Experiment: Ciprofloxacin Hydrochloride 0.0003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt spoT and trmH are separated by 6 nucleotides trmH and recG are separated by 5 nucleotides
b3650: spoT - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI), at 3,820,423 to 3,822,531
spoT
b3651: trmH - tRNA (Guanosine-2'-O-)-methyltransferase (NCBI), at 3,822,538 to 3,823,227
trmH
b3652: recG - ATP-dependent DNA helicase (NCBI), at 3,823,233 to 3,825,314
recG
Position (kb)
3822
3823
3824 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 3821.855 kb on + strand, within spoT at 3821.855 kb on + strand, within spoT at 3821.879 kb on + strand, within spoT at 3821.920 kb on - strand, within spoT at 3821.939 kb on + strand, within spoT at 3821.957 kb on - strand, within spoT at 3821.957 kb on - strand, within spoT at 3821.972 kb on - strand, within spoT at 3822.086 kb on + strand, within spoT at 3822.104 kb on - strand, within spoT at 3822.116 kb on - strand, within spoT at 3822.174 kb on - strand, within spoT at 3822.214 kb on - strand, within spoT at 3822.220 kb on + strand, within spoT at 3822.220 kb on + strand, within spoT at 3822.222 kb on + strand, within spoT at 3822.244 kb on + strand, within spoT at 3822.263 kb on - strand, within spoT at 3822.263 kb on - strand, within spoT at 3822.397 kb on - strand at 3822.397 kb on - strand at 3822.401 kb on - strand at 3822.409 kb on - strand at 3822.409 kb on - strand at 3822.417 kb on + strand at 3822.455 kb on + strand at 3822.455 kb on + strand at 3822.493 kb on + strand at 3822.493 kb on + strand at 3822.592 kb on + strand at 3822.615 kb on + strand, within trmH at 3822.615 kb on + strand, within trmH at 3822.615 kb on + strand, within trmH at 3822.618 kb on - strand, within trmH at 3822.635 kb on - strand, within trmH at 3822.733 kb on + strand, within trmH at 3822.746 kb on + strand, within trmH at 3822.746 kb on + strand, within trmH at 3822.749 kb on - strand, within trmH at 3822.761 kb on - strand, within trmH at 3822.778 kb on - strand, within trmH at 3822.796 kb on - strand, within trmH at 3822.799 kb on + strand, within trmH at 3822.799 kb on + strand, within trmH at 3822.807 kb on - strand, within trmH at 3822.922 kb on - strand, within trmH at 3822.930 kb on - strand, within trmH at 3822.930 kb on - strand, within trmH at 3822.945 kb on - strand, within trmH at 3823.040 kb on - strand, within trmH at 3823.089 kb on - strand, within trmH at 3823.117 kb on - strand, within trmH at 3823.179 kb on + strand at 3823.179 kb on + strand at 3823.189 kb on + strand at 3823.189 kb on + strand at 3823.215 kb on - strand at 3823.215 kb on - strand at 3823.231 kb on - strand at 3823.265 kb on + strand at 3823.267 kb on + strand at 3823.285 kb on + strand at 3823.341 kb on + strand at 3823.341 kb on + strand at 3823.379 kb on + strand at 3823.379 kb on + strand at 3823.387 kb on - strand at 3823.387 kb on - strand at 3823.393 kb on + strand at 3823.439 kb on + strand at 3823.482 kb on - strand, within recG at 3823.562 kb on - strand, within recG at 3823.574 kb on + strand, within recG at 3823.582 kb on - strand, within recG at 3823.629 kb on - strand, within recG at 3823.641 kb on + strand, within recG at 3823.649 kb on + strand, within recG at 3823.662 kb on - strand, within recG at 3823.690 kb on - strand, within recG at 3823.690 kb on - strand, within recG at 3823.751 kb on + strand, within recG at 3823.751 kb on + strand, within recG at 3823.776 kb on - strand, within recG at 3823.793 kb on + strand, within recG at 3823.805 kb on + strand, within recG at 3823.823 kb on + strand, within recG at 3823.831 kb on - strand, within recG at 3823.881 kb on - strand, within recG at 3823.883 kb on + strand, within recG at 3823.925 kb on - strand, within recG at 3823.969 kb on + strand, within recG at 3824.052 kb on - strand, within recG at 3824.052 kb on - strand, within recG at 3824.061 kb on + strand, within recG at 3824.085 kb on + strand, within recG at 3824.088 kb on - strand, within recG at 3824.112 kb on + strand, within recG at 3824.142 kb on - strand, within recG at 3824.163 kb on - strand, within recG at 3824.193 kb on + strand, within recG
Per-strain Table
Position Strand Gene LocusTag Fraction Ciprofloxacin Hydrochloride 0.0003 mM remove 3,821,855 + spoT b3650 0.68 -0.6 3,821,855 + spoT b3650 0.68 -1.3 3,821,879 + spoT b3650 0.69 -0.2 3,821,920 - spoT b3650 0.71 -1.1 3,821,939 + spoT b3650 0.72 -2.3 3,821,957 - spoT b3650 0.73 -2.1 3,821,957 - spoT b3650 0.73 -1.1 3,821,972 - spoT b3650 0.73 -2.9 3,822,086 + spoT b3650 0.79 +0.3 3,822,104 - spoT b3650 0.80 +1.2 3,822,116 - spoT b3650 0.80 +0.5 3,822,174 - spoT b3650 0.83 -0.7 3,822,214 - spoT b3650 0.85 -2.7 3,822,220 + spoT b3650 0.85 -2.0 3,822,220 + spoT b3650 0.85 -0.6 3,822,222 + spoT b3650 0.85 -0.7 3,822,244 + spoT b3650 0.86 +0.5 3,822,263 - spoT b3650 0.87 +2.0 3,822,263 - spoT b3650 0.87 +2.0 3,822,397 - +2.2 3,822,397 - -2.1 3,822,401 - +0.5 3,822,409 - -2.1 3,822,409 - -2.1 3,822,417 + -0.6 3,822,455 + -4.1 3,822,455 + -0.6 3,822,493 + -1.7 3,822,493 + -0.1 3,822,592 + +0.7 3,822,615 + trmH b3651 0.11 -0.3 3,822,615 + trmH b3651 0.11 -0.8 3,822,615 + trmH b3651 0.11 -0.1 3,822,618 - trmH b3651 0.12 +1.5 3,822,635 - trmH b3651 0.14 -0.4 3,822,733 + trmH b3651 0.28 -0.5 3,822,746 + trmH b3651 0.30 +1.1 3,822,746 + trmH b3651 0.30 -0.2 3,822,749 - trmH b3651 0.31 +2.4 3,822,761 - trmH b3651 0.32 -0.3 3,822,778 - trmH b3651 0.35 +2.4 3,822,796 - trmH b3651 0.37 +0.9 3,822,799 + trmH b3651 0.38 +1.1 3,822,799 + trmH b3651 0.38 +2.0 3,822,807 - trmH b3651 0.39 +1.1 3,822,922 - trmH b3651 0.56 +1.5 3,822,930 - trmH b3651 0.57 +1.1 3,822,930 - trmH b3651 0.57 +1.9 3,822,945 - trmH b3651 0.59 +1.2 3,823,040 - trmH b3651 0.73 -3.4 3,823,089 - trmH b3651 0.80 +1.5 3,823,117 - trmH b3651 0.84 +1.3 3,823,179 + +0.3 3,823,179 + -0.3 3,823,189 + -1.7 3,823,189 + +3.0 3,823,215 - +2.5 3,823,215 - +1.0 3,823,231 - -0.7 3,823,265 + +0.5 3,823,267 + -0.1 3,823,285 + +0.1 3,823,341 + +2.7 3,823,341 + -0.1 3,823,379 + +2.6 3,823,379 + +3.5 3,823,387 - +2.6 3,823,387 - +2.1 3,823,393 + +0.9 3,823,439 + -0.7 3,823,482 - recG b3652 0.12 +4.9 3,823,562 - recG b3652 0.16 +0.6 3,823,574 + recG b3652 0.16 +1.9 3,823,582 - recG b3652 0.17 +3.1 3,823,629 - recG b3652 0.19 +1.8 3,823,641 + recG b3652 0.20 +1.3 3,823,649 + recG b3652 0.20 +1.7 3,823,662 - recG b3652 0.21 +3.1 3,823,690 - recG b3652 0.22 +0.5 3,823,690 - recG b3652 0.22 -2.7 3,823,751 + recG b3652 0.25 +1.5 3,823,751 + recG b3652 0.25 -0.3 3,823,776 - recG b3652 0.26 +1.0 3,823,793 + recG b3652 0.27 +0.2 3,823,805 + recG b3652 0.27 +1.0 3,823,823 + recG b3652 0.28 +2.5 3,823,831 - recG b3652 0.29 +2.2 3,823,881 - recG b3652 0.31 +4.1 3,823,883 + recG b3652 0.31 +1.9 3,823,925 - recG b3652 0.33 +0.8 3,823,969 + recG b3652 0.35 +0.6 3,824,052 - recG b3652 0.39 -3.4 3,824,052 - recG b3652 0.39 +1.4 3,824,061 + recG b3652 0.40 +0.4 3,824,085 + recG b3652 0.41 +1.1 3,824,088 - recG b3652 0.41 +3.3 3,824,112 + recG b3652 0.42 +0.7 3,824,142 - recG b3652 0.44 +0.9 3,824,163 - recG b3652 0.45 -0.2 3,824,193 + recG b3652 0.46 +2.2
Or see this region's nucleotide sequence