Strain Fitness in Escherichia coli BW25113 around b4191

Experiment: Trimethoprim 0.0025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyjfO and yjfP are separated by 181 nucleotidesyjfP and ulaR overlap by 4 nucleotidesulaR and yjfR are separated by 107 nucleotides b4189: yjfO - orf, hypothetical protein (VIMSS), at 4,414,464 to 4,414,793 yjfO b4190: yjfP - predicted hydrolase (NCBI), at 4,414,975 to 4,415,724 yjfP b4191: ulaR - DNA-binding transcriptional dual regulator (NCBI), at 4,415,721 to 4,416,476 ulaR b4192: yjfR - orf, hypothetical protein (VIMSS), at 4,416,584 to 4,417,648 yjfR Position (kb) 4415 4416 4417Strain fitness (log2 ratio) -2 -1 0 1at 4414.722 kb on - strand, within yjfOat 4414.746 kb on - strand, within yjfOat 4414.791 kb on - strandat 4414.791 kb on - strandat 4414.795 kb on + strandat 4414.800 kb on - strandat 4414.800 kb on - strandat 4414.825 kb on - strandat 4414.838 kb on - strandat 4414.838 kb on - strandat 4414.885 kb on + strandat 4414.899 kb on + strandat 4414.899 kb on + strandat 4415.030 kb on - strandat 4415.030 kb on - strandat 4415.050 kb on - strand, within yjfPat 4415.058 kb on - strand, within yjfPat 4415.058 kb on - strand, within yjfPat 4415.058 kb on - strand, within yjfPat 4415.086 kb on - strand, within yjfPat 4415.103 kb on - strand, within yjfPat 4415.171 kb on + strand, within yjfPat 4415.171 kb on + strand, within yjfPat 4415.370 kb on + strand, within yjfPat 4415.370 kb on + strand, within yjfPat 4415.399 kb on + strand, within yjfPat 4415.399 kb on + strand, within yjfPat 4415.526 kb on - strand, within yjfPat 4415.562 kb on - strand, within yjfPat 4415.590 kb on - strand, within yjfPat 4415.620 kb on + strand, within yjfPat 4415.620 kb on + strand, within yjfPat 4415.632 kb on + strand, within yjfPat 4415.632 kb on + strand, within yjfPat 4415.657 kb on - strandat 4415.724 kb on - strandat 4415.724 kb on - strandat 4415.767 kb on - strandat 4415.767 kb on - strandat 4415.820 kb on + strand, within ulaRat 4415.879 kb on + strand, within ulaRat 4415.879 kb on + strand, within ulaRat 4415.879 kb on - strand, within ulaRat 4416.008 kb on + strand, within ulaRat 4416.037 kb on + strand, within ulaRat 4416.040 kb on - strand, within ulaRat 4416.050 kb on - strand, within ulaRat 4416.178 kb on - strand, within ulaRat 4416.291 kb on + strand, within ulaRat 4416.291 kb on + strand, within ulaRat 4416.291 kb on + strand, within ulaRat 4416.459 kb on + strandat 4416.555 kb on + strandat 4416.555 kb on + strandat 4416.568 kb on - strandat 4416.590 kb on - strandat 4416.639 kb on - strandat 4416.646 kb on + strandat 4416.653 kb on - strandat 4416.660 kb on + strandat 4416.660 kb on + strandat 4416.736 kb on - strand, within yjfRat 4416.758 kb on - strand, within yjfRat 4416.856 kb on + strand, within yjfRat 4416.956 kb on - strand, within yjfRat 4417.019 kb on - strand, within yjfRat 4417.079 kb on + strand, within yjfRat 4417.087 kb on + strand, within yjfRat 4417.095 kb on - strand, within yjfRat 4417.192 kb on - strand, within yjfRat 4417.192 kb on - strand, within yjfRat 4417.282 kb on + strand, within yjfRat 4417.312 kb on - strand, within yjfRat 4417.312 kb on - strand, within yjfRat 4417.392 kb on + strand, within yjfRat 4417.392 kb on + strand, within yjfRat 4417.407 kb on + strand, within yjfRat 4417.428 kb on + strand, within yjfR

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM
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4,414,722 - yjfO b4189 0.78 +0.4
4,414,746 - yjfO b4189 0.85 -0.5
4,414,791 - +0.3
4,414,791 - +0.5
4,414,795 + -1.5
4,414,800 - -0.4
4,414,800 - -0.1
4,414,825 - +0.0
4,414,838 - +0.4
4,414,838 - -0.8
4,414,885 + -0.1
4,414,899 + -0.4
4,414,899 + +1.4
4,415,030 - +1.2
4,415,030 - -1.1
4,415,050 - yjfP b4190 0.10 -0.2
4,415,058 - yjfP b4190 0.11 +0.5
4,415,058 - yjfP b4190 0.11 +1.2
4,415,058 - yjfP b4190 0.11 -0.3
4,415,086 - yjfP b4190 0.15 -0.1
4,415,103 - yjfP b4190 0.17 -0.7
4,415,171 + yjfP b4190 0.26 -0.7
4,415,171 + yjfP b4190 0.26 -0.4
4,415,370 + yjfP b4190 0.53 -0.1
4,415,370 + yjfP b4190 0.53 -2.6
4,415,399 + yjfP b4190 0.57 +0.1
4,415,399 + yjfP b4190 0.57 -0.9
4,415,526 - yjfP b4190 0.73 +0.1
4,415,562 - yjfP b4190 0.78 -0.1
4,415,590 - yjfP b4190 0.82 -0.5
4,415,620 + yjfP b4190 0.86 -0.4
4,415,620 + yjfP b4190 0.86 -1.8
4,415,632 + yjfP b4190 0.88 +0.2
4,415,632 + yjfP b4190 0.88 +1.2
4,415,657 - -0.4
4,415,724 - -0.4
4,415,724 - +0.4
4,415,767 - +0.2
4,415,767 - +0.6
4,415,820 + ulaR b4191 0.13 -0.2
4,415,879 + ulaR b4191 0.21 +1.2
4,415,879 + ulaR b4191 0.21 +0.5
4,415,879 - ulaR b4191 0.21 -0.4
4,416,008 + ulaR b4191 0.38 -0.2
4,416,037 + ulaR b4191 0.42 +0.6
4,416,040 - ulaR b4191 0.42 +0.2
4,416,050 - ulaR b4191 0.44 +1.0
4,416,178 - ulaR b4191 0.60 +1.2
4,416,291 + ulaR b4191 0.75 +0.5
4,416,291 + ulaR b4191 0.75 +1.2
4,416,291 + ulaR b4191 0.75 +0.2
4,416,459 + -0.6
4,416,555 + +1.4
4,416,555 + -2.4
4,416,568 - -0.1
4,416,590 - +0.3
4,416,639 - +0.6
4,416,646 + -0.7
4,416,653 - +0.7
4,416,660 + -0.7
4,416,660 + +0.3
4,416,736 - yjfR b4192 0.14 +0.3
4,416,758 - yjfR b4192 0.16 -0.3
4,416,856 + yjfR b4192 0.26 +0.3
4,416,956 - yjfR b4192 0.35 -1.5
4,417,019 - yjfR b4192 0.41 -0.0
4,417,079 + yjfR b4192 0.46 +0.3
4,417,087 + yjfR b4192 0.47 -0.1
4,417,095 - yjfR b4192 0.48 +0.9
4,417,192 - yjfR b4192 0.57 -0.1
4,417,192 - yjfR b4192 0.57 +0.2
4,417,282 + yjfR b4192 0.66 -0.9
4,417,312 - yjfR b4192 0.68 -0.1
4,417,312 - yjfR b4192 0.68 -0.6
4,417,392 + yjfR b4192 0.76 -0.1
4,417,392 + yjfR b4192 0.76 -0.4
4,417,407 + yjfR b4192 0.77 +0.2
4,417,428 + yjfR b4192 0.79 +0.2

Or see this region's nucleotide sequence