Strain Fitness in Escherichia coli BW25113 around b1474

Experiment: Ciprofloxacin Hydrochloride 0.0003 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyddG and fdnG are separated by 231 nucleotidesfdnG and fdnH are separated by 12 nucleotidesfdnH and fdnI overlap by 8 nucleotides b1473: yddG - predicted methyl viologen efflux pump (RefSeq), at 1,544,312 to 1,545,193 yddG b1474: fdnG - formate dehydrogenase-N, alpha subunit, nitrate-inducible (NCBI), at 1,545,425 to 1,548,472 fdnG b1475: fdnH - formate dehydrogenase-N, Fe-S (beta) subunit, nitrate-inducible (NCBI), at 1,548,485 to 1,549,369 fdnH b1476: fdnI - formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI), at 1,549,362 to 1,550,015 fdnI Position (kb) 1545 1546 1547 1548 1549Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 1544.439 kb on - strand, within yddGat 1544.607 kb on - strand, within yddGat 1544.607 kb on - strand, within yddGat 1544.607 kb on - strand, within yddGat 1544.621 kb on - strand, within yddGat 1544.681 kb on - strand, within yddGat 1544.681 kb on - strand, within yddGat 1544.693 kb on - strand, within yddGat 1544.693 kb on - strand, within yddGat 1544.722 kb on - strand, within yddGat 1544.722 kb on - strand, within yddGat 1544.774 kb on - strand, within yddGat 1544.788 kb on - strand, within yddGat 1544.793 kb on - strand, within yddGat 1544.893 kb on - strand, within yddGat 1544.893 kb on - strand, within yddGat 1544.900 kb on - strand, within yddGat 1544.900 kb on - strand, within yddGat 1544.909 kb on - strand, within yddGat 1545.074 kb on + strand, within yddGat 1545.115 kb on - strandat 1545.117 kb on - strandat 1545.151 kb on - strandat 1545.151 kb on - strandat 1545.163 kb on + strandat 1545.164 kb on + strandat 1545.477 kb on + strandat 1545.491 kb on + strandat 1545.572 kb on + strandat 1546.336 kb on - strand, within fdnGat 1546.336 kb on - strand, within fdnGat 1546.566 kb on - strand, within fdnGat 1546.601 kb on + strand, within fdnGat 1546.601 kb on + strand, within fdnGat 1546.725 kb on - strand, within fdnGat 1546.767 kb on - strand, within fdnGat 1546.767 kb on - strand, within fdnGat 1546.776 kb on - strand, within fdnGat 1546.790 kb on + strand, within fdnGat 1547.224 kb on - strand, within fdnGat 1547.409 kb on + strand, within fdnGat 1547.748 kb on + strand, within fdnGat 1547.748 kb on + strand, within fdnGat 1548.012 kb on + strand, within fdnGat 1548.097 kb on + strand, within fdnGat 1548.097 kb on + strand, within fdnGat 1548.098 kb on + strand, within fdnGat 1548.444 kb on - strandat 1548.444 kb on - strandat 1548.465 kb on + strandat 1548.607 kb on - strand, within fdnHat 1548.745 kb on - strand, within fdnHat 1548.745 kb on - strand, within fdnHat 1548.789 kb on + strand, within fdnHat 1548.789 kb on + strand, within fdnHat 1549.042 kb on - strand, within fdnHat 1549.104 kb on + strand, within fdnHat 1549.104 kb on + strand, within fdnHat 1549.200 kb on - strand, within fdnHat 1549.396 kb on - strandat 1549.396 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ciprofloxacin Hydrochloride 0.0003 mM
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1,544,439 - yddG b1473 0.14 -0.1
1,544,607 - yddG b1473 0.33 +0.5
1,544,607 - yddG b1473 0.33 -1.0
1,544,607 - yddG b1473 0.33 -0.3
1,544,621 - yddG b1473 0.35 +1.8
1,544,681 - yddG b1473 0.42 -1.9
1,544,681 - yddG b1473 0.42 +1.1
1,544,693 - yddG b1473 0.43 +0.4
1,544,693 - yddG b1473 0.43 +0.2
1,544,722 - yddG b1473 0.46 -0.1
1,544,722 - yddG b1473 0.46 +2.7
1,544,774 - yddG b1473 0.52 -0.3
1,544,788 - yddG b1473 0.54 +0.3
1,544,793 - yddG b1473 0.55 -1.5
1,544,893 - yddG b1473 0.66 -1.1
1,544,893 - yddG b1473 0.66 -2.1
1,544,900 - yddG b1473 0.67 -0.5
1,544,900 - yddG b1473 0.67 +1.5
1,544,909 - yddG b1473 0.68 +0.7
1,545,074 + yddG b1473 0.86 -2.3
1,545,115 - -0.1
1,545,117 - -1.5
1,545,151 - -2.4
1,545,151 - -1.7
1,545,163 + -1.0
1,545,164 + +0.8
1,545,477 + +1.3
1,545,491 + +1.7
1,545,572 + +0.5
1,546,336 - fdnG b1474 0.30 -2.4
1,546,336 - fdnG b1474 0.30 -2.5
1,546,566 - fdnG b1474 0.37 -1.3
1,546,601 + fdnG b1474 0.39 -1.3
1,546,601 + fdnG b1474 0.39 +0.5
1,546,725 - fdnG b1474 0.43 -1.4
1,546,767 - fdnG b1474 0.44 -2.0
1,546,767 - fdnG b1474 0.44 +0.3
1,546,776 - fdnG b1474 0.44 -3.8
1,546,790 + fdnG b1474 0.45 -1.0
1,547,224 - fdnG b1474 0.59 +1.3
1,547,409 + fdnG b1474 0.65 -3.6
1,547,748 + fdnG b1474 0.76 +0.6
1,547,748 + fdnG b1474 0.76 -1.3
1,548,012 + fdnG b1474 0.85 -0.3
1,548,097 + fdnG b1474 0.88 -2.8
1,548,097 + fdnG b1474 0.88 -2.1
1,548,098 + fdnG b1474 0.88 -0.9
1,548,444 - -1.8
1,548,444 - +3.8
1,548,465 + -1.3
1,548,607 - fdnH b1475 0.14 -0.5
1,548,745 - fdnH b1475 0.29 +1.2
1,548,745 - fdnH b1475 0.29 +0.9
1,548,789 + fdnH b1475 0.34 -3.0
1,548,789 + fdnH b1475 0.34 +0.9
1,549,042 - fdnH b1475 0.63 -1.5
1,549,104 + fdnH b1475 0.70 +0.1
1,549,104 + fdnH b1475 0.70 -1.5
1,549,200 - fdnH b1475 0.81 -0.4
1,549,396 - +2.3
1,549,396 - -4.1

Or see this region's nucleotide sequence