Experiment: Trimethoprim 0.0025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysS and ybcI are separated by 35 nucleotides ybcI and ybcJ are separated by 107 nucleotides ybcJ and folD are separated by 1 nucleotides
b0526: cysS - cysteinyl-tRNA synthetase (NCBI), at 553,834 to 555,219
cysS
b0527: ybcI - conserved inner membrane protein (NCBI), at 555,255 to 555,776
ybcI
b0528: ybcJ - orf, hypothetical protein (VIMSS), at 555,884 to 556,096
ybcJ
b0529: folD - bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase (NCBI), at 556,098 to 556,964
folD
Position (kb) 555
556 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 555.277 kb on + strand at 555.277 kb on + strand at 555.306 kb on + strand at 555.340 kb on - strand, within ybcI at 555.425 kb on + strand, within ybcI at 555.455 kb on + strand, within ybcI at 555.553 kb on + strand, within ybcI at 555.629 kb on - strand, within ybcI at 555.663 kb on + strand, within ybcI at 555.761 kb on + strand at 555.815 kb on + strand at 555.823 kb on - strand at 555.895 kb on + strand at 555.898 kb on + strand at 555.902 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trimethoprim 0.0025 mM remove 555,277 + +1.2 555,277 + -1.8 555,306 + -0.3 555,340 - ybcI b0527 0.16 -0.1 555,425 + ybcI b0527 0.33 -0.2 555,455 + ybcI b0527 0.38 +0.1 555,553 + ybcI b0527 0.57 +0.4 555,629 - ybcI b0527 0.72 +0.4 555,663 + ybcI b0527 0.78 +1.3 555,761 + -0.2 555,815 + +0.2 555,823 - -0.5 555,895 + +3.0 555,898 + +2.5 555,902 + +3.2
Or see this region's nucleotide sequence