Experiment: Nalidixic 0.0313 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagD and nagC are separated by 47 nucleotides nagC and nagA are separated by 8 nucleotides
b0675: nagD - UMP phosphatase (NCBI), at 698,797 to 699,549
nagD
b0676: nagC - DNA-binding transcriptional dual regulator, repressor of N-acetylglucosamine (NCBI), at 699,597 to 700,817
nagC
b0677: nagA - N-acetylglucosamine-6-phosphate deacetylase (NCBI), at 700,826 to 701,974
nagA
Position (kb) 699
700
701 Strain fitness (log2 ratio) -2
-1
0
1
2 at 698.720 kb on + strand at 698.737 kb on + strand at 698.745 kb on - strand at 698.763 kb on - strand at 698.857 kb on + strand at 698.920 kb on - strand, within nagD at 698.950 kb on + strand, within nagD at 698.950 kb on + strand, within nagD at 698.951 kb on - strand, within nagD at 699.008 kb on + strand, within nagD at 699.024 kb on + strand, within nagD at 699.028 kb on - strand, within nagD at 699.263 kb on - strand, within nagD at 699.263 kb on - strand, within nagD at 699.285 kb on - strand, within nagD at 699.291 kb on + strand, within nagD at 699.349 kb on - strand, within nagD at 699.349 kb on - strand, within nagD at 699.361 kb on + strand, within nagD at 699.405 kb on - strand, within nagD at 699.405 kb on - strand, within nagD at 699.453 kb on + strand, within nagD at 699.553 kb on - strand at 699.563 kb on - strand at 699.745 kb on - strand, within nagC at 699.760 kb on - strand, within nagC at 699.760 kb on - strand, within nagC at 699.760 kb on - strand, within nagC at 699.819 kb on + strand, within nagC at 699.844 kb on + strand, within nagC at 699.854 kb on + strand, within nagC at 699.939 kb on + strand, within nagC at 699.966 kb on - strand, within nagC at 699.966 kb on - strand, within nagC at 699.968 kb on + strand, within nagC at 700.083 kb on + strand, within nagC at 700.083 kb on + strand, within nagC at 700.132 kb on - strand, within nagC at 700.132 kb on - strand, within nagC at 700.193 kb on - strand, within nagC at 700.193 kb on - strand, within nagC at 700.193 kb on - strand, within nagC at 700.206 kb on - strand, within nagC at 700.206 kb on - strand, within nagC at 700.269 kb on + strand, within nagC at 700.454 kb on + strand, within nagC at 700.486 kb on + strand, within nagC at 700.542 kb on - strand, within nagC at 700.726 kb on - strand at 700.726 kb on - strand at 700.733 kb on + strand at 700.759 kb on + strand at 700.759 kb on + strand at 700.761 kb on + strand at 700.813 kb on - strand at 700.813 kb on - strand at 700.967 kb on - strand, within nagA at 700.977 kb on - strand, within nagA at 701.025 kb on - strand, within nagA at 701.074 kb on - strand, within nagA at 701.191 kb on + strand, within nagA at 701.244 kb on - strand, within nagA at 701.309 kb on - strand, within nagA at 701.309 kb on - strand, within nagA at 701.321 kb on + strand, within nagA at 701.327 kb on - strand, within nagA at 701.347 kb on - strand, within nagA at 701.382 kb on + strand, within nagA at 701.417 kb on + strand, within nagA at 701.498 kb on - strand, within nagA at 701.531 kb on - strand, within nagA at 701.565 kb on + strand, within nagA at 701.565 kb on + strand, within nagA at 701.648 kb on + strand, within nagA at 701.692 kb on + strand, within nagA at 701.694 kb on - strand, within nagA at 701.755 kb on + strand, within nagA at 701.766 kb on + strand, within nagA at 701.805 kb on - strand, within nagA
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.0313 mM remove 698,720 + +0.0 698,737 + +0.1 698,745 - -0.5 698,763 - +0.2 698,857 + +0.1 698,920 - nagD b0675 0.16 +0.1 698,950 + nagD b0675 0.20 -0.1 698,950 + nagD b0675 0.20 +0.5 698,951 - nagD b0675 0.20 +0.1 699,008 + nagD b0675 0.28 +0.1 699,024 + nagD b0675 0.30 -1.0 699,028 - nagD b0675 0.31 +0.8 699,263 - nagD b0675 0.62 -1.5 699,263 - nagD b0675 0.62 +1.6 699,285 - nagD b0675 0.65 -0.1 699,291 + nagD b0675 0.66 +0.6 699,349 - nagD b0675 0.73 -0.2 699,349 - nagD b0675 0.73 -0.1 699,361 + nagD b0675 0.75 -0.9 699,405 - nagD b0675 0.81 -0.5 699,405 - nagD b0675 0.81 -0.7 699,453 + nagD b0675 0.87 +0.5 699,553 - +0.5 699,563 - -0.5 699,745 - nagC b0676 0.12 -1.1 699,760 - nagC b0676 0.13 -1.6 699,760 - nagC b0676 0.13 -0.9 699,760 - nagC b0676 0.13 -1.5 699,819 + nagC b0676 0.18 -1.2 699,844 + nagC b0676 0.20 -2.5 699,854 + nagC b0676 0.21 -0.0 699,939 + nagC b0676 0.28 -0.4 699,966 - nagC b0676 0.30 -0.7 699,966 - nagC b0676 0.30 -1.3 699,968 + nagC b0676 0.30 -1.5 700,083 + nagC b0676 0.40 -0.3 700,083 + nagC b0676 0.40 -0.6 700,132 - nagC b0676 0.44 -0.5 700,132 - nagC b0676 0.44 -0.3 700,193 - nagC b0676 0.49 -0.4 700,193 - nagC b0676 0.49 -0.4 700,193 - nagC b0676 0.49 -0.9 700,206 - nagC b0676 0.50 +0.1 700,206 - nagC b0676 0.50 +0.1 700,269 + nagC b0676 0.55 -0.6 700,454 + nagC b0676 0.70 -1.0 700,486 + nagC b0676 0.73 +0.1 700,542 - nagC b0676 0.77 -1.0 700,726 - +0.3 700,726 - -0.9 700,733 + -1.9 700,759 + -0.7 700,759 + -0.7 700,761 + -0.1 700,813 - -0.0 700,813 - -0.0 700,967 - nagA b0677 0.12 -1.5 700,977 - nagA b0677 0.13 -0.2 701,025 - nagA b0677 0.17 +0.1 701,074 - nagA b0677 0.22 -0.5 701,191 + nagA b0677 0.32 +0.4 701,244 - nagA b0677 0.36 -0.2 701,309 - nagA b0677 0.42 -0.4 701,309 - nagA b0677 0.42 -0.9 701,321 + nagA b0677 0.43 +1.1 701,327 - nagA b0677 0.44 +0.1 701,347 - nagA b0677 0.45 -0.6 701,382 + nagA b0677 0.48 -0.2 701,417 + nagA b0677 0.51 -1.1 701,498 - nagA b0677 0.58 -0.5 701,531 - nagA b0677 0.61 -0.3 701,565 + nagA b0677 0.64 +2.5 701,565 + nagA b0677 0.64 +0.0 701,648 + nagA b0677 0.72 +1.1 701,692 + nagA b0677 0.75 +0.5 701,694 - nagA b0677 0.76 +1.1 701,755 + nagA b0677 0.81 +0.7 701,766 + nagA b0677 0.82 +2.1 701,805 - nagA b0677 0.85 -0.5
Or see this region's nucleotide sequence