Experiment: Ciprofloxacin Hydrochloride 0.0003 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt add and b1624 are separated by 33 nucleotides b1624 and blr are separated by 242 nucleotides blr and ydgT are separated by 272 nucleotides ydgT and b1626 are separated by 85 nucleotides
b1623: add - adenosine deaminase (NCBI), at 1,700,257 to 1,701,258
add
b1624: b1624 - orf, hypothetical protein (VIMSS), at 1,701,292 to 1,702,332
b1624
b4409: blr - beta-lactam resistance membrane protein (NCBI), at 1,702,575 to 1,702,700
blr
b1625: ydgT - predicted regulator (NCBI), at 1,702,973 to 1,703,188
ydgT
b1626: b1626 - orf, hypothetical protein (VIMSS), at 1,703,274 to 1,703,714
b1626
Position (kb)
1701
1702
1703 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1700.313 kb on - strand at 1700.327 kb on + strand at 1700.572 kb on - strand, within add at 1700.727 kb on + strand, within add at 1700.929 kb on - strand, within add at 1701.020 kb on + strand, within add at 1701.061 kb on - strand, within add at 1701.086 kb on - strand, within add at 1701.086 kb on - strand, within add at 1701.146 kb on + strand, within add at 1701.449 kb on + strand, within b1624 at 1701.449 kb on + strand, within b1624 at 1701.496 kb on - strand, within b1624 at 1701.496 kb on - strand, within b1624 at 1701.536 kb on + strand, within b1624 at 1701.612 kb on - strand, within b1624 at 1701.697 kb on + strand, within b1624 at 1701.798 kb on + strand, within b1624 at 1701.798 kb on + strand, within b1624 at 1701.813 kb on - strand, within b1624 at 1701.813 kb on - strand, within b1624 at 1701.886 kb on - strand, within b1624 at 1701.923 kb on + strand, within b1624 at 1701.972 kb on - strand, within b1624 at 1701.988 kb on + strand, within b1624 at 1701.988 kb on + strand, within b1624 at 1702.175 kb on - strand, within b1624 at 1702.296 kb on - strand at 1702.375 kb on + strand at 1702.382 kb on - strand at 1702.402 kb on - strand at 1702.418 kb on - strand at 1702.423 kb on + strand at 1702.425 kb on + strand at 1702.425 kb on + strand at 1702.541 kb on - strand at 1702.546 kb on - strand at 1702.553 kb on + strand at 1702.553 kb on + strand at 1702.560 kb on - strand at 1702.565 kb on - strand at 1702.569 kb on + strand at 1702.569 kb on - strand at 1702.569 kb on - strand at 1702.572 kb on + strand at 1702.626 kb on + strand, within blr at 1702.634 kb on - strand, within blr at 1702.638 kb on - strand, within blr at 1702.648 kb on + strand, within blr at 1702.651 kb on - strand, within blr at 1702.657 kb on + strand, within blr at 1702.661 kb on + strand, within blr at 1702.666 kb on + strand, within blr at 1702.671 kb on + strand, within blr at 1702.671 kb on + strand, within blr at 1702.673 kb on + strand, within blr at 1702.679 kb on - strand, within blr at 1702.694 kb on + strand at 1702.694 kb on - strand at 1702.715 kb on + strand at 1702.725 kb on + strand at 1702.725 kb on + strand at 1702.725 kb on + strand at 1702.812 kb on + strand at 1702.812 kb on + strand at 1702.821 kb on + strand at 1702.979 kb on + strand at 1702.984 kb on + strand at 1702.984 kb on + strand at 1702.987 kb on - strand at 1702.987 kb on - strand at 1702.987 kb on - strand at 1702.990 kb on - strand at 1702.992 kb on - strand at 1703.033 kb on - strand, within ydgT at 1703.052 kb on + strand, within ydgT at 1703.054 kb on - strand, within ydgT at 1703.054 kb on - strand, within ydgT at 1703.055 kb on + strand, within ydgT at 1703.070 kb on + strand, within ydgT at 1703.070 kb on + strand, within ydgT at 1703.070 kb on + strand, within ydgT at 1703.070 kb on + strand, within ydgT at 1703.070 kb on + strand, within ydgT at 1703.078 kb on - strand, within ydgT at 1703.078 kb on - strand, within ydgT at 1703.081 kb on + strand, within ydgT at 1703.084 kb on + strand, within ydgT at 1703.089 kb on + strand, within ydgT at 1703.092 kb on - strand, within ydgT at 1703.102 kb on - strand, within ydgT at 1703.105 kb on - strand, within ydgT at 1703.109 kb on + strand, within ydgT at 1703.109 kb on + strand, within ydgT at 1703.142 kb on - strand, within ydgT at 1703.153 kb on - strand, within ydgT at 1703.178 kb on + strand at 1703.210 kb on - strand at 1703.263 kb on - strand at 1703.268 kb on - strand at 1703.276 kb on + strand at 1703.278 kb on - strand at 1703.296 kb on + strand at 1703.296 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ciprofloxacin Hydrochloride 0.0003 mM remove 1,700,313 - +1.2 1,700,327 + -2.4 1,700,572 - add b1623 0.31 -0.5 1,700,727 + add b1623 0.47 +0.0 1,700,929 - add b1623 0.67 -0.3 1,701,020 + add b1623 0.76 -0.6 1,701,061 - add b1623 0.80 -1.0 1,701,086 - add b1623 0.83 -1.4 1,701,086 - add b1623 0.83 -2.7 1,701,146 + add b1623 0.89 -0.5 1,701,449 + b1624 b1624 0.15 -2.5 1,701,449 + b1624 b1624 0.15 -1.9 1,701,496 - b1624 b1624 0.20 +0.9 1,701,496 - b1624 b1624 0.20 -3.0 1,701,536 + b1624 b1624 0.23 -0.2 1,701,612 - b1624 b1624 0.31 -0.5 1,701,697 + b1624 b1624 0.39 -0.3 1,701,798 + b1624 b1624 0.49 -1.9 1,701,798 + b1624 b1624 0.49 -1.9 1,701,813 - b1624 b1624 0.50 -0.8 1,701,813 - b1624 b1624 0.50 +1.0 1,701,886 - b1624 b1624 0.57 -1.4 1,701,923 + b1624 b1624 0.61 -2.3 1,701,972 - b1624 b1624 0.65 -3.1 1,701,988 + b1624 b1624 0.67 +0.6 1,701,988 + b1624 b1624 0.67 -1.7 1,702,175 - b1624 b1624 0.85 -1.6 1,702,296 - -0.1 1,702,375 + -0.4 1,702,382 - -0.9 1,702,402 - +1.4 1,702,418 - +1.4 1,702,423 + -0.1 1,702,425 + +0.4 1,702,425 + +1.0 1,702,541 - -0.1 1,702,546 - -0.6 1,702,553 + +0.8 1,702,553 + +3.8 1,702,560 - +4.0 1,702,565 - +2.2 1,702,569 + +0.4 1,702,569 - +1.4 1,702,569 - +1.4 1,702,572 + -3.5 1,702,626 + blr b4409 0.40 +0.2 1,702,634 - blr b4409 0.47 +1.4 1,702,638 - blr b4409 0.50 +1.4 1,702,648 + blr b4409 0.58 -2.6 1,702,651 - blr b4409 0.60 +3.2 1,702,657 + blr b4409 0.65 -0.5 1,702,661 + blr b4409 0.68 +0.9 1,702,666 + blr b4409 0.72 -0.7 1,702,671 + blr b4409 0.76 -0.9 1,702,671 + blr b4409 0.76 +1.5 1,702,673 + blr b4409 0.78 +0.2 1,702,679 - blr b4409 0.83 +1.4 1,702,694 + -1.1 1,702,694 - +1.4 1,702,715 + +0.5 1,702,725 + -4.2 1,702,725 + +0.6 1,702,725 + -2.3 1,702,812 + +0.4 1,702,812 + +0.6 1,702,821 + +0.4 1,702,979 + +0.4 1,702,984 + -0.9 1,702,984 + -1.1 1,702,987 - -2.4 1,702,987 - +0.3 1,702,987 - -1.6 1,702,990 - -2.1 1,702,992 - -2.0 1,703,033 - ydgT b1625 0.28 -0.1 1,703,052 + ydgT b1625 0.37 -0.4 1,703,054 - ydgT b1625 0.38 -2.7 1,703,054 - ydgT b1625 0.38 +0.4 1,703,055 + ydgT b1625 0.38 +2.4 1,703,070 + ydgT b1625 0.45 -0.9 1,703,070 + ydgT b1625 0.45 +1.0 1,703,070 + ydgT b1625 0.45 +1.6 1,703,070 + ydgT b1625 0.45 -0.2 1,703,070 + ydgT b1625 0.45 -2.1 1,703,078 - ydgT b1625 0.49 -1.1 1,703,078 - ydgT b1625 0.49 +0.2 1,703,081 + ydgT b1625 0.50 +0.4 1,703,084 + ydgT b1625 0.51 +0.1 1,703,089 + ydgT b1625 0.54 -0.4 1,703,092 - ydgT b1625 0.55 -3.4 1,703,102 - ydgT b1625 0.60 +1.0 1,703,105 - ydgT b1625 0.61 +0.7 1,703,109 + ydgT b1625 0.63 +1.7 1,703,109 + ydgT b1625 0.63 +0.6 1,703,142 - ydgT b1625 0.78 -0.9 1,703,153 - ydgT b1625 0.83 -0.1 1,703,178 + -2.1 1,703,210 - +0.3 1,703,263 - -1.7 1,703,268 - +0.9 1,703,276 + -1.2 1,703,278 - -0.3 1,703,296 + +0.6 1,703,296 + -1.1
Or see this region's nucleotide sequence