - ybjX b0877 0.54 -1.5 917,948 - ybjX b0877 0.60 -0.9 918,050 - ybjX b0877 0.70 -1.5 918,059 + ybjX b0877 0.71 -0.8 918,271 + -0.8 918,484 + +0.2 918,620 + b0878 b0878 0.15 +0.3 918,789 - b0878 b0878 0.30 +1.1 918,849 - +0.1 918,849 - b0878 b0878 0.35 -0.2 918,985 + b0878 b0878 0.47 -0.8 919,227 - b0878 b0878 0.69 -0.3 919,262 + b0878 b0878 0.72 -0.3 919,262 + b0878 b0878 0.72 +0.0 919,298 - b0878 b0878 0.75 -0.5 919,298 - b0878 b0878 0.75 -1.4 919,343 + b0878 b0878 0.79 +0.7
Or see this region's nucleotide sequence