Strain Fitness in Escherichia coli BW25113 around b1020

Experiment: Trimethoprim 0.0025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycdB and phoH are separated by 344 nucleotidesphoH and ycdP are separated by 49 nucleotidesycdP and ycdQ are separated by 1 nucleotides b1019: ycdB - hypothetical protein (NCBI), at 1,082,599 to 1,083,870 ycdB b1020: phoH - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 1,084,215 to 1,085,279 phoH b1021: ycdP - predicted inner membrane protein (NCBI), at 1,085,329 to 1,085,742 ycdP b1022: ycdQ - predicted glycosyl transferase (NCBI), at 1,085,744 to 1,087,069 ycdQ Position (kb) 1084 1085 1086Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1083.620 kb on + strand, within ycdBat 1083.620 kb on + strand, within ycdBat 1083.621 kb on + strand, within ycdBat 1083.650 kb on + strand, within ycdBat 1083.720 kb on - strand, within ycdBat 1083.787 kb on - strandat 1083.904 kb on + strandat 1084.000 kb on - strandat 1084.124 kb on - strandat 1084.407 kb on - strand, within phoHat 1084.446 kb on + strand, within phoHat 1084.494 kb on + strand, within phoHat 1084.561 kb on + strand, within phoHat 1084.620 kb on - strand, within phoHat 1084.765 kb on + strand, within phoHat 1084.860 kb on - strand, within phoHat 1084.946 kb on + strand, within phoHat 1084.999 kb on + strand, within phoHat 1085.048 kb on + strand, within phoHat 1085.201 kb on + strandat 1085.249 kb on + strandat 1085.249 kb on + strandat 1085.268 kb on + strandat 1085.277 kb on - strandat 1085.291 kb on + strandat 1085.292 kb on - strandat 1085.394 kb on + strand, within ycdPat 1085.394 kb on + strand, within ycdPat 1085.394 kb on + strand, within ycdPat 1085.426 kb on - strand, within ycdPat 1085.438 kb on + strand, within ycdPat 1085.438 kb on + strand, within ycdPat 1085.459 kb on - strand, within ycdPat 1085.459 kb on - strand, within ycdPat 1085.461 kb on + strand, within ycdPat 1085.461 kb on + strand, within ycdPat 1085.473 kb on + strand, within ycdPat 1085.473 kb on + strand, within ycdPat 1085.476 kb on + strand, within ycdPat 1085.484 kb on - strand, within ycdPat 1085.507 kb on - strand, within ycdPat 1085.646 kb on - strand, within ycdPat 1085.679 kb on + strand, within ycdPat 1085.759 kb on + strandat 1085.765 kb on + strandat 1085.782 kb on - strandat 1085.784 kb on - strandat 1085.791 kb on + strandat 1085.820 kb on + strandat 1085.823 kb on - strandat 1085.899 kb on + strand, within ycdQat 1085.981 kb on + strand, within ycdQat 1086.064 kb on - strand, within ycdQat 1086.069 kb on - strand, within ycdQat 1086.069 kb on - strand, within ycdQat 1086.069 kb on - strand, within ycdQat 1086.069 kb on - strand, within ycdQat 1086.073 kb on - strand, within ycdQat 1086.171 kb on - strand, within ycdQat 1086.171 kb on - strand, within ycdQat 1086.181 kb on - strand, within ycdQat 1086.181 kb on - strand, within ycdQat 1086.181 kb on - strand, within ycdQat 1086.181 kb on - strand, within ycdQat 1086.181 kb on - strand, within ycdQat 1086.188 kb on - strand, within ycdQat 1086.204 kb on - strand, within ycdQat 1086.204 kb on - strand, within ycdQat 1086.204 kb on - strand, within ycdQat 1086.204 kb on - strand, within ycdQat 1086.239 kb on - strand, within ycdQat 1086.239 kb on - strand, within ycdQat 1086.247 kb on - strand, within ycdQat 1086.247 kb on - strand, within ycdQat 1086.249 kb on - strand, within ycdQat 1086.249 kb on - strand, within ycdQat 1086.249 kb on - strand, within ycdQat 1086.249 kb on - strand, within ycdQat 1086.251 kb on - strand, within ycdQ

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Per-strain Table

Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM
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1,083,620 + ycdB b1019 0.80 +1.6
1,083,620 + ycdB b1019 0.80 -0.5
1,083,621 + ycdB b1019 0.80 +0.6
1,083,650 + ycdB b1019 0.83 -0.5
1,083,720 - ycdB b1019 0.88 -0.7
1,083,787 - -0.2
1,083,904 + -0.3
1,084,000 - -0.6
1,084,124 - -0.4
1,084,407 - phoH b1020 0.18 -0.3
1,084,446 + phoH b1020 0.22 +0.3
1,084,494 + phoH b1020 0.26 -0.0
1,084,561 + phoH b1020 0.32 +0.2
1,084,620 - phoH b1020 0.38 +0.2
1,084,765 + phoH b1020 0.52 +0.6
1,084,860 - phoH b1020 0.61 +0.5
1,084,946 + phoH b1020 0.69 +0.8
1,084,999 + phoH b1020 0.74 -0.1
1,085,048 + phoH b1020 0.78 +1.5
1,085,201 + -0.1
1,085,249 + -0.9
1,085,249 + -0.1
1,085,268 + -0.2
1,085,277 - -3.1
1,085,291 + -0.5
1,085,292 - +0.9
1,085,394 + ycdP b1021 0.16 +0.1
1,085,394 + ycdP b1021 0.16 -1.4
1,085,394 + ycdP b1021 0.16 +0.8
1,085,426 - ycdP b1021 0.23 +0.7
1,085,438 + ycdP b1021 0.26 -1.3
1,085,438 + ycdP b1021 0.26 +1.0
1,085,459 - ycdP b1021 0.31 +3.6
1,085,459 - ycdP b1021 0.31 +0.9
1,085,461 + ycdP b1021 0.32 +1.0
1,085,461 + ycdP b1021 0.32 -0.1
1,085,473 + ycdP b1021 0.35 -0.1
1,085,473 + ycdP b1021 0.35 -1.5
1,085,476 + ycdP b1021 0.36 +1.3
1,085,484 - ycdP b1021 0.37 +0.2
1,085,507 - ycdP b1021 0.43 +0.3
1,085,646 - ycdP b1021 0.77 +1.3
1,085,679 + ycdP b1021 0.85 -0.3
1,085,759 + -0.7
1,085,765 + +0.3
1,085,782 - +0.3
1,085,784 - -0.0
1,085,791 + +1.3
1,085,820 + +0.4
1,085,823 - +1.3
1,085,899 + ycdQ b1022 0.12 +0.3
1,085,981 + ycdQ b1022 0.18 +1.3
1,086,064 - ycdQ b1022 0.24 -0.0
1,086,069 - ycdQ b1022 0.25 +0.3
1,086,069 - ycdQ b1022 0.25 -0.3
1,086,069 - ycdQ b1022 0.25 +2.3
1,086,069 - ycdQ b1022 0.25 +0.1
1,086,073 - ycdQ b1022 0.25 +1.0
1,086,171 - ycdQ b1022 0.32 +1.3
1,086,171 - ycdQ b1022 0.32 +0.7
1,086,181 - ycdQ b1022 0.33 +1.3
1,086,181 - ycdQ b1022 0.33 +1.3
1,086,181 - ycdQ b1022 0.33 +0.3
1,086,181 - ycdQ b1022 0.33 -0.3
1,086,181 - ycdQ b1022 0.33 +1.3
1,086,188 - ycdQ b1022 0.33 -0.5
1,086,204 - ycdQ b1022 0.35 +1.7
1,086,204 - ycdQ b1022 0.35 +0.3
1,086,204 - ycdQ b1022 0.35 +1.3
1,086,204 - ycdQ b1022 0.35 +0.3
1,086,239 - ycdQ b1022 0.37 -0.0
1,086,239 - ycdQ b1022 0.37 -1.1
1,086,247 - ycdQ b1022 0.38 +0.8
1,086,247 - ycdQ b1022 0.38 -0.3
1,086,249 - ycdQ b1022 0.38 +0.7
1,086,249 - ycdQ b1022 0.38 -0.3
1,086,249 - ycdQ b1022 0.38 +0.1
1,086,249 - ycdQ b1022 0.38 +0.3
1,086,251 - ycdQ b1022 0.38 -0.0

Or see this region's nucleotide sequence