Experiment: Stem-Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS05535 and DDA3937_RS05540 overlap by 4 nucleotides DDA3937_RS05540 and DDA3937_RS05545 are separated by 266 nucleotides DDA3937_RS05545 and DDA3937_RS05550 are separated by 65 nucleotides DDA3937_RS05550 and DDA3937_RS05555 overlap by 38 nucleotides
DDA3937_RS05535: DDA3937_RS05535 - (2E,6E)-farnesyl diphosphate synthase, at 1,237,397 to 1,238,308
_RS05535
DDA3937_RS05540: DDA3937_RS05540 - exodeoxyribonuclease VII small subunit, at 1,238,305 to 1,238,553
_RS05540
DDA3937_RS05545: DDA3937_RS05545 - tRNA 4-thiouridine(8) synthase ThiI, at 1,238,820 to 1,240,268
_RS05545
DDA3937_RS05550: DDA3937_RS05550 - protein deglycase YajL, at 1,240,334 to 1,240,930
_RS05550
DDA3937_RS05555: DDA3937_RS05555 - 2-dehydropantoate 2-reductase, at 1,240,893 to 1,241,813
_RS05555
Position (kb)
1238
1239
1240
1241 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1238.575 kb on - strand at 1238.575 kb on - strand at 1238.575 kb on - strand at 1238.577 kb on - strand at 1238.639 kb on - strand at 1238.639 kb on - strand at 1238.639 kb on - strand at 1238.647 kb on - strand at 1238.668 kb on + strand at 1238.669 kb on - strand at 1238.669 kb on - strand at 1238.669 kb on - strand at 1238.677 kb on - strand at 1238.677 kb on - strand at 1238.677 kb on - strand at 1238.679 kb on - strand at 1238.679 kb on - strand at 1238.679 kb on - strand at 1238.679 kb on - strand at 1238.679 kb on - strand at 1238.698 kb on - strand at 1238.741 kb on - strand at 1238.747 kb on - strand at 1238.806 kb on + strand at 1238.807 kb on - strand at 1238.928 kb on + strand at 1238.928 kb on + strand at 1238.928 kb on + strand at 1238.928 kb on + strand at 1238.928 kb on + strand at 1238.929 kb on - strand at 1238.929 kb on - strand at 1238.967 kb on - strand, within DDA3937_RS05545 at 1239.026 kb on + strand, within DDA3937_RS05545 at 1239.026 kb on + strand, within DDA3937_RS05545 at 1239.026 kb on + strand, within DDA3937_RS05545 at 1239.044 kb on + strand, within DDA3937_RS05545 at 1239.044 kb on + strand, within DDA3937_RS05545 at 1239.045 kb on - strand, within DDA3937_RS05545 at 1239.045 kb on - strand, within DDA3937_RS05545 at 1239.069 kb on + strand, within DDA3937_RS05545 at 1239.198 kb on + strand, within DDA3937_RS05545 at 1239.224 kb on + strand, within DDA3937_RS05545 at 1239.225 kb on - strand at 1239.225 kb on - strand, within DDA3937_RS05545 at 1239.315 kb on + strand, within DDA3937_RS05545 at 1239.402 kb on + strand, within DDA3937_RS05545 at 1239.402 kb on + strand, within DDA3937_RS05545 at 1239.402 kb on + strand, within DDA3937_RS05545 at 1239.402 kb on + strand, within DDA3937_RS05545 at 1239.402 kb on + strand, within DDA3937_RS05545 at 1239.402 kb on + strand, within DDA3937_RS05545 at 1239.403 kb on - strand, within DDA3937_RS05545 at 1239.403 kb on - strand, within DDA3937_RS05545 at 1239.403 kb on - strand, within DDA3937_RS05545 at 1239.403 kb on - strand, within DDA3937_RS05545 at 1239.403 kb on - strand, within DDA3937_RS05545 at 1239.403 kb on - strand, within DDA3937_RS05545 at 1239.435 kb on + strand, within DDA3937_RS05545 at 1239.436 kb on - strand at 1239.495 kb on + strand, within DDA3937_RS05545 at 1239.496 kb on - strand, within DDA3937_RS05545 at 1239.496 kb on - strand, within DDA3937_RS05545 at 1239.654 kb on + strand, within DDA3937_RS05545 at 1239.735 kb on - strand, within DDA3937_RS05545 at 1239.779 kb on + strand, within DDA3937_RS05545 at 1239.788 kb on + strand, within DDA3937_RS05545 at 1239.788 kb on + strand, within DDA3937_RS05545 at 1239.789 kb on - strand, within DDA3937_RS05545 at 1239.789 kb on - strand, within DDA3937_RS05545 at 1239.789 kb on - strand, within DDA3937_RS05545 at 1240.129 kb on - strand at 1240.173 kb on + strand at 1240.173 kb on + strand at 1240.173 kb on + strand at 1240.221 kb on + strand at 1240.258 kb on - strand at 1240.350 kb on + strand at 1240.350 kb on + strand at 1240.351 kb on - strand at 1240.356 kb on + strand at 1240.356 kb on + strand at 1240.357 kb on - strand at 1240.357 kb on - strand at 1240.402 kb on + strand, within DDA3937_RS05550 at 1240.402 kb on + strand, within DDA3937_RS05550 at 1240.402 kb on + strand, within DDA3937_RS05550 at 1240.403 kb on - strand, within DDA3937_RS05550 at 1240.561 kb on + strand, within DDA3937_RS05550 at 1240.561 kb on + strand, within DDA3937_RS05550 at 1240.561 kb on + strand, within DDA3937_RS05550 at 1240.562 kb on - strand, within DDA3937_RS05550 at 1240.562 kb on - strand, within DDA3937_RS05550 at 1240.562 kb on - strand, within DDA3937_RS05550 at 1240.562 kb on - strand, within DDA3937_RS05550 at 1240.562 kb on - strand, within DDA3937_RS05550 at 1240.721 kb on + strand, within DDA3937_RS05550 at 1240.895 kb on - strand at 1240.931 kb on - strand at 1240.931 kb on - strand at 1240.931 kb on - strand at 1240.931 kb on - strand at 1240.931 kb on - strand at 1240.952 kb on + strand at 1240.953 kb on - strand at 1240.954 kb on + strand at 1240.954 kb on + strand at 1240.954 kb on + strand at 1240.954 kb on + strand at 1240.955 kb on - strand at 1240.955 kb on - strand at 1240.987 kb on - strand, within DDA3937_RS05555 at 1241.018 kb on - strand, within DDA3937_RS05555 at 1241.029 kb on + strand, within DDA3937_RS05555 at 1241.030 kb on - strand, within DDA3937_RS05555 at 1241.030 kb on - strand, within DDA3937_RS05555 at 1241.117 kb on - strand, within DDA3937_RS05555 at 1241.117 kb on - strand, within DDA3937_RS05555 at 1241.117 kb on - strand, within DDA3937_RS05555 at 1241.141 kb on + strand, within DDA3937_RS05555 at 1241.142 kb on - strand, within DDA3937_RS05555 at 1241.142 kb on - strand, within DDA3937_RS05555 at 1241.142 kb on - strand, within DDA3937_RS05555
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Norland remove 1,238,575 - +1.7 1,238,575 - +1.9 1,238,575 - -0.6 1,238,577 - +1.2 1,238,639 - +2.9 1,238,639 - +1.2 1,238,639 - -2.0 1,238,647 - +0.0 1,238,668 + +0.2 1,238,669 - +2.8 1,238,669 - +0.3 1,238,669 - -1.1 1,238,677 - +0.7 1,238,677 - -1.3 1,238,677 - -1.3 1,238,679 - -0.7 1,238,679 - -0.6 1,238,679 - -1.4 1,238,679 - -0.1 1,238,679 - -1.0 1,238,698 - +0.5 1,238,741 - -0.1 1,238,747 - +0.7 1,238,806 + -1.8 1,238,807 - -1.3 1,238,928 + -1.8 1,238,928 + -1.0 1,238,928 + +1.5 1,238,928 + -1.4 1,238,928 + -1.0 1,238,929 - -0.7 1,238,929 - -1.3 1,238,967 - DDA3937_RS05545 0.10 -0.3 1,239,026 + DDA3937_RS05545 0.14 +0.0 1,239,026 + DDA3937_RS05545 0.14 -1.3 1,239,026 + DDA3937_RS05545 0.14 +0.2 1,239,044 + DDA3937_RS05545 0.15 +0.9 1,239,044 + DDA3937_RS05545 0.15 +1.5 1,239,045 - DDA3937_RS05545 0.16 -0.1 1,239,045 - DDA3937_RS05545 0.16 -0.1 1,239,069 + DDA3937_RS05545 0.17 -0.8 1,239,198 + DDA3937_RS05545 0.26 -0.8 1,239,224 + DDA3937_RS05545 0.28 -0.5 1,239,225 - +0.3 1,239,225 - DDA3937_RS05545 0.28 +1.3 1,239,315 + DDA3937_RS05545 0.34 -0.5 1,239,402 + DDA3937_RS05545 0.40 -2.5 1,239,402 + DDA3937_RS05545 0.40 +0.2 1,239,402 + DDA3937_RS05545 0.40 -0.5 1,239,402 + DDA3937_RS05545 0.40 -0.8 1,239,402 + DDA3937_RS05545 0.40 -1.1 1,239,402 + DDA3937_RS05545 0.40 +1.1 1,239,403 - DDA3937_RS05545 0.40 -0.4 1,239,403 - DDA3937_RS05545 0.40 +0.3 1,239,403 - DDA3937_RS05545 0.40 -2.0 1,239,403 - DDA3937_RS05545 0.40 -2.2 1,239,403 - DDA3937_RS05545 0.40 -0.5 1,239,403 - DDA3937_RS05545 0.40 -2.9 1,239,435 + DDA3937_RS05545 0.42 +3.4 1,239,436 - -1.5 1,239,495 + DDA3937_RS05545 0.47 -2.8 1,239,496 - DDA3937_RS05545 0.47 -1.3 1,239,496 - DDA3937_RS05545 0.47 -0.1 1,239,654 + DDA3937_RS05545 0.58 -0.0 1,239,735 - DDA3937_RS05545 0.63 -1.8 1,239,779 + DDA3937_RS05545 0.66 -0.8 1,239,788 + DDA3937_RS05545 0.67 -0.3 1,239,788 + DDA3937_RS05545 0.67 -1.7 1,239,789 - DDA3937_RS05545 0.67 -1.0 1,239,789 - DDA3937_RS05545 0.67 -2.2 1,239,789 - DDA3937_RS05545 0.67 +0.5 1,240,129 - +2.1 1,240,173 + -0.5 1,240,173 + -0.8 1,240,173 + -1.6 1,240,221 + +1.7 1,240,258 - +0.7 1,240,350 + -1.8 1,240,350 + -2.8 1,240,351 - -0.8 1,240,356 + -1.4 1,240,356 + -1.7 1,240,357 - -1.1 1,240,357 - +3.2 1,240,402 + DDA3937_RS05550 0.11 +0.0 1,240,402 + DDA3937_RS05550 0.11 +1.2 1,240,402 + DDA3937_RS05550 0.11 +1.8 1,240,403 - DDA3937_RS05550 0.12 +0.5 1,240,561 + DDA3937_RS05550 0.38 +0.7 1,240,561 + DDA3937_RS05550 0.38 -2.8 1,240,561 + DDA3937_RS05550 0.38 -0.7 1,240,562 - DDA3937_RS05550 0.38 +0.7 1,240,562 - DDA3937_RS05550 0.38 -1.0 1,240,562 - DDA3937_RS05550 0.38 -0.4 1,240,562 - DDA3937_RS05550 0.38 -0.3 1,240,562 - DDA3937_RS05550 0.38 -2.5 1,240,721 + DDA3937_RS05550 0.65 -0.3 1,240,895 - -1.1 1,240,931 - -1.0 1,240,931 - -2.2 1,240,931 - -0.5 1,240,931 - +0.8 1,240,931 - -1.7 1,240,952 + -2.6 1,240,953 - -0.9 1,240,954 + -1.6 1,240,954 + -0.7 1,240,954 + +0.3 1,240,954 + -0.3 1,240,955 - -1.7 1,240,955 - -2.1 1,240,987 - DDA3937_RS05555 0.10 -2.8 1,241,018 - DDA3937_RS05555 0.14 -0.1 1,241,029 + DDA3937_RS05555 0.15 -1.4 1,241,030 - DDA3937_RS05555 0.15 -3.0 1,241,030 - DDA3937_RS05555 0.15 -1.5 1,241,117 - DDA3937_RS05555 0.24 -1.1 1,241,117 - DDA3937_RS05555 0.24 +0.3 1,241,117 - DDA3937_RS05555 0.24 +0.3 1,241,141 + DDA3937_RS05555 0.27 +0.6 1,241,142 - DDA3937_RS05555 0.27 -0.1 1,241,142 - DDA3937_RS05555 0.27 -1.1 1,241,142 - DDA3937_RS05555 0.27 -0.1
Or see this region's nucleotide sequence