Experiment: Stem-Atlantic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS03010 and DDA3937_RS03015 are separated by 265 nucleotides DDA3937_RS03015 and DDA3937_RS03020 are separated by 125 nucleotides DDA3937_RS03020 and DDA3937_RS03025 are separated by 158 nucleotides DDA3937_RS03025 and DDA3937_RS03030 are separated by 47 nucleotides
DDA3937_RS03010: DDA3937_RS03010 - serine-type D-Ala-D-Ala carboxypeptidase, at 664,929 to 666,362
_RS03010
DDA3937_RS03015: DDA3937_RS03015 - transcription elongation factor GreA, at 666,628 to 667,104
_RS03015
DDA3937_RS03020: DDA3937_RS03020 - ribosome assembly RNA-binding protein YhbY, at 667,230 to 667,523
_RS03020
DDA3937_RS03025: DDA3937_RS03025 - 23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE, at 667,682 to 668,311
_RS03025
DDA3937_RS03030: DDA3937_RS03030 - ATP-dependent zinc metalloprotease FtsH, at 668,359 to 670,311
_RS03030
Position (kb) 667
668 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 666.277 kb on + strand at 666.277 kb on + strand at 666.277 kb on + strand at 666.277 kb on + strand at 666.277 kb on + strand at 666.277 kb on + strand at 666.277 kb on + strand at 666.278 kb on - strand at 666.278 kb on - strand at 666.278 kb on - strand at 666.286 kb on + strand at 666.286 kb on + strand at 666.286 kb on + strand at 666.286 kb on + strand at 666.287 kb on - strand at 666.287 kb on - strand at 666.287 kb on - strand at 666.324 kb on + strand at 666.324 kb on + strand at 666.325 kb on - strand at 666.325 kb on - strand at 666.325 kb on - strand at 666.325 kb on - strand at 666.325 kb on - strand at 666.329 kb on - strand at 666.372 kb on + strand at 666.372 kb on + strand at 666.373 kb on - strand at 666.373 kb on - strand at 666.376 kb on + strand at 666.376 kb on + strand at 666.376 kb on + strand at 666.377 kb on - strand at 666.377 kb on - strand at 666.377 kb on - strand at 666.397 kb on + strand at 666.397 kb on + strand at 666.398 kb on - strand at 666.398 kb on - strand at 666.400 kb on + strand at 666.400 kb on + strand at 666.401 kb on - strand at 666.423 kb on - strand at 666.451 kb on + strand at 666.452 kb on - strand at 666.511 kb on + strand at 666.521 kb on + strand at 666.569 kb on + strand at 666.611 kb on + strand at 666.617 kb on + strand at 666.617 kb on + strand at 666.627 kb on + strand at 666.715 kb on - strand, within DDA3937_RS03015 at 666.941 kb on - strand at 667.096 kb on + strand at 667.097 kb on - strand at 667.110 kb on - strand at 667.270 kb on + strand, within DDA3937_RS03020 at 667.270 kb on + strand, within DDA3937_RS03020 at 667.270 kb on + strand, within DDA3937_RS03020 at 667.270 kb on + strand, within DDA3937_RS03020 at 667.511 kb on + strand at 667.564 kb on + strand at 667.598 kb on + strand at 667.598 kb on + strand at 667.642 kb on + strand at 667.642 kb on + strand at 667.642 kb on + strand at 667.642 kb on + strand at 667.642 kb on + strand at 667.642 kb on + strand at 667.642 kb on + strand at 667.642 kb on + strand at 667.646 kb on + strand at 667.646 kb on + strand at 667.646 kb on + strand at 667.646 kb on + strand at 667.646 kb on + strand at 667.646 kb on + strand at 667.652 kb on + strand at 667.668 kb on + strand at 667.668 kb on + strand at 667.883 kb on + strand, within DDA3937_RS03025 at 668.105 kb on + strand, within DDA3937_RS03025 at 668.128 kb on + strand, within DDA3937_RS03025 at 668.282 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Atlantic remove 666,277 + +0.4 666,277 + -0.2 666,277 + -1.1 666,277 + -0.4 666,277 + -0.3 666,277 + -0.3 666,277 + -2.0 666,278 - +1.1 666,278 - -2.3 666,278 - +0.6 666,286 + -0.2 666,286 + +0.3 666,286 + -0.0 666,286 + +1.4 666,287 - -1.1 666,287 - +0.3 666,287 - +1.0 666,324 + -1.3 666,324 + -0.5 666,325 - -1.8 666,325 - +1.0 666,325 - -0.8 666,325 - +3.0 666,325 - -3.2 666,329 - +1.2 666,372 + -0.6 666,372 + -0.2 666,373 - -2.0 666,373 - +2.6 666,376 + -2.0 666,376 + +1.6 666,376 + -2.6 666,377 - -0.2 666,377 - +0.4 666,377 - +0.9 666,397 + -1.2 666,397 + -1.0 666,398 - +0.7 666,398 - -0.4 666,400 + +0.5 666,400 + -0.4 666,401 - -0.3 666,423 - +0.5 666,451 + +0.0 666,452 - +0.9 666,511 + -2.4 666,521 + -1.4 666,569 + -0.6 666,611 + -0.1 666,617 + -0.8 666,617 + -0.8 666,627 + -0.8 666,715 - DDA3937_RS03015 0.18 +0.6 666,941 - +0.2 667,096 + +1.6 667,097 - -0.8 667,110 - -0.3 667,270 + DDA3937_RS03020 0.14 -1.3 667,270 + DDA3937_RS03020 0.14 -0.8 667,270 + DDA3937_RS03020 0.14 -1.4 667,270 + DDA3937_RS03020 0.14 +0.3 667,511 + -1.7 667,564 + +1.9 667,598 + +0.5 667,598 + +0.0 667,642 + +0.5 667,642 + -0.9 667,642 + -1.2 667,642 + -0.6 667,642 + +1.0 667,642 + -2.0 667,642 + -0.8 667,642 + -2.0 667,646 + -1.5 667,646 + +1.5 667,646 + -3.1 667,646 + -1.9 667,646 + -2.9 667,646 + +0.1 667,652 + +0.7 667,668 + -0.4 667,668 + -2.0 667,883 + DDA3937_RS03025 0.32 +0.8 668,105 + DDA3937_RS03025 0.67 -0.4 668,128 + DDA3937_RS03025 0.71 -2.3 668,282 + +3.1
Or see this region's nucleotide sequence