Strain Fitness in Escherichia coli BW25113 around b1063

Experiment: Trimethoprim 0.0025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpyrC and yceB are separated by 105 nucleotidesyceB and grxB are separated by 133 nucleotidesgrxB and yceL are separated by 63 nucleotides b1062: pyrC - dihydroorotase (NCBI), at 1,120,784 to 1,121,830 pyrC b1063: yceB - predicted lipoprotein (NCBI), at 1,121,936 to 1,122,496 yceB b1064: grxB - glutaredoxin 2 (Grx2) (NCBI), at 1,122,630 to 1,123,277 grxB b1065: yceL - orf, hypothetical protein (VIMSS), at 1,123,341 to 1,124,549 yceL Position (kb) 1121 1122 1123Strain fitness (log2 ratio) -1 0 1at 1120.985 kb on - strand, within pyrCat 1120.985 kb on - strand, within pyrCat 1121.019 kb on + strand, within pyrCat 1121.059 kb on + strand, within pyrCat 1121.073 kb on - strand, within pyrCat 1121.073 kb on - strand, within pyrCat 1121.123 kb on + strand, within pyrCat 1121.123 kb on + strand, within pyrCat 1121.174 kb on - strand, within pyrCat 1121.174 kb on - strand, within pyrCat 1121.195 kb on - strand, within pyrCat 1121.205 kb on - strand, within pyrCat 1121.205 kb on - strand, within pyrCat 1121.210 kb on + strand, within pyrCat 1121.427 kb on - strand, within pyrCat 1121.456 kb on + strand, within pyrCat 1121.495 kb on + strand, within pyrCat 1121.574 kb on - strand, within pyrCat 1121.574 kb on - strand, within pyrCat 1121.636 kb on + strand, within pyrCat 1121.641 kb on + strand, within pyrCat 1121.668 kb on + strand, within pyrCat 1121.668 kb on + strand, within pyrCat 1121.689 kb on + strand, within pyrCat 1121.689 kb on + strand, within pyrCat 1121.694 kb on + strand, within pyrCat 1121.793 kb on + strandat 1121.793 kb on + strandat 1122.106 kb on - strand, within yceBat 1122.106 kb on - strand, within yceBat 1122.256 kb on - strand, within yceBat 1122.315 kb on + strand, within yceBat 1122.325 kb on + strand, within yceBat 1122.356 kb on + strand, within yceBat 1122.356 kb on + strand, within yceBat 1122.501 kb on + strandat 1122.533 kb on + strandat 1122.546 kb on - strandat 1122.549 kb on - strandat 1122.556 kb on + strandat 1122.689 kb on + strandat 1122.829 kb on - strand, within grxBat 1122.942 kb on - strand, within grxBat 1123.018 kb on - strand, within grxBat 1123.099 kb on + strand, within grxBat 1123.099 kb on + strand, within grxBat 1123.099 kb on + strand, within grxBat 1123.195 kb on - strand, within grxBat 1123.274 kb on + strandat 1123.322 kb on + strandat 1123.322 kb on + strandat 1123.329 kb on - strandat 1123.443 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM
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1,120,985 - pyrC b1062 0.19 -0.0
1,120,985 - pyrC b1062 0.19 -0.4
1,121,019 + pyrC b1062 0.22 -1.4
1,121,059 + pyrC b1062 0.26 +0.6
1,121,073 - pyrC b1062 0.28 -0.1
1,121,073 - pyrC b1062 0.28 +0.8
1,121,123 + pyrC b1062 0.32 -0.4
1,121,123 + pyrC b1062 0.32 -0.4
1,121,174 - pyrC b1062 0.37 +0.2
1,121,174 - pyrC b1062 0.37 -0.4
1,121,195 - pyrC b1062 0.39 -0.9
1,121,205 - pyrC b1062 0.40 +0.6
1,121,205 - pyrC b1062 0.40 -0.0
1,121,210 + pyrC b1062 0.41 -1.3
1,121,427 - pyrC b1062 0.61 +0.2
1,121,456 + pyrC b1062 0.64 -1.5
1,121,495 + pyrC b1062 0.68 -0.7
1,121,574 - pyrC b1062 0.75 +0.3
1,121,574 - pyrC b1062 0.75 -0.6
1,121,636 + pyrC b1062 0.81 -0.5
1,121,641 + pyrC b1062 0.82 -0.1
1,121,668 + pyrC b1062 0.84 +0.4
1,121,668 + pyrC b1062 0.84 +0.1
1,121,689 + pyrC b1062 0.86 -0.1
1,121,689 + pyrC b1062 0.86 -0.4
1,121,694 + pyrC b1062 0.87 -0.9
1,121,793 + +0.3
1,121,793 + +0.3
1,122,106 - yceB b1063 0.30 +1.6
1,122,106 - yceB b1063 0.30 +0.6
1,122,256 - yceB b1063 0.57 -0.3
1,122,315 + yceB b1063 0.68 +0.7
1,122,325 + yceB b1063 0.69 -0.9
1,122,356 + yceB b1063 0.75 +1.3
1,122,356 + yceB b1063 0.75 +0.3
1,122,501 + -0.5
1,122,533 + -0.3
1,122,546 - -0.9
1,122,549 - -0.3
1,122,556 + -0.2
1,122,689 + -0.2
1,122,829 - grxB b1064 0.31 +0.0
1,122,942 - grxB b1064 0.48 -0.5
1,123,018 - grxB b1064 0.60 +0.2
1,123,099 + grxB b1064 0.72 -0.0
1,123,099 + grxB b1064 0.72 +0.0
1,123,099 + grxB b1064 0.72 +0.2
1,123,195 - grxB b1064 0.87 -0.3
1,123,274 + +0.4
1,123,322 + -0.2
1,123,322 + +0.1
1,123,329 - -1.1
1,123,443 + -0.7

Or see this region's nucleotide sequence