Strain Fitness in Escherichia coli BW25113 around b1754

Experiment: Trimethoprim 0.0025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydjZ and ynjA overlap by 1 nucleotidesynjA and b1754 are separated by 9 nucleotidesb1754 and ynjC overlap by 28 nucleotides b1752: ydjZ - conserved inner membrane protein (NCBI), at 1,832,832 to 1,833,539 ydjZ b1753: ynjA - hypothetical protein (NCBI), at 1,833,539 to 1,834,087 ynjA b1754: b1754 - orf, hypothetical protein (VIMSS), at 1,834,097 to 1,835,263 b1754 b1755: ynjC - fused transporter subunits of ABC superfamily: membrane components (RefSeq), at 1,835,236 to 1,836,771 ynjC Position (kb) 1834 1835 1836Strain fitness (log2 ratio) -2 -1 0 1at 1833.245 kb on + strand, within ydjZat 1833.394 kb on - strand, within ydjZat 1833.430 kb on - strand, within ydjZat 1833.789 kb on + strand, within ynjAat 1833.789 kb on + strand, within ynjAat 1834.210 kb on + strandat 1834.332 kb on - strand, within b1754at 1834.427 kb on + strand, within b1754at 1834.893 kb on + strand, within b1754at 1835.210 kb on + strandat 1835.210 kb on + strandat 1835.245 kb on - strandat 1835.495 kb on + strand, within ynjCat 1835.643 kb on - strand, within ynjCat 1835.649 kb on - strand, within ynjCat 1835.682 kb on + strand, within ynjCat 1835.896 kb on + strand, within ynjCat 1835.896 kb on + strand, within ynjCat 1835.982 kb on - strand, within ynjCat 1835.982 kb on - strand, within ynjCat 1836.212 kb on - strand, within ynjC

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM
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1,833,245 + ydjZ b1752 0.58 -0.3
1,833,394 - ydjZ b1752 0.79 -0.2
1,833,430 - ydjZ b1752 0.84 -2.0
1,833,789 + ynjA b1753 0.46 +0.3
1,833,789 + ynjA b1753 0.46 +0.1
1,834,210 + +0.4
1,834,332 - b1754 b1754 0.20 +0.7
1,834,427 + b1754 b1754 0.28 -0.5
1,834,893 + b1754 b1754 0.68 +0.8
1,835,210 + +0.2
1,835,210 + -0.5
1,835,245 - -0.5
1,835,495 + ynjC b1755 0.17 -0.3
1,835,643 - ynjC b1755 0.26 +0.1
1,835,649 - ynjC b1755 0.27 -0.5
1,835,682 + ynjC b1755 0.29 +0.0
1,835,896 + ynjC b1755 0.43 -0.4
1,835,896 + ynjC b1755 0.43 -1.0
1,835,982 - ynjC b1755 0.49 +0.7
1,835,982 - ynjC b1755 0.49 -0.2
1,836,212 - ynjC b1755 0.64 +0.6

Or see this region's nucleotide sequence