Experiment: Stem-Norland
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DDA3937_RS14455 and DDA3937_RS14460 are separated by 230 nucleotides DDA3937_RS14460 and DDA3937_RS14465 are separated by 66 nucleotides DDA3937_RS14465 and DDA3937_RS14470 overlap by 1 nucleotides
DDA3937_RS14455: DDA3937_RS14455 - acetyl-CoA carboxylase, carboxyltransferase subunit beta, at 3,311,867 to 3,312,778
_RS14455
DDA3937_RS14460: DDA3937_RS14460 - DedA family protein, at 3,313,009 to 3,313,671
_RS14460
DDA3937_RS14465: DDA3937_RS14465 - tRNA pseudouridine(38-40) synthase TruA, at 3,313,738 to 3,314,568
_RS14465
DDA3937_RS14470: DDA3937_RS14470 - aspartate-semialdehyde dehydrogenase, at 3,314,568 to 3,315,578
_RS14470
Position (kb)
3313
3314
3315 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3312.815 kb on - strand at 3312.815 kb on - strand at 3312.823 kb on - strand at 3312.929 kb on - strand at 3312.993 kb on + strand at 3312.993 kb on + strand at 3312.998 kb on + strand at 3313.011 kb on - strand at 3313.011 kb on - strand at 3313.017 kb on + strand at 3313.018 kb on - strand at 3313.155 kb on - strand, within DDA3937_RS14460 at 3313.155 kb on - strand, within DDA3937_RS14460 at 3313.194 kb on + strand, within DDA3937_RS14460 at 3313.195 kb on - strand, within DDA3937_RS14460 at 3313.196 kb on + strand, within DDA3937_RS14460 at 3313.196 kb on + strand, within DDA3937_RS14460 at 3313.196 kb on + strand, within DDA3937_RS14460 at 3313.197 kb on - strand, within DDA3937_RS14460 at 3313.197 kb on - strand, within DDA3937_RS14460 at 3313.197 kb on - strand, within DDA3937_RS14460 at 3313.285 kb on + strand, within DDA3937_RS14460 at 3313.286 kb on - strand, within DDA3937_RS14460 at 3313.334 kb on + strand, within DDA3937_RS14460 at 3313.334 kb on + strand, within DDA3937_RS14460 at 3313.338 kb on + strand, within DDA3937_RS14460 at 3313.338 kb on + strand, within DDA3937_RS14460 at 3313.338 kb on + strand, within DDA3937_RS14460 at 3313.338 kb on + strand, within DDA3937_RS14460 at 3313.339 kb on - strand, within DDA3937_RS14460 at 3313.339 kb on - strand, within DDA3937_RS14460 at 3313.339 kb on - strand, within DDA3937_RS14460 at 3313.339 kb on - strand, within DDA3937_RS14460 at 3313.403 kb on + strand, within DDA3937_RS14460 at 3313.403 kb on + strand, within DDA3937_RS14460 at 3313.403 kb on + strand, within DDA3937_RS14460 at 3313.403 kb on + strand, within DDA3937_RS14460 at 3313.404 kb on - strand, within DDA3937_RS14460 at 3313.404 kb on - strand, within DDA3937_RS14460 at 3313.599 kb on - strand, within DDA3937_RS14460 at 3313.599 kb on - strand, within DDA3937_RS14460 at 3313.654 kb on - strand at 3313.675 kb on - strand at 3313.686 kb on + strand at 3313.687 kb on - strand at 3313.734 kb on - strand at 3313.739 kb on + strand at 3313.953 kb on + strand at 3313.953 kb on + strand, within DDA3937_RS14465 at 3313.994 kb on + strand, within DDA3937_RS14465 at 3314.141 kb on + strand, within DDA3937_RS14465 at 3314.227 kb on - strand, within DDA3937_RS14465 at 3314.489 kb on + strand at 3314.507 kb on + strand at 3314.579 kb on - strand at 3314.579 kb on - strand at 3314.746 kb on + strand, within DDA3937_RS14470 at 3314.824 kb on - strand, within DDA3937_RS14470 at 3314.913 kb on + strand, within DDA3937_RS14470 at 3315.115 kb on - strand, within DDA3937_RS14470 at 3315.203 kb on + strand, within DDA3937_RS14470 at 3315.378 kb on + strand, within DDA3937_RS14470 at 3315.378 kb on + strand, within DDA3937_RS14470 at 3315.378 kb on + strand, within DDA3937_RS14470 at 3315.476 kb on - strand, within DDA3937_RS14470
Per-strain Table
Position Strand Gene LocusTag Fraction Stem-Norland remove 3,312,815 - -1.9 3,312,815 - -2.1 3,312,823 - -0.1 3,312,929 - -0.7 3,312,993 + +0.4 3,312,993 + +0.3 3,312,998 + -0.3 3,313,011 - +0.2 3,313,011 - -2.7 3,313,017 + -2.9 3,313,018 - +0.4 3,313,155 - DDA3937_RS14460 0.22 -0.6 3,313,155 - DDA3937_RS14460 0.22 +1.9 3,313,194 + DDA3937_RS14460 0.28 +0.0 3,313,195 - DDA3937_RS14460 0.28 +0.3 3,313,196 + DDA3937_RS14460 0.28 -1.2 3,313,196 + DDA3937_RS14460 0.28 -0.2 3,313,196 + DDA3937_RS14460 0.28 +0.2 3,313,197 - DDA3937_RS14460 0.28 +1.3 3,313,197 - DDA3937_RS14460 0.28 +0.3 3,313,197 - DDA3937_RS14460 0.28 -1.2 3,313,285 + DDA3937_RS14460 0.42 -0.3 3,313,286 - DDA3937_RS14460 0.42 +1.4 3,313,334 + DDA3937_RS14460 0.49 +0.3 3,313,334 + DDA3937_RS14460 0.49 +0.4 3,313,338 + DDA3937_RS14460 0.50 -0.4 3,313,338 + DDA3937_RS14460 0.50 +0.5 3,313,338 + DDA3937_RS14460 0.50 -0.7 3,313,338 + DDA3937_RS14460 0.50 -0.7 3,313,339 - DDA3937_RS14460 0.50 -1.0 3,313,339 - DDA3937_RS14460 0.50 +0.2 3,313,339 - DDA3937_RS14460 0.50 +0.1 3,313,339 - DDA3937_RS14460 0.50 -0.1 3,313,403 + DDA3937_RS14460 0.59 +0.6 3,313,403 + DDA3937_RS14460 0.59 +0.3 3,313,403 + DDA3937_RS14460 0.59 +0.9 3,313,403 + DDA3937_RS14460 0.59 -0.6 3,313,404 - DDA3937_RS14460 0.60 +0.1 3,313,404 - DDA3937_RS14460 0.60 +0.0 3,313,599 - DDA3937_RS14460 0.89 -0.9 3,313,599 - DDA3937_RS14460 0.89 +1.4 3,313,654 - +0.6 3,313,675 - -0.1 3,313,686 + -1.5 3,313,687 - -0.7 3,313,734 - -0.7 3,313,739 + -1.5 3,313,953 + -0.3 3,313,953 + DDA3937_RS14465 0.26 -1.7 3,313,994 + DDA3937_RS14465 0.31 -1.1 3,314,141 + DDA3937_RS14465 0.48 -1.3 3,314,227 - DDA3937_RS14465 0.59 -1.7 3,314,489 + +0.3 3,314,507 + -2.4 3,314,579 - -0.4 3,314,579 - -0.5 3,314,746 + DDA3937_RS14470 0.18 -2.7 3,314,824 - DDA3937_RS14470 0.25 -1.6 3,314,913 + DDA3937_RS14470 0.34 -0.9 3,315,115 - DDA3937_RS14470 0.54 -1.7 3,315,203 + DDA3937_RS14470 0.63 -1.2 3,315,378 + DDA3937_RS14470 0.80 -0.2 3,315,378 + DDA3937_RS14470 0.80 -1.4 3,315,378 + DDA3937_RS14470 0.80 -0.7 3,315,476 - DDA3937_RS14470 0.90 +0.9
Or see this region's nucleotide sequence