Strain Fitness in Escherichia coli BW25113 around b2892

Experiment: Trimethoprim 0.0025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntprfB and recJ are separated by 90 nucleotidesrecJ and dsbC are separated by 5 nucleotidesdsbC and xerD are separated by 24 nucleotides b2891: prfB - peptide chain release factor RF-2 (NCBI), at 3,033,206 to 3,034,304 prfB b2892: recJ - ssDNA exonuclease, 5' --> 3'-specific (NCBI), at 3,034,395 to 3,036,128 recJ b2893: dsbC - protein disulfide isomerase II (NCBI), at 3,036,134 to 3,036,844 dsbC b2894: xerD - site-specific tyrosine recombinase XerD (NCBI), at 3,036,869 to 3,037,765 xerD Position (kb) 3034 3035 3036 3037Strain fitness (log2 ratio) -2 -1 0 1 2at 3034.603 kb on - strand, within recJat 3034.661 kb on + strand, within recJat 3034.722 kb on + strand, within recJat 3034.911 kb on - strand, within recJat 3034.946 kb on - strand, within recJat 3035.015 kb on - strand, within recJat 3035.046 kb on + strand, within recJat 3035.110 kb on - strand, within recJat 3035.135 kb on - strand, within recJat 3035.270 kb on + strand, within recJat 3035.433 kb on + strand, within recJat 3035.454 kb on - strand, within recJat 3035.489 kb on + strand, within recJat 3035.680 kb on - strand, within recJat 3035.751 kb on - strand, within recJat 3035.751 kb on - strand, within recJat 3035.754 kb on + strand, within recJat 3035.754 kb on + strand, within recJat 3035.757 kb on - strand, within recJat 3035.762 kb on - strand, within recJat 3035.768 kb on + strand, within recJat 3035.768 kb on + strand, within recJat 3035.776 kb on - strand, within recJat 3035.789 kb on - strand, within recJat 3035.789 kb on - strand, within recJat 3035.802 kb on + strand, within recJat 3035.802 kb on + strand, within recJat 3035.807 kb on + strandat 3035.815 kb on - strand, within recJat 3035.854 kb on - strand, within recJat 3035.854 kb on - strand, within recJat 3035.864 kb on - strand, within recJat 3035.869 kb on - strand, within recJat 3035.906 kb on - strand, within recJat 3035.906 kb on - strandat 3035.960 kb on - strandat 3035.996 kb on - strandat 3035.996 kb on - strandat 3035.996 kb on - strandat 3036.046 kb on - strandat 3036.068 kb on - strandat 3036.229 kb on + strand, within dsbCat 3036.229 kb on - strand, within dsbCat 3036.268 kb on + strand, within dsbCat 3036.457 kb on - strand, within dsbCat 3036.511 kb on + strand, within dsbCat 3036.511 kb on + strand, within dsbCat 3036.613 kb on - strand, within dsbCat 3036.626 kb on - strand, within dsbCat 3036.660 kb on - strand, within dsbCat 3036.660 kb on - strand, within dsbCat 3036.666 kb on + strand, within dsbCat 3036.668 kb on + strand, within dsbCat 3036.761 kb on + strand, within dsbC

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM
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3,034,603 - recJ b2892 0.12 +0.3
3,034,661 + recJ b2892 0.15 -0.5
3,034,722 + recJ b2892 0.19 +0.6
3,034,911 - recJ b2892 0.30 -0.0
3,034,946 - recJ b2892 0.32 +0.5
3,035,015 - recJ b2892 0.36 +0.6
3,035,046 + recJ b2892 0.38 +0.8
3,035,110 - recJ b2892 0.41 +0.7
3,035,135 - recJ b2892 0.43 -0.1
3,035,270 + recJ b2892 0.50 +0.7
3,035,433 + recJ b2892 0.60 +0.8
3,035,454 - recJ b2892 0.61 +0.5
3,035,489 + recJ b2892 0.63 +0.1
3,035,680 - recJ b2892 0.74 +1.7
3,035,751 - recJ b2892 0.78 +1.2
3,035,751 - recJ b2892 0.78 +0.7
3,035,754 + recJ b2892 0.78 +0.9
3,035,754 + recJ b2892 0.78 +1.4
3,035,757 - recJ b2892 0.79 +1.0
3,035,762 - recJ b2892 0.79 +0.4
3,035,768 + recJ b2892 0.79 +0.0
3,035,768 + recJ b2892 0.79 -1.2
3,035,776 - recJ b2892 0.80 +0.8
3,035,789 - recJ b2892 0.80 -2.0
3,035,789 - recJ b2892 0.80 +2.2
3,035,802 + recJ b2892 0.81 +1.2
3,035,802 + recJ b2892 0.81 +0.3
3,035,807 + +1.2
3,035,815 - recJ b2892 0.82 +0.0
3,035,854 - recJ b2892 0.84 -0.1
3,035,854 - recJ b2892 0.84 -0.0
3,035,864 - recJ b2892 0.85 +0.4
3,035,869 - recJ b2892 0.85 +0.3
3,035,906 - recJ b2892 0.87 -0.1
3,035,906 - +1.2
3,035,960 - +0.6
3,035,996 - +0.8
3,035,996 - +0.7
3,035,996 - +0.4
3,036,046 - +0.4
3,036,068 - +0.5
3,036,229 + dsbC b2893 0.13 +0.7
3,036,229 - dsbC b2893 0.13 -1.1
3,036,268 + dsbC b2893 0.19 +0.3
3,036,457 - dsbC b2893 0.45 -1.1
3,036,511 + dsbC b2893 0.53 +0.9
3,036,511 + dsbC b2893 0.53 +1.0
3,036,613 - dsbC b2893 0.67 +0.5
3,036,626 - dsbC b2893 0.69 +0.0
3,036,660 - dsbC b2893 0.74 -1.0
3,036,660 - dsbC b2893 0.74 +0.2
3,036,666 + dsbC b2893 0.75 -0.0
3,036,668 + dsbC b2893 0.75 +0.1
3,036,761 + dsbC b2893 0.88 +0.5

Or see this region's nucleotide sequence