Strain Fitness in Escherichia coli BW25113 around b4701

Experiment: Nalidixic 0.0313 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nalidixic 0.0313 mM
2,884,378	+	ygcB	b2761	0.68	+2.3
2,884,378	+	ygcB	b2761	0.68	+0.4
2,884,378	+	ygcB	b2761	0.68	-0.8
2,884,378	+	ygcB	b2761	0.68	+0.0
2,884,378	+	ygcB	b2761	0.68	+1.8
2,884,381	-	ygcB	b2761	0.68	-1.5
2,884,381	-	ygcB	b2761	0.68	+0.6
2,884,400	+	ygcB	b2761	0.68	-0.1
2,884,400	+	ygcB	b2761	0.68	+0.6
2,884,400	+	ygcB	b2761	0.68	-0.5
2,884,425	-	ygcB	b2761	0.69	-0.6
2,884,425	-	ygcB	b2761	0.69	+1.0
2,884,427	-	ygcB	b2761	0.69	-0.6
2,884,427	-	ygcB	b2761	0.69	-1.0
2,884,432	-	ygcB	b2761	0.70	+1.0
2,884,432	-	ygcB	b2761	0.70	+1.0
2,884,435	+	ygcB	b2761	0.70	-0.3
2,884,435	+	ygcB	b2761	0.70	+0.3
2,884,445	-	ygcB	b2761	0.70	-1.0
2,884,461	+	ygcB	b2761	0.71	+0.6
2,884,462	-	ygcB	b2761	0.71	-0.6
2,884,462	-	ygcB	b2761	0.71	+0.0
2,884,477	+	ygcB	b2761	0.71	+0.8
2,884,502	+	ygcB	b2761	0.72	+0.5
2,884,502	+	ygcB	b2761	0.72	-0.2
2,884,506	+	ygcB	b2761	0.72	+0.1
2,884,532	+	ygcB	b2761	0.73	+0.4
2,884,544	-	ygcB	b2761	0.74	+0.4
2,884,545	+	ygcB	b2761	0.74	-1.3
2,884,545	+	ygcB	b2761	0.74	+1.0
2,884,546	-	ygcB	b2761	0.74	-1.0
2,884,548	-	ygcB	b2761	0.74	-0.3
2,884,548	-	ygcB	b2761	0.74	-0.6
2,884,553	-	ygcB	b2761	0.74	+1.7
2,884,584	+	ygcB	b2761	0.75	+0.5
2,884,606	+	ygcB	b2761	0.76	-0.6
2,884,606	+	ygcB	b2761	0.76	+0.5
2,884,667	+	ygcB	b2761	0.78	-1.2
2,884,676	+	ygcB	b2761	0.79	-1.8
2,884,698	+	ygcB	b2761	0.80	-0.1
2,884,699	-	ygcB	b2761	0.80	+0.3
2,884,769	+	ygcB	b2761	0.82	+2.0
2,884,770	+	ygcB	b2761	0.82	+0.0
2,884,770	+	ygcB	b2761	0.82	-0.6
2,884,775	-	ygcB	b2761	0.82	+0.6
2,884,778	+	ygcB	b2761	0.83	-1.0
2,884,778	+	ygcB	b2761	0.83	+1.2
2,884,786	-	ygcB	b2761	0.83	+0.1
2,884,786	-	ygcB	b2761	0.83	-0.9
2,884,793	+	ygcB	b2761	0.83	+0.2
2,884,809	-	ygcB	b2761	0.84	-0.3
2,884,809	-	ygcB	b2761	0.84	+0.4
2,884,811	-	ygcB	b2761	0.84	+0.6
2,884,879	+	ygcB	b2761	0.86	-0.6
2,884,879	+	ygcB	b2761	0.86	+0.0
2,884,890	+	ygcB	b2761	0.87	+1.6
2,884,921	-	ygcB	b2761	0.88	-0.6
2,885,142	+				+1.0
2,885,258	-				+1.0
2,885,310	-				+2.0
2,885,310	-				+1.0
2,885,325	+				+0.0
2,885,328	+				+0.0
2,885,328	+				+1.0
2,885,333	-				+2.6
2,885,345	+				+2.6
2,885,357	-				+0.4
2,885,365	+				+0.0
2,885,365	+				+1.0
2,885,390	-	sokX	b4701	0.25	-0.6
2,885,415	-	sokX	b4701	0.70	-2.0
2,885,443	+				+1.0
2,885,448	-				+1.0
2,885,451	+				+2.6
2,885,451	+				+1.0
2,885,455	+				+0.0
2,885,455	+				+1.0
2,885,456	+				+1.0
2,885,464	-				+1.0
2,885,479	+				+1.0
2,885,479	+				+1.0
2,885,482	+				-0.2
2,885,482	+				+0.0
2,885,484	+				+0.0
2,885,484	+				+0.4
2,885,484	+				+0.1
2,885,573	-				+2.0
2,885,616	-				-0.1
2,885,616	-				+0.2
2,885,616	-				+0.4
2,885,617	-				+0.4
2,885,691	-	cysH	b2762	0.12	+0.1
2,885,697	-	cysH	b2762	0.13	+0.0
2,885,742	-	cysH	b2762	0.19	-0.1
2,885,767	-	cysH	b2762	0.23	-0.6
2,885,767	-	cysH	b2762	0.23	-0.1
2,885,777	-	cysH	b2762	0.24	-0.2
2,885,806	-	cysH	b2762	0.28	+0.3
2,885,807	-	cysH	b2762	0.28	+0.2
2,885,807	-	cysH	b2762	0.28	-0.3
2,885,808	-	cysH	b2762	0.28	+0.6
2,885,812	-	cysH	b2762	0.29	-0.7
2,885,812	-	cysH	b2762	0.29	-0.4
2,885,826	-	cysH	b2762	0.31	+0.9
2,885,854	-	cysH	b2762	0.35	+0.3
2,885,855	-	cysH	b2762	0.35	+0.4
2,885,857	+	cysH	b2762	0.35	+1.0
2,885,877	+	cysH	b2762	0.38	+1.0
2,885,877	-	cysH	b2762	0.38	+0.2
2,885,896	-	cysH	b2762	0.40	+0.9
2,885,913	-	cysH	b2762	0.43	+0.8
2,885,920	-	cysH	b2762	0.44	-0.6
2,885,928	+	cysH	b2762	0.45	+0.4
2,885,977	-	cysH	b2762	0.51	+1.9
2,885,977	-	cysH	b2762	0.51	+0.1
2,886,023	-	cysH	b2762	0.58	-0.7
2,886,035	-				+0.0
2,886,141	+	cysH	b2762	0.74	+0.3
2,886,157	-	cysH	b2762	0.76	-0.4