Strain Fitness in Escherichia coli BW25113 around b2699

Experiment: Trimethoprim 0.0025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntalaS and recX are separated by 127 nucleotidesrecX and recA are separated by 68 nucleotidesrecA and ygaD are separated by 79 nucleotidesygaD and mltB are separated by 144 nucleotides b2697: alaS - alanyl-tRNA synthetase (NCBI), at 2,817,403 to 2,820,033 alaS b2698: recX - RecA regulator RecX (NCBI), at 2,820,161 to 2,820,661 recX b2699: recA - recombinase A (NCBI), at 2,820,730 to 2,821,791 recA b2700: ygaD - competence damage-inducible protein A (NCBI), at 2,821,871 to 2,822,368 ygaD b2701: mltB - membrane-bound lytic murein transglycosylase B (NCBI), at 2,822,513 to 2,823,598 mltB Position (kb) 2820 2821 2822Strain fitness (log2 ratio) -1 0 1 2at 2820.070 kb on - strandat 2820.143 kb on + strandat 2820.258 kb on + strand, within recXat 2820.258 kb on + strand, within recXat 2820.457 kb on - strand, within recXat 2820.634 kb on - strandat 2820.929 kb on + strand, within recAat 2821.141 kb on - strand, within recAat 2821.166 kb on - strand, within recAat 2821.522 kb on + strand, within recAat 2821.638 kb on + strand, within recAat 2821.701 kb on - strandat 2821.810 kb on - strandat 2821.810 kb on - strandat 2822.013 kb on + strand, within ygaDat 2822.231 kb on - strand, within ygaDat 2822.232 kb on + strand, within ygaDat 2822.333 kb on - strandat 2822.358 kb on + strandat 2822.437 kb on - strandat 2822.488 kb on + strandat 2822.520 kb on + strandat 2822.520 kb on + strandat 2822.545 kb on - strandat 2822.598 kb on - strandat 2822.641 kb on - strand, within mltBat 2822.668 kb on + strand, within mltBat 2822.764 kb on - strand, within mltBat 2822.765 kb on - strand, within mltB

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM
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2,820,070 - +0.2
2,820,143 + -0.5
2,820,258 + recX b2698 0.19 -0.4
2,820,258 + recX b2698 0.19 -0.7
2,820,457 - recX b2698 0.59 -0.0
2,820,634 - +0.5
2,820,929 + recA b2699 0.19 +1.2
2,821,141 - recA b2699 0.39 +1.2
2,821,166 - recA b2699 0.41 +2.2
2,821,522 + recA b2699 0.75 -1.4
2,821,638 + recA b2699 0.85 +1.2
2,821,701 - -0.4
2,821,810 - -0.4
2,821,810 - -0.4
2,822,013 + ygaD b2700 0.29 -0.1
2,822,231 - ygaD b2700 0.72 -0.2
2,822,232 + ygaD b2700 0.72 +0.5
2,822,333 - -0.1
2,822,358 + -0.7
2,822,437 - +0.1
2,822,488 + -1.5
2,822,520 + -0.3
2,822,520 + +0.9
2,822,545 - -0.1
2,822,598 - -1.0
2,822,641 - mltB b2701 0.12 -0.3
2,822,668 + mltB b2701 0.14 -0.3
2,822,764 - mltB b2701 0.23 +0.6
2,822,765 - mltB b2701 0.23 +0.3

Or see this region's nucleotide sequence